New every week
If you know of other current amphibian-related news or papers that would be of interest here, please drop us a note.
For AmphibiaWeb's list of current papers related to amphibian declines and amphibian conservation, updated monthly by Tim Halliday, see our Recent Scientific Publications page.
- June 27, 2016:
To gain a better understanding of the African genus Amietia, which is currently composed of 16 cryptic species, Larson et al. (2016) recently analyzed the genus using newly collected samples from The Albertine Rift, which is home to five Amietia species, and published species sequences. They showed that Amietia is monophyletic, diverged in the late-Oligocene to early-Miocene (17.44–31.97 mya), and contains more species than previously thought. Amongst the 79 samples collected, a known vertebrate biodiversity hotspot, they found six undescribed species. All of the new species were found in unprotected land and all the Amietia species in the Rift are currently threatened by political instability, habitat loss, and chytridiomycosis. Although political instability makes it difficult for researchers to gain access to areas where these undescribed species exist, the researchers are optimistic that more undescribed species can be found there and urge future surveys and conservation efforts in the area. (AChang)
- June 20,2016:
A new study (Willaert et al. 2016) presents the first details of the reproductive behavior of frogs of the Indian endemic family Nyctibatrachidae. There are several surprises. The subject of the study, Nyctibatrachus humayuni, is a stream-breeder. Both males and females call, although the latter is rare and of unclear function. Males call from territories that may be partly submerged, but usually are on rocks, vegetation and fallen trees bordering or overhanging the stream; territories were observed up to 4 m above water. A female approaches a male, who mounts the female without using a grasping amplexus, instead using the hands to hold onto vegetation, thus supporting the pair. This is termed a "dorsal straddle". This loose embrace is short in duration. Immediately after the male dismounts, the female lays the full clutch in a few seconds, without any physical contact with the male. The eggs when collected immediately are already fertilized, leading to the hypothesis that the male spreads sperm over the surface of the female's back and legs, enabling fertilization as the eggs are laid. (DW)
- June 13, 2016:
The hylid frog fauna in parts of South America is sufficiently rich to permit recognition of different locomotor modes and habitats, suggesting ecomorphs. In an impressive new paper, Soliz and Ponssa (2016) studied 14 hylids that differed in locomotion and habitat. Biomechanical analysis focused on relationships between ligaments and sesamoid skeletal elements. A special role for heterochrony is suggested. They tested hypotheses of adaptation versus common ancestry using a phylogenetic analysis, and present evidence for both effects. This detailed, well-illustrated study will likely prove to have heuristic value for other taxa and regions. (DW)
- June 6, 2016:
Eastern North American woodlands have been invaded by Asian earthworms, potent ecosystem engineers. They accelerate leaf litter decomposition and nutrient release, consume detritus, and alter edaphic properties, all potentially significant to co-occurring salamanders. An experimental lab and field study (Ziemba et al. 2016) examined the impact of invading earthworms on Eastern Red-backed Salamander, Plethodon cinereus, in Ohio. Salamanders use lower quality microhabitat and consume fewer prey in the presence of earthworms, and behave aggressively toward earthworms. Earthworms and salamanders share cover objects less often than random expectations. Earthworm abundance was negatively associated with abundance of some salamander classes. Loss of cover and physical exclusion of salamanders likely hinders salamander performance, Thus, a scenario following earthworm invasion is reduced recruitment and abundance. While P. cinereus is widespread and abundant and is not under immediate threat, related species with restricted geographic ranges might well face great threat from future earthworm invasion of their isolated ecosystems. (DW)
- May 30, 2016:
Over 25 years ago when researchers first began tackling the problem of global amphibian declines, studies began by focusing on single factor hypotheses that might explain global patterns. Since then, many studies have proposed that multiple factors may be responsible. The USGS Amphibian Decline Working Group (Grant et al 2016) studied 61 documented areas in North America covering 83 species to test the impact of four hypothesized large-scale factors: human disturbance, chytridiomycosis, pesticide effects, and climate change, especially in rainfall. They found that each stressor was regionally variable but did not explain declines at a continental scale. They conclude that the best conservation strategy must emphasize local solutions rather than a large-scale comprehensive approach. (VV, MK)
- May 23, 2016:
How long do salamanders live? Staub (2016) reviews evidence for salamanders of the family Plethodontidae, and concludes that these generally small animals can attain relatively great age. The most direct evidence is from captive animals, and members of Plethodon, Aneides and Phaeognathus, among others, have attained ages in excess of 30 years (the individual in the photograph was collected as an adult in April 1985, and is still apparently in robust health). In a separate field study (Staub 2016a), Aneides flavipunctatus longevity was estimated to be on the order of 18 or 25 years, using different methods. Other field estimates also show long lives, as great as 32 years. Methods tend to be conservative, and some individuals might be substantially older. (DW)
- May 16, 2016:
The Paradoxical Frogs, genus Pseudis, are so-named because the tadpoles of the first-named species, P. paradoxa, are enormous, larger than the adults. The tadpoles have bodies that are ovoid in general shape and very large; in lateral view, tadpoles are triangular in outline and higher than wide, with well-developed tail musculature. A new study focused on P. fusca and P. tocantins (Santana et al. 2016) demonstrates that, like other species of Pseudis studied so far, they have typical tadpoles, although each of the six species studied has distinctive features associated with the oral disc. Despite their extraordinary tadpoles, Pseudis is in the family Hylidae. (DW)
- May 7, 2016:
Saturday, May 7, has been declared Salamander Saturday, a day to promote international awareness of salamanders and to promote their conservation. The Foundation for the Conservation of Salamanders has developed activities for this day throughout the US and in Canada. We celebrate salamanders everyday. Learn more about salamanders on AmphibiaWeb. See some Amazing Salamander profiles from our Amazing Amphibian archives.
- May 2, 2016:
Duellman, Marion and Hedges (2016) offer an alternative taxonomy for hylid frogs. No new data are presented and the authors’ goal, to use taxonomy to reflect biogeographic divergence, can often be accomplished by use of subgenera. AmphibiaWeb will move methodically to accept or reject specific features of this taxonomy, which is purely subjective and not required by any cladistic or nomenclatural considerations. We invite your views via Twitter or Facebook in the coming weeks. Examples of the proposed taxonomy: Hylidae is split into three families; Hyla is subdivided, with the effect that it no longer occurs in the New World; western tree frogs are placed in a newly genus; new genera are established for some tropical clades.
- April 25, 2016:
Besides making its hosts sick, a pathogen may also alter the host's behavior. An and Waldman (2016) found that Japanese Tree Frogs (Hyla japonica) with natural infections of Batrachochytrium dendrobatidis called longer and made more rapid calls than did uninfected males. This may have two effects, possibly interrelated. Because female frogs are known to be generally attracted to males that call longer and faster, pathogen transmission might be enhanced. Alternatively, the altered calls might result from selection on males for early reproductive success in anticipation of a premature death. However, it is unknown whether Japanese tree frog females prefer infected males. (DC)
- April 18, 2016:
Signaling between parents and offspring is a topic of interest in animal behavior and evolution, mostly in birds and mammals. A common question is whether offspring begging directed at parents represents an “honest signal” of need (hunger), or a signal of quality (with larger, higher quality offspring able to signal more strongly). Yoshioka, Meeks and Summers (2016) tested this question in Ranitomeya imitator, the Mimic Poison Frog, which shows pair-bonding and biparental care. The male and female cooperate to place tadpoles in tiny pools of water (phytotelmata), and then return to feed the tadpoles infertile eggs as it develops. They showed regulating food levels across development, tadpoles receiving less food increased begging levels significantly over the course of development, relative to tadpoles given more food. An experiment manipulating the amount of begging that tadpoles perform (under identical feeding regimes) revealed costs of begging in terms of developmental rate and growth. An experiment on parental feeding revealed that parents preferentially fed tadpoles not receiving supplemental food, relative to siblings whose diet was supplemented with extra food. They conclude that tadpole begging in this species serves as an honest signal of need. (Kyle Summer)
- April 11, 2016:
Although general convention says that stress reduces reproduction, a species’ length of breeding season and its lifespan also play a role. Woodley and Porter (2015) recently tested the interaction of stress, length of breeding season, and lifespan in Spotted Salamanders, Ambystoma maculatum, which are long-lived, explosive breeders, by comparing the time it took males to deposit spermatophores (sperm packages that females use to fertilize eggs) and how many spermatophores were dropped in males deliberately stressed by handling and control males. They found that, while there was not a significant difference in how long it took males to drop spermatophores, stressed males deposited significantly more. The authors suspect that this may be a strategy to increase reproductive potential when there is a greater risk to survival. (AChang)
- April 4, 2016:
The Rough-skinned Newt,Taricha granulosa, is engaged in an evolutionary arms race with its only known significant predator, the Common Garter Snake, Thamnophis sirtalis. In regions where snakes are absent (such as some islands near Vancouver Island, Canada), newt toxicity is low to absent, whereas in sites where toxicity-resistant snakes are common (various sites in California and Oregon), newt toxicity is high to very high. The authors of a new paper (Hague et al. 2016) studied newts in southeast Alaska, where snakes are absent, and as expected, toxicity levels were low at most sites examined. However, puzzling variation was found. In one lake on Wrangell Island, no toxicity was found, but newts from another lake on the same island displayed surprisingly high levels, rivaling those in some areas where snake predators have high toxin resistance. Various explanations are offered, but reciprocal selection does not fully explain the toxicity variation in newts. (DW)
- March 28, 2016:
A new report from Europe (Spitzen-van der Sluijs et al 2016) shows that the introduced chytrid pathogen, Batrachochytrium salamandrivorans (Bsal), spread from the Netherlands and Belgium into Germany, as previously predicted. This pathogen was only recently discovered and is related to the the fungal pathogen Batrachochytrium dendrobatidis (Bd) that has decimated amphibians globally as it has spread into naive populations. They analyzed 1019 skin swab samples collected from 55 sites. The infected animals were found at 7 sites in the Netherlands, 4 sites in Belgium, and 3 sites in Germany (total infected sites 14 of 55 sites). The study is important because it provides important data from the field on the current distribution of Bsal, and provides benchmark data extremely useful for the basic understanding of the dynamics of the host pathogen interaction for this newly emerging pathogen. (VV)
- March 21, 2016:
Most family-level radiations of frogs are continental. One of the few island archipelago radiations is the Ceratobatrachidae, a clade of 96 species on the islands of Southeast Asia and the Southwest Pacific. However, the closest relative of ceratobatrachids is unknown, and so the biogeographic origin of the clade is unclear. Yan et al. (2016) discovered that three species of Limnonectes (family Dicroglossidae) are more closely related to ceratobatrachids than to other taxa. Surprisingly, these species are from mainland China, where no ceratobatrachids were previously known. This discovery provides a biogeographic link for an enigmatic radiation of island frogs. (DC)
- March 14, 2016:
Plethodontid salamanders are more agile than other salamanders and they are known to jump, especially to escape predation. Ryerson et al. (2016) studied jumping in six species (three genera). All were capable of jumping by lateral body bending followed by rapid straightening, which propels the salamander into the air. Most likely force to propel the animal is applied through one or both hind limbs. Salamanders jumped a maximum of 1.8 times head+body length and jump height is scaled with body size and forelimb length. Species did not differ in any other aspects of performance, except takeoff velocity where longer salamanders are slower. No correlation was found between tail length, or even presence of a tail, and any variable. Further kinematic analysis is needed to understand how salamanders maintain jumping performance no matter the size. (DW)
- March 7, 2016:
How will amphibians respond to global warming? A new study by Orizaola and Laurila (2016) finds that three populations of the Pool Frog (Pelophylax lessonae) from the northernmost populations, isolated in the Uppland district north of Stockholm, Sweden, display greater thermal phenotypic plasticity than three populations from Poland (about the center of the species distribution) and Latvia (2 from northern-edge populations). The Uppland populations show greater temperature-induced plasticity affecting duration of the larval period, mass at metamorphosis, and growth rate, than other populations. All these variables are important in facilitating positive responses to periodic warm spells in colder environments with shorter growing seasons. Geographic variation in plasticity of life history components should be considered in predicting responses of particular species to climate change. (DW)
- February 29, 2016:
One of the most widespread amphibians has thought to be the Southeast Asian common toad, Duttaphrynus melanostictus, considered a human commensal that has widely spread by human actions. It was thus a surprise when Wogan et al. (2016) reported a high level of geographic and genetic structure in the species and the discovery of three distinct lineages: Asian mainland, coastal Myanmar, and Sundaic islands. These represent at least three and possibly multiple species, which have higher conservation value than currently recognized. The species complex has been introduced to such distant places as Borneo, Sulawesi and Seram, and even Madagascar. Which species have been introduced and what their ecological niches, geographic ranges, and dispersal potentials remain to be determined. (DW)
- February 22, 2016:
Tim Halliday has produced a beautiful book on frogs (The Book of Frogs: a life-size guide to six hundred species from around the world, Ivy Press). Following 37 pages of short but informative sections on aspects of frog biology, species accounts begin. The presentation is comprehensive and cosmopolitan, following a skeletal phylogeny from AmphibiaWeb. Each is headed by a short but informative data table and a map of the world showing distribution. Each account shows a full-size photo, and for all small species a large detailed photo, with carefully but interestingly written introductions to the species and information about similar species. Each account has a small drawing that enhances the presentation. The photos, mainly from AmphibiaWeb photographers, are well-chosen. All families and a surprisingly large number of genera are represented. This is a very entertaining and informative book –highly recommended!
- February 15, 2016:
Birds and mammals are well-known for their behavioral flexibility and learning aptitudes, but these traits are not as well-characterized in amphibians. Liu et al. (2016) investigated these traits in the poison frog Dendrobates auratus, a species with complex reproductive strategies that may favor highly flexible forms of learning. Experiments on serial reversal learning using a two-sided maze with distal visual cues revealed that the frogs could learn to identify the correct exit. Probe trials in which visual cues were switched demonstrated that the frogs relied on these cues. Serial reversals demonstrated that the frogs learned to learn more rapidly across trials, employing rule-based strategies to solve the maze with increasing rapidity, thus demonstrating high levels of behavioral flexibility and learning ability in an amphibian. (Kyle Summers)
- February 8, 2016:
Spadefoot toads (family Scaphiopodidae) are a familiar part of the North American frog fauna and relatively common in the North American fossil record. Chen et al. (2016) fill in an important gap in our understanding of this group with a fossil from the late Paleocene of Mongolia. Their work suggests that the two genera of living spadefoots (Scaphiopus and Spea) last shared an ancestor near the end of the Cretaceous. They also suggest that the presence of this early spadefoot frog in Asia supports dispersal into North America via Beringia in the Early Cenozoic. (DB)
- February 1, 2016:
In a new review, Alessandro Catenazzi (a member of the AmphibiaWeb Team) assesses the state of the World’s amphibians. The paper, filled with new information and ideas, is a thorough introduction to the issues involved in amphibian declines and attempts to combat them. The main focus is on immediate and new threats, ranging from infectious diseases to climate change. The status of amphibian conservation activity is surveyed and continuing threats (as from the recently discovered and apparently salamander-specific chytrid fungus) are evaluated. Synergistic effects are highlighted, as between disease and climate change. Catenazzi argues for more field studies of amphibian physiological ecology (for example, critical thermal limits). He discusses paradoxes, such as the discovery rate of new species while the taxon as a whole declines. His conclusion is that humans will need all their ingenuity, motivation and moral impetus to save amphibians. (DW)
- January 25, 2016:
Parental care in anurans usually takes the form of attendance of eggs. Male attendance of eggs laid on vegetation has evolved independently in two genera of the glass frogs, family Centrolenidae: within Centrolene and within Hyalinobatrachium. Now Valencia and Delia (2016) have discovered parental care in a third clade of centrolenids, Ikakogi tayrona, the geographically (Sierra Nevada de Santa Marta, Colombia) and phylogenetically isolated sister-taxon of all other centrolenids. Unexpectedly, the attendance in Ikakogi tayrona is by females. Care functions are similar in both males and females, but females are somewhat more risk-tolerant, probably reflecting the more direct link to fitness in females. (DW)
- January 18, 2016:
A reflection of the gravity with which the scientific community regards the Bsal “salamander-devouring” pathogen, the US Fish and Wildlife Service has issued an unprecedented ruling to address this biodiversity crisis. The ruling implements the Lacey Act by listing as injurious to wildlife, and thereby banning, 201 salamanders, mainly Asian species believed to be Bsal carriers and popular in the pet trade, from importation and interstate transportation. By specifically targeting the salamander species which are suspected to be susceptible to and carriers of the new chytrid fungus, the US FWS aims to protect the global hotspot for salamander diversity in North America, centered mainly in the US. The ban, broad in its scope yet specific in its named species, has yet to be tested as written to strike the balance between protection from an emerging infectious disease and ongoing research, which frequently requires transportation of samples and specimens. The interim ban is effective on January 28th and an open commentary period is available until March 14th. (MK)
- January 12, 2016:
A massive experiment in extinction prevention is underway in Panama, where many organizations and individuals are involved in a captive breeding program. An evaluation of the program (Gratwicke et al 2015) reports that 18 species are being held for conservation purposes, with numbers in captivity ranging from over 1,000 (Atelopus zeteki) to 1 (a single male of Ecnomiohyla rabborum). Of these, 5 are Atelopus species and the 6th is Gastrotheca cornuta and each are represented by at least 10 males and 10 females. An expert survey analysis (based on 1387 responses from experts for 215 Panamanian amphibians) ranks all species according to their perceived susceptibility to chytrid infection, probability of finding founders, probability of going extinct in the wild, and probability of avoiding extinction through captive breeding programs (using lower and upper bound for the three probabilities). The probability of successful captive breeding is medium to high for about 2/3rds of the Panamanian species but the challenge is daunting. Already about 4% of the species are missing. They conclude captive breeding could improve the odds of avoiding extinction for some species that have declined. Many of the species (notably salamanders, caecilians) are too rarely encountered to be part of the program. (DW)
- January 4, 2016:
Starting the new year on a positive note: Woodhams and colleagues (2015) recently created the Antifungal Isolate Database, nearly 2,000 strains of naturally occurring cutaneous bacteria with anti-fungal properties that are found on amphibians. This database will help researchers and managers select the appropriate strain for bioaugmentation to combat chytridiomycosis infections, help researchers find new strains with anti-fungal capabilities during whole genome sequencing, and predict how new strains will function. Two files are available are available now: (1) a FASTA file of the bacterial isolates’ 16S rRNA gene sequences and (2) a metadata of the 37 host species, host life stage, geographic region (12 countries from 5 continents), and the taxonomic identity and anti-fungal capacity of each strain. Once launched, they hope to continue to add new projects to the database, which could prove to be a powerful new tool in amphibian conservation. (AChang)
- December 28, 2015:
2015 has been a good year for AmphibiaWeb-- we’ve seen our species accounts grow to 33% of all known amphibians (over 3,100 species accounts in total), which has also increased by 115 new species described in 2015. Our new accounts include French excerpts from Jean Raffaëlli’s 2nd edition of Les Urodèles du Monde for all salamanders, and contributions from students in seven herpetology classes around the US. Most importantly, we saw heightened urgency for a call to action against the latest threat to salamanders worldwide from a recently described chytrid fungus, Bsal. AmphibiaWeb and the US Forest Service joined a national task team to develop an Amphibian Disease portal to aggregate data and coordinate efforts in the field and lab to understand how to combat this emerging infectious disease. In 2016, we will launch this effort and will need your support to make a difference in amphibian conservation. Happy New Year's!
- December 21, 2015:
What were the musculoskeletal changes needed to transverse from water-dwelling locomotion to the much more demanding terrestrial life about 390 million years ago? Kawano et al (2015) asked this of the earliest tetrapod and examined Tiger Salamanders (Ambystoma
tigrinum) as proxy. Specifically they measured bone loadings on the fore and hind limbs in a variety of ways assessing the 'safety factor' or margin of extra protection that a limb will take in excessive loads, and found differences when comparing the humerus and femur bones in the stresses they can withstand and experience while walking. Learning how these salamanders, which have similar morphology to the early tetrapods, locomote will allow better modeling and understanding of the biomechanics of our ancestors when they invaded land. (MK)
- December 14, 2015:
A new paper (Bosch et al. 2015) has garnered much attention because of its strong claim: the research team eradicated Bd, the pathogenic fungus of amphibians, from the island of Mallorca in the western Mediterranean. The small island harbors a single species, Alytes muletensis, discovered only about 35 years ago, and there are few breeding sites, mostly in nearly inaccessible deep canyons. Bd appeared a few years ago, probably arriving through human agency, and threatened the species. The team used a common fungicide (itraconazole) and with considerable effort eliminated infection from organisms and the environment; it took five years but it worked – so far. While this is an important proof of concept, application to other parts of the world is problematic. Environmental impacts of the treatment are left unexplored, and the problems of multispecies communities, direct developing species, species with multi-year larvae, among other issues, may render this approach of limited value. (DW)
- December 7, 2015:
A new approach to Bd as a global pathogen of amphibians is proposed by a team of active researchers. As an alternative to focusing on “hot spots” of chytrid infection, James et al. (2015) recognize that it is a global problem and argue for a reframing of questions related to distribution, so instead ask: “where are the cold spots of Bd distribution?” “why are they cold?” These could be artifacts of ignorance, or Bd might never have been present in these areas. Environmental conditions may be outside the tolerance window for Bd. They also suggest that Bd might have been present historically, but frogs evolved defenses (or only resistant species survived pathogen-driven selection); Bd later disappeared or persists at low prevalence. They refer to this as the “Ghosts of Epizootics Past” hypothesis and suggest that an important lesson learned so far is never stop sampling. They suggest that Korea might serve as a compelling control population because Bd is now enzootic in its amphibian fauna. (DW)
- November 30, 2015:
Disease dynamics are rarely simplistic and often difficult to reconstruct historically; chytridiomycosis is no exception. Sette et al 2015 examined chytrid prevalence using both historic museum and current field samples of the California Slender salamander, Batrachoseps attenuatus. From random samplings of about 30k museum specimens, they found that Bd emerged rapidly in Northern California and exhibited a non-linear pattern of spread. By resampling the historic positives sites, they were able to determine that modern-day persistence of Bd was negatively correlated with time since first detection of infection, suggesting that recent invasions were more likely to persist, and positively correlated with proximity to lentic aquatic habitat, suggesting that aquatic carriers of Bd may transfer it to terrestrial B. attenuatus populations. Interestingly, sociality was also suggested as an important factor in disease spread and populations exposed earliest to Bd showed lower probability of aggregation behavior in the field. (MK)
- November 23, 2015:
The expansion of Cane Toads (Rhinella marina) in Australia is well known, but the species has been widely introduced elsewhere as well. A new study (Narayan et al. 2015) focuses on the impact of its introduction into Fiji on the native frogs. This experimental study of frogs in enclosures and in nature showed that Fiji Ground Toads (Cornufer vitianus, formerly Platymantis vitiana) were adversely affected by the invasive species, which exploits broader ecological roles and has strongly negative indirect effects. Most importantly, there is a significant reduction in reproductive success, the probable result of high stress levels as reflected in hormone measures. (DW)
- November 16, 2015:
Defenses against predators are assumed to promote speciation because the defense provides a release from predation. Arbuckle and Speed (2015) examined the macroevolutionary effects of chemical defense and conspicuousness in amphibians and found that for conspicuous species the speciation rate was 2-3 times higher than for cryptic species, but the extinction rate was the same. In chemically defended amphibians, they show speciation rates were about 2 times that for undefended species. Surprisingly, however, the extinction rate was three times higher in lineages with chemical defense than without it. The authors suggest several explanations for this as well as suggest that understanding defensive traits may be instructive to predicting extinction. As important may be opening the discussions about conservation policies applied to higher at risk, chemically defended species and how we categorize them. (DC)
- November 9, 2015:
On November 8, 2015, Peru established an enormous new national park in the heart of the Amazon Basin. Parque Nacional Sierra del Divisor encompasses 1,335,000 hectares and abuts the Brazilian national park Serra do Divisor (about 843,000 hectares). Together both parks protect more than 2.14 million hectares (an area larger than the states of Connecticut and Rhode Island combined). Herpetologists M. Barboza de Souza and C. Rivera found 67 species of frogs and one salamander in 16 days of work during a Rapid Biological Inventory conducted in 2005 (Field Museum, RBI 17); doubtless many more amphibians occur in the area. The new park protects an area of diverse topography, highlighted by a scattering of impressive old volcanos. The actions of the governments of Peru and Brazil to preserve a vast area of extraordinary biodiversity value also protect the ancestral territories of several Amazonian indigenous groups. (DW)
- November 2, 2015:
An international team, composed of field biologists from the Palawan Centre of Sustainability, the National Museum of the Philippines, the Palawan Council for Sustainable Development, and the University of Kansas has recently rediscovered the long-lost Malatgan Caecilian, Ichthyophis weberi, from north-central Palawan Island, western Philippines. Discovered in the early 1900s, this species has not been observed in the field since the 1970s. Thought to have been possibly extinct, I. weberi may be a dry microhabitat specialist (found away from water in dry forest floor detritus), which has escaped detection by biologists who have searched in vain for the species in moist, riparian, or semi-aquatic microhabitats. The expedition also rediscovered the Palawan toadlet (Pelophryne albotaeniata), not seen in the last 40 years. (Rafe Brown)
- October 26, 2015:
The Amphibian Survival Alliance (ASA) and their partners have been hard at work creating protected areas. In August, they announced the creation of Ankaratra Massif Reserve in Madagascar, which was the result of a five-year effort to protect rare Madagascan endemics, such as William's Bright-eyed Frog (Boophis williamsi) among other amphibians and reptiles. In September, they announced the creation of the San Isidro Amphibian Reserve in Guatemala, where, in 2009, an international team of scientists, including UC Berkeley's Ted Papenfuss and Sean Rovito, rediscovered the Finca Chiblac Salamander, Bradytriton silus, and the Long-limbed Salamander, Nyctanolis pernix, which had not been seen in over 30 years. Still missing since its discovery in 1975 is the Jackson's Climbing salamander, Bolitoglossa jacksoni, also presumed endemic to the Sierra de los Cuchumatanes. With protection, we have hope to rediscover that species, too. (AChang)
- October 19, 2015:
Although the chytrid fungus, Batrachochytrium dendrobatidis (Bd), has been known to cause amphibian die-offs since the 1990’s, little work has been done to assess the long-term ecological consequences of disease-driven amphibian extirpation. Rantala et al. (2015) surveyed a Panamanian stream to determine if and how the structure and function of the aquatic ecosystem had changed over the 8 years since an amphibian die-off. They found a decrease in coarse organic matter and species richness of both macroinvertebrate and tadpoles. Furthermore, changes were seen in the nitrogen cycle that was not immediately apparent after the die-off. Interestingly, there was no evidence of functional redundancy or compensation by other species after the loss of amphibians, even after 8 years. The authors urge more studies of disturbed ecosystems over longer periods of time that measure multiple ecosystem functions to understand ecosystem processes. (AChang)
- October 12, 2015:
The recently described second species of pathogenic fungus, Batrachochytrium salamandrivorans (Bsal), was originally found in the southeast of The Netherlands and subsequently found in Belgium causing mass die-offs of fire salamanders. However, it has not yet been reported in other countries in natural settings. Sabino-Pinto et al 2015 reports a widespread infection of Salamandra (in four different species and 12 subspecies) in a captive-breeding facility in Germany. The source of the infection is unclear, but the very fact that Bsal is present in captive animals and that these animals may have been distributed elsewhere is deeply disturbing, and bears out the hypothesis of its possible spread from Asia to Europe. The paper also highlights the pivotal role of the pet trade industry in combatting this threat. (DW)
- October 5, 2015:
Many cases of overexploitation of amphibian species remain poorly documented because exploitation and consumption happen at very local scales. Recent work by Thomas & Biju (Salamandra 2015) extensively documents tadpole consumption in the endangered purple frog, Nasikabatrachus sahyadrensis – the sole species of the Nasikabatrachidae described in 2003. At a single study site in the Western Ghats of India, thousands of tadpoles were harvested per year, as well as adults. While potentially sustainable, the combination of heavy exploitation with annual variation in monsoon rains likely have a long-term negative impact on this threatened and biologically unique species. (DB)
- September 28, 2015:
Endocrine-disrupting chemicals in the environment are known to affect sex differentiation of amphibians during development. Agricultural pesticides are the most familiar environmental endocrine disruptors, but others include synthesized and natural estrogens (animal and plant). Lambert et al. (2015) found that in metamorphic Rana clamitans the bias toward females was higher in suburban ponds than in forested ponds. As well, the amount of suburban land use (residential landscaping) was related to higher levels of phytoestrogens (plant estrogens) in suburban ponds compared to forest ponds. This suggests that changes in land use are unappreciated sources of estrogen exposure to wildlife. (DC)
- September 21, 2015:
Conservation policy makers rely on the IUCN status of amphibians, of which the designation Data Deficient is the default category for species lacking information for placement. However, DD may be too simplistic, obscuring species that are safe with the threatened. Nori and Loyola (2015) quantify these DD species’ spatial relations to protected areas and heavily human-modified landscapes, and found that on average 81% of DD species’ ranges fall outside protected areas with much of it in human-modified landscapes, up to 58%. Regional variation among Latin America, SE Asia, Africa and Oceania is high and often alarming given the socio-political trends in increases of human-modified landscapes and decreases in protected areas. Their analysis of DD species gives a starting point to address otherwise ignored species. (MK)
- September 14, 2015:
The Central American amphibian fauna may be the richest in the world per unit area and it is one of the most endangered. Wilson et al. (2015; direct link to pdf) evaluate the status of the entire herpetofauna of the region, using their revised Environmental Vulnerability measure, which assigns scores (EVS) of 3 – 20 for all species. They assert: “We regard the IUCN system as expensive, time-consuming, tending to fall behind systematic advances, and over-dependent on the Data Deficient and Least Concern categories. Conversely, the EVS measure is economical, can be applied when species are described, is predictive, simple to calculate, and does not “penalize” poorly known species”. Of the 493 species of amphibians in the region, 70.8% have scores in the high vulnerability category (EVS of 14-20) and 92.4% of 159 salamanders evaluated are in that category. (DW)
- September 6, 2015:
Of the three orders of amphibians, Australia only has one native order, frogs (Anura). However, that changed when Tingley et al. (2015) recently reported on the successful establishment of the European newt, Lissotriton vulgaris in southeastern Australia. The introduction of L. vulgaris is believed to have occurred from escaped or illegally released pets before the species was listed as a "controlled pest animal" and its import subsequently prohibited. The potential ecological implications of this invasion are great as the European newt competes with native frogs and has the potential of producing a neurotoxin that to native predators have no evolutionary history with. Lastly, because the climate in southeastern Australia is similar to the native range of the species, L. vulgaris has the potential to spread across much of southern Australia and Tasmania. (AChang)
- August 31, 2015:
The large (nearly 400 species) family Rhacophoridae, widespread in the Asian subtropics and tropics with a few species in Africa, displays a great array of life history modes. A multigene, phylogenetic analysis of all major clades and nearly a third of the species in the family has enabled Meergaskumbura et al. 2015 to conclude that the ancestral mode was fully aquatic eggs and larvae. The ancestral forms of all but the most basal branches in the tree have a high likelihood of having been terrestrial gel nesters. Direct development and foam nesting characterize the vast majority of rhacophorids and have evolved independently at least twice within the family. As a probable consequence of these evolutionary events, rhacophorids thrive in diverse habitats across a vast geographic range. (DW)
- August 24, 2015:
To better understand why the fungal pathogen, Batrachochytrium dendrobatidis (Bd), varies in mortality outcome with different species, Stevenson et al. (2014) explored how host-specific body temperature at low and high elevations contribute to host Bd response. The authors incubated Bd cultures that mimicked temperatures experienced by three sympatric Litoria species, L. nannotis, L. rheocola, and L. serrata. The culture growth patterns were consistent with recovery rates in nature where the most varied thermal regime, associated with Litoria serrata at low elevation, had slower culture growth rates and a faster species recovery rate in the wild than L. nannotis and L. rheocola, which have a stable and less varied thermal regime, respectively. Their findings support the idea that natural temperature regimes affect the patterns of Bd-driven decline. (AChang)
- August 17, 2015:
No salamanders are known to occur on any of the Caribbean islands. Now a salamander has been found in a piece of amber from the Dominican Republic on the island of Hispaniola. The salamander, a tiny hatchling, has been named Palaeoplethodon hispaniolae, and it is thought to be a member of the family Plethodontidae, and in particular its clade Bolitoglossini, which includes more than 40% of all salamanders (Poinar and Wake, 2015). Bolitoglossines live exclusively in the New World tropics. The amber is dated between 15 and 40 million years. The discovery of this tiny, probably arboreal species should spur renewed attempts to find living salamanders on the larger Caribbean islands. (DW)
- August 10, 2015:
To be considered venomous, not just poisonous, a delivery system needs to be present. Recently Jared et al (2015) showed in two Brazilian frogs a novel method for venom delivery- through spikes in their heads. Corythomantis greeningi and Aparasphenodon brunoi both have bony spikes around the margin of the skull which can pierce through a bed of poison glands, thus injecting the venom into their would-be molesters. They have unusually flexible necks allowing them to twist and turn their heads. Further, they determined that the secretions were 2 times and 25 times more lethal than pitviper venom, respectively. While C. greeningi was able to deliver more venom, A. brunoi has a more lethal venom. (MK)
- August 3, 2015:
A moratorium on US salamander imports may be the best defense against the deadly salamander chytrid fungus, Batrachochytrium salamandrivorans (Bsal), which is poised to devastate North American amphibian biodiversity. Yet, the US Fish & Wildlife Service has not acted to ban imports of salamanders to the US, pending more studies of this recently discovered pathogen. A growing chorus of advocates for a temporary ban includes a new Science Policy Forum report (Yap et al 2015), which presents a salamander vulnerability model showing the regions most at stake. Specifically, the centers of salamander biodiversity in the Southeast US, Appalachia and the Pacific Northwest would be most impacted should Bsal enter one of the adjacent traffic ports. Prevention in this case is the best defense, as we have learned from the wave of extirpations caused by Bd, sister taxon of Bsal. AmphibiaWeb urges immediate action by responsible agencies. (MK)
- July 27, 2015:
It’s a busy time for AmphibiaWeb. Recently, the Society for the Study of Amphibians and Reptiles (SSAR) released their new online, searchable North American Standard English and Scientific Names Database, where you will find links to AmphibiaWeb species accounts for each amphibian. At the SSAR annual meeting at the University of Kansas (July 30th to August 3rd), Michelle Koo of AmphibiaWeb will present a vulnerability model of the alarming Batrachochytrium salamandrivorans (a.k.a. Bsal, the recently described chytrid fungus infecting salamanders) and our proposal for a centralized database to help combat Bsal and Batrachochytrium dendrobatidis (Bd). In fact, more than half of the AmphibiaWeb steering committee will be present, so come find our presentations. We look forward to connecting with you there!
- July 20, 2015:
In a rare example of publishing negative results, Labisko et al. (2015) recently reported the failure to detect Batrachochytrium dendrobatidis (Bd), the culprit behind the often fatal amphibian chytrid infection, in six of eleven amphibian-inhabited Seychelles Islands. These findings included 10 of the country’s 12 amphibian species, which include both frogs and caecilians; 11 are considered globally significant. These findings are particularly important because the climate, habitat, and introduction of Bd susceptible amphibians provide a high potential for Bd to gain a foothold in Seychelles amphibians. The authors recommend the effective implementation of the Seychelles Biosecurity Act and continued monitoring to ensure these unique amphibians stay protected. (AChang)
- July 13, 2015:
Amphibians display a wide spectrum of parental care, from abandonment after laying eggs to provisioning larvae with food after hatching or live birth, which has been considered a fixed trait. Ringler et al. (2015) tested that assumption in Allobates femoralis, a species in which males obligatorily transport tadpoles and females provide no parental care, after observing a small proportion of tadpole transportation by females over a 5 year period. Using both previously observed data and laboratory experiments with wild-caught individuals, the authors tested if mothers transported tadpoles when fathers disappeared. The statistical and experimental results consistently showed that females compensate for unavailable males by transporting tadpoles themselves. The authors urge more cautious labelling of uniparental care as it may oversimplify parental behavioral flexibility. (AChang)
- July 6, 2015:
Rana cascadae is in decline throughout much of its range, and its near extirpation in the Lassen Peak region of northern California was an early example of the general decline of amphibians that has attracted so much attention. Piovia-Scott et al. (2015) studied the relationship of the chytrid pathogen (Batrachochytrium dendrobatidis, Bd) to juvenile survival of R. cascadae in a lake in the Klamath Mts., and a lake in the southern Cascade Mts., northern California. In the first site, Section Line Lake, abundance of juvenile frogs declined by more than 99% between 2009 and 2012, and Bd was 5 times as prevalent as in the other lake, where no clear trend was recorded. Lab experiments showed that the virulence of Section Line Lake Bd was much greater than isolates from other lakes, and genomic differences were also recorded. The study highlights the significance of local processes in host-pathogen dynamics and population declines. (DW)
- June 29, 2015:
The Pine Barrens Treefrog Hyla andersonii is an enigmatic species that exists in three distinct geographic regions: southern New Jersey, the piedmont region of the central Carolinas, and the panhandle of Florida and immediately adjacent Alabama. Warwick et al. (2015) evaluated the species using bioacoustics, microsatellites and morphology, with the goal of making recommendations concerning taxonomy with special consideration for conservation. The guiding principle is Templeton’s conception of populations being conspecific if they display exchangeability. Data were strikingly concordant across the entire combined range, an unusual situation that argues in favor of continued recognition of a single taxonomic species. (DW)
- June 22, 2015:
Last Fall, we highlighted the deadly fungal disease, Batrachochytrium salamandrivorans, now also known as Bsal, which is especially lethal to salamanders having been discovered at sites of mass fire salamander die-offs in Europe (NYTimes Oct 2014). Martel et al 2014 reported on the first susceptibility tests of Bsal on salamander species revealing its lethality to North American taxa. Since then, the US amphibian conservation community has not been idle; several studies and petitions have launched. Indeed a call for banning salamander imports to protect North American species has been growing. One such initiative has been the formation of a Disease Task Team by PARC (read one-page brief here). Their first goal is to build a Strategic Action plan towards protecting North American biodiversity and natural heritage from the Bsal threat. (Browse North American salamanders through our lists by country and state).
- June 15, 2015:
The Cane Toad, Rhinella marina, has been evolving rapidly during its 80-year period of range extension across northern Australia. Extensively documented phenotypic changes in morphology, physiology and behavior suggest that the pace of change appears to be accelerating. A newly published study (Rollins et al., 2015) focused on gene expression reported differences across the historic – invasion axis, with substantial up-regulation of many genes, especially those involved in cellular repair and metabolism. Shifts in gene expression may well underlie the rapid evolution associated with range expansion. These findings open new research opportunities. (DW)
- June 8, 2015:
It is difficult to place current extinction rates into an appropriate time perspective, but in a new analysis of vertebrate extinctions, McCallum (2015) reaches shocking conclusions. Using IUCN/SSC Red List data, comparisons were made with 16 different approaches and 288 separate calculations. Vertebrate extinctions (using designations of Vulnerable to Critically Endangered) have exploded since 1980, with losses 71-297 times larger than end-Cretaceous events. If the analysis is extended to Data Deficient species, the magnitude approaches 8900-18,500 times the mass extinction of the past. The speeds and magnitudes are sufficiently great that present occurrences constitute a mass extinction, the sixth in Earth's history. (DW)
- June 1, 2015:
AmphibiaWeb team member David Blackburn, California Academy of Sciences curator, and digital artists at Ex'pression College (Emeryville, CA) produced an animated music video for Caecilian Cotillion. The Wiggly Tendrils wrote this lovely song last year to commemorate the 200th species of caecilian, those legless amphibians with secretive lives.
- May 25, 2015:
Cave-dwelling organisms are usually very different than their close epigean (surface-dwelling) relatives. However, cave and epigean forms are often genetically very similar, suggesting that morphological evolution has accelerated. Salvidio et al. (2015) studied foot morphology in Hydromantes strinatii, an Italian cave salamander that seems to be following in Gollum's footsteps. They showed that in a population in a 70 year-old artificial cave, the size and shape of the feet had diverged from that of an epigean population to resemble a the feet of a natural cave population. The larger feet of cave salamanders are thought to be adaptive, supporting a hypothesis of rapid morphological adaptation in response to a new environment. (DC)
- May 18, 2015:
The loss of a species' genetic distinctness via hybridization is a conservation concern sometimes difficult to identify and further impeded when the organisms are secretive and with low population numbers. Fukumoto et al (2015) recently overcame this problem by using environmental DNA, specifically DNA found in water samples, to determine the extent of hybridization between the threatened native Japanese Giant Salamander, Andrias japonicas, and invasive Chinese Giant Salamander, Andrias davidianus. After surveying 37 sites across the Katsura River Basin in Japan, they found 9 sites with exotic DNA, including sites that traditional methods excluded from having invasives. The authors recommend environmental DNA methods for monitoring the spread of hybridization in conjunction with traditional catch and release methods to provide a fuller picture for conservation efforts. (AnnC)
- May 11, 2015:
Hyla is a well-known component of the North American frog fauna. However, more than half of the species of Hyla are found in Europe and Asia. Two possible dispersal routes from North America into Eurasia are west-to-east (trans-Atlantic) or east-to-west (Bering Land Bridge). Dispersal over the Bering Land Bridge has generally been assumed, but has not been tested. Using a biogeographic analysis, Li et al. (2015) concluded that Hyla arrived in Eurasia by two dispersals over Beringia, at 20 mya and at 35 mya. Interestingly, although European Hyla are geographically closer to eastern North American Hyla, they are more closely related to East Asian Hyla. (DC)
- May 4, 2015:
Every herpetology student learns that all salamanders are carnivorous at all life stages, unlike frogs in which the larvae may eat a variety of foods and at least one species eats fruit as an adult. Recent work by Hill, Mendelson and Stabile (2015) has shown that a few species of sirenid salamanders willfully ingest vegetation even in the absence of animal prey associated with it. A literature review indicated that the phenomenon had been noticed during examination of stomach contents, but the observations were variously interpreted as ingestion incidental to foraging for animal prey. Future work on the digestive physiology and microbiota of sirenids is warranted. (Joseph Mendelson)
- April 27,2015:
Elizabeth Kolbert has won the Pulitzer Prize in general nonfiction for her book "The Sixth Extinction: An Unnatural History" in which she travels the world describing mass extinction events, untangling causes, and trying to understand solutions. From her publishers: "The Sixth Extinction" began as a New Yorker article of the same title, about crashing amphibian populations in Panama, and parts of the book were published in the magazine in the two-part series "The Lost World." The award is a recognition of both the power of Kolbert's writing and the urgency of her subject. As the subheading on her magazine piece read, "This time, the cataclysm is us." (VV)
- April 20, 2015:
Phenotypic plasticity in frogs has been known to range from variation in coloration to presence of spines and glands associated with reproduction. However, variation in skin texture has long been assumed to be limited, so much that the number, shape and distribution of tubercles, warts, and other skin protuberances is routinely used as a diagnostic character in species descriptions. The effectiveness of skin texture as a diagnostic trait is now questioned by Guayasamin et al. (2015)'s findings that in two species of the large frog genus Pristimantis, individuals have the ability to quickly change skin texture from tuberculate to nearly smooth. In addition to cautioning about the peril of ignoring intraspecific phenotypic plasticity when making taxonomic decisions, the new study hints at some intriguing hypotheses regarding the benefit of skin change abilities in these Andean frogs. (A slideshow of P. mutabilis remarkable ability.) (ACatenazzi)
- April 13, 2015:
In addition to humans, amphibians also benefit from probiotics. Certain bacteria in the skin microbiome of frogs inhibit infection by the chytrid fungus Bd, a deadly skin pathogen. This suggests a strategy that would allow re-introduction of a virtually extinct species, but, the solution is not simple. Becker et al. (2015) showed that in the Panamanian Golden Frog (Atelopus zeteki), application of bacteria that inhibit Bd in vitro did not do so in vivo. Survival was correlated instead with the composition of the pre-existing skin bacterial community. This indicates that information about the skin microbiome in natural populations may be useful for predicting susceptibility. (DC)
- April 6, 2015:
The fungal pathogen, Batrachochytrium dendrobatidis (Bd) , which causes chytrid infections in amphibians, is known to have varied effects on different species. In some, infection is lethal, while other species appear to be only carriers. Susceptibility to the fungus is thought to be related to the evolutionary history of exposure to Bd. Talley et al. (2015) recently investigated the historic presence of Bd in Illinois, where field surveys showed Bd presence without amphibian die-offs. Their testing of museum specimens of 10 species collected between 1888 and 1989 revealed that Bd occurred in four species (at rates between >7% and 38.3%, with the highest historic prevalence in Rana sphenocephala) from over 120 years ago. Their findings illustrate why evolutionary history is important for effective conservation management plans. (AChang)
- March 30, 2015:
Identifying the effects of climate change on amphibians is important. Connette et al. (2015) warns that ecological factors or systemic bias may be causing apparent body size changes that are attributed to climate change. Connette and colleagues conducted visual encounter surveys of Plethodon metcalfi and measured the body size (SVL) and other parameters of encountered animals for two years and conducted mark-recapture surveys of P. shermani for six years, all in southwest North Carolina. They found that large adult P. metcalfi were disproportionately represented in samples collected shortly after rainfall and towards the middle of the summer active season, with an 11% difference in mean body size across survey conditions. There was large annual variation in P. shermani in all age classes, with larger individuals more likely to be detected after rainfall. Connette et al. (2015) cautions that plethodontid salamanders may be particularly susceptible due to their fossorial nature and sensitivity to high temperatures and moisture, and this could easily lead to biases in surveys. Large multi-year, multi-season surveys are needed for accurate measures of population and body size variation. It is possible that climate change has resulted in selection for smaller adult body size in recent decades, but other factors, such as weather conditions, yearly variation, and exposure during sampling, can also affect findings. (CS)
- March 23, 2015:
As humans have changed global biodiversity, infectious diseases have risen and wildlife managers are faced with the question of how to respond to parasite transmission. Rohr et al. (2015) address this issue by conducting wetland surveys, predators-parasite foraging experiments, testing the effect of different predators on parasitic trematode cercariae infection rates of tadpoles, and mathematically modeling infection rates in frogs. They found that predator diversity decreased infection rate and that predators that feed on parasites but not the focal host (non-intraguild predators), were especially effective at keeping trematode densities down. The authors encourage the cooperation between the fields of biocontrol and disease management and further believe that identifying more traits in predators and hosts may help us combat parasite infections. (AChang)
- March 16, 2015:
Until now, only one genome from the >7,300 species of amphibians has been sequenced. Now, a group led by Chinese researchers from the Kunming Institute of Zoology (Sun et al 2015 PNAS) have produced the second, from Nanorana parkeri, a frog endemic to the Qinghai-Tibetan Plateau. Its genome encodes about 20,000 genes, a number similar to that of humans. At 2.3 gigabases, it is larger than that of Xenopus tropicalis, which is 1.5 gigabases. The addition of a second amphibian genome provides a new source of reference data that is crucial to our continued exploration of the evolutionary connections between genotype and phenotype. (DC)
- March 9, 2015:
Arkive’s Conservation Heroes series highlights Alessandro Catenazzi, one of AmphibiaWeb’s steering committee members, and we thought it would be a good idea to do so, too! Alessandro joined AmphibiaWeb in 2012 when he started working with our co-founder and Associate Director Vance Vredenburg. Further, he is a reviewer for the Red List Assessments. Since then, Alessandro has built an impressive record of research on Peruvian herpetology, chytrid disease, and new amphibian species discoveries. So far, Alessandro and his colleagues have described 16 new species of amphibians including Telmatobius ventriflavum, which he recounts the story of its discovery in the Arkive profile. (MK)
- March 2, 2015:
Scientists have documented that in response to climate change, the timing of breeding has shifting to earlier for many species, but the consequences of this phenomenon for populations and individuals are not known. Bernard (2014) investigated breeding and fecundity in six southern wood frog (Rana sylvatica) populations, and found that warmer winters were associated with earlier breeding in this species, and lower fecundity for females. Earlier breeding also led to slower larval development and changed the timing of metamorphosis to earlier. Changes in the amount of precipitation was not associated with earlier breeding but was correlated with female fecundity. The lower fecundity rates could be caused by increased energy consumption during the warmer winters, but it was unclear why changes in winter precipitation rates were correlated with frog fecundity. (CS)
- February 23, 2015:
In disease dynamics, the "dilution effect" predicts more diverse host communities will have decreased incidence of disease through a complex mix of host-interactions and habitat partitioning. Becker et al (2014) tested this effect of host diversity on chytridiomycosis dynamics with experiments on amphibian host species with a range of traits, from terrestrial to aquatic to arboreal and from multi-species to single species communities. They report that high host diversity led to reduced Bd infection by as much as 66.5 percent less than single species. However, ecological interactions are complex: while one frog, Brachycephalus pitanga, had decreased Bd infections in diverse environments, it may be causing its aquatic neighbors an increase in Bd infections through habitat displacement. (MK)
- February 16, 2015: Here's a follow up on our January 5th Amphibian News on Limnonectes larvaepartus, the new frog species that was found to give birth to tadpoles. Kusrini et al. (2014) examined the bodies of gravid females to further elaborate on this mode of reproduction. They found several tadpoles being held in the translucent oviduct, which completely filled the abdominal space. Upon closer study, they saw some tadpoles had yolk reserves attached to them, though there were no feces within the oviduct. Scientists determined that the tadpoles feed from yolk reserves rather than the oviduct wall. The yolk reserves are sufficient to last them until birth, at which point they rely on an external food source. Dark particles found in the guts of the unborn tadpoles suggest that they may also consume feces and dead tadpoles before birth. (Gordon Lau)
- February 9, 2015:
Some brightly colored newts, such as Taricha, are defended from predators by tetrodotoxin (TTX) in their skin. But how do newts protect themselves from TTX, a deadly toxin? It is known that genetic changes in the genes for sodium ion channels provide the newts with resistance against TTX. Hanifin and Gilly (Evolution 2015) demonstrated that these same genetic mutations are present even in some newts that do not have TTX, such as Pleurodeles. More importantly, these changes confer on Pleurodeles a small amount of resistance to TTX. This may have opened an evolutionary path to acquisition of TTX as a defense in newts such as Taricha. (DC)
- February 2, 2015:
An important issue in climate change and its relation to disease incidence is acclimation to shifts in temperature and precipitation. Raffel et al (PRSB 2015) support results of earlier work showing that temperature increases could exacerbate the effects of chytridiomycosis because of lags in host acclimation. Chytrid growth was greater in Notophthalamus viridescens following a shift to a new temperature, relative to newts acclimated to that temperature. But there are also important interactions with other factors (e.g., precipitation, soil moisture) that need to be taken into account. The study highlights the complex interaction between environmental effects and disease dynamics. (DW)
- January 26, 2015:
Although navigation has been studied in many vertebrates, very little is known about amphibian spatial learning abilities. Pasukonis et al. (2014) studied homing in territorial male Allobates femoralis, a species that shuttle their tadpoles, to determine if experience played a role in spatial orientation. Using a harmonic direction finder, they found that animals translocated within a familiar range were able to use direct paths back to their territories while animals translocated to unfamiliar surroundings of similar distances, were unable to orient toward their territory. The authors concluded that poison dart frogs use spatial learning for navigation within a local area. (AChang)
- January 19, 2015: Xenopus laevis, the African Clawed Frog, is the most studied species of frog. Google Scholar yields 19,700 hits in the last five years alone. Ironically, even an experienced field herpetologist would have trouble determining whether a frog was in fact Xenopus laevis. Furman et al. (2015) have solved this problem. Using a multigene analysis of ~183 individuals from throughout Sub-Saharan Africa, they demonstrated that specimens identified as X. laevis in fact represents four species (laevis, petersi, victorianus, and poweri), each restricted to a major geographic region of Africa. Although these names already existed in the literature, they were often applied to the wrong species. (DC)
- January 12, 2015:
As with humans, noise affects the sound transmission of frog calls. Flowing water is a common source of background noise, and stream frogs tend to call at a higher pitch, but why is this? The species may have evolved a higher-pitch call for more efficient transmission--the acoustic adaptation hypothesis. Or, species that call at a less-than-optimal pitch are "filtered out" by the habitat and don't call near streams - the habitat filtering hypothesis. Vargas-Salinas and Amézquita (2014) compiled data on 110 species of stream- and non-stream frogs. After correcting for phylogenetic relationships and body size (larger frogs on average make lower-pitched calls than smaller ones), they found that the habitat filtering hypothesis was better supported than the acoustic adaptation hypothesis. Thus, ecology, more than evolution, explains why stream frogs call at a higher pitch. (DC)
- January 5, 2015:
Amphibians display an amazing array of life histories. In a paper describing a new species of dicroglossid frog from Sulawesi, Limnonectes larvaepartus, Iskander et al. (2014) also describe a new life history specialization. As far as yet known, this is the only frog to give birth to tadpoles. Oviductal tadpoles are well provisioned with yolk and reach at least Gosner stage 35 prior to parturition. The tadpole seen above, at ca. stage 25, was released by a female on capture. There is no evidence that metamorphosis occurs in the oviduct. (DW)
- Happy Holiday season from AmphibiaWeb. 2014 has been a very active year for us, with initiation of new sections: Educational Resources, Stories from the Field, and new efforts underway to develop Life History Trait database and Amphibian Diseases portal. More than 2,200 photos have been added to a database containing about 31,000 photos. During the year, 167 species have been added and our monthly literature listing continues its steady growth and timely production. If you enjoy AmphibiaWeb, help spread the word by visiting our Zazzle AmphibiaWeb Shop and consider making a donation to help ensure our continued success.
- December 22, 2014: While Rana cascadae is relatively widespread in montane habitats from northern California into southern British Columbia, declines attributed to the chytrid fungus Batrachochytrium dendrobatidis (Bd) seem focused in northern California populations. Piovia-Smith et al. (2014) studied virulence of Bd from two lakes suffering declines, finding that isolates from the midst of the decline were more virulent and induced greater mortality of juvenile frogs than Bd isolated from a more stable frog population. In genomic studies, the virulent isolate showed unusual features, including chromosomal differences. Specific genomic and phenotypic features of Bd might account for differences in mortality within species from different localities, as well as differences among species of amphibians. (DW)
- December 15, 2014:
An emerging biodiversity pattern is that of single amphibian species that are widespread across a continent are found to be complexes of several cryptic species. These are species that are difficult to distinguish without detailed analysis of traits such as mating calls in frogs or DNA sequences. An extreme example is the species Dendropsophus minutus, which extends over several ecoregions, including the wet forests of Amazonia, the coastal Atlantic forests of southeastern Brazil, to dry scrub forest of the Caatinga. Depending on the region, the species occurs from sea level to 2000 m asl. Using mtDNA sequences, Gehara and 29 co-authors (2014) identified 43 lineages within the single "species," many of which may represent cryptic species. The distribution of one lineage covers more than 1 million km2. This broad-brush study is an important first step in documenting taxonomic and biogeographic patterns of Neotropical biodiversity that beg for deeper analysis. (DC)
- December 8, 2014:
Two phylogenetically-related, highly virulent CMTV (Common Midwife Toad Virus)-like ranaviruses have caused mass mortality in multiple, diverse amphibian species (3 salamanders, 3 frogs) in a national park in northern Spain. A new paper by Price et al. (2014) documents these amphibian host populations and follows them in real-time following emergence of these Ranavirus. Multiple amphibian species experienced mass mortality and population collapse at multiple sites in the region. This is striking example of a novel, generalist pathogen repeatedly crossing the species barrier with catastrophic consequences in an amphibian community and is the result of the introduction of a single viral strain. (CS)
- December 1, 2014:
While we have known some animals can sequester diet-based defensive compounds to pass onto their offsprings, it has rarely been shown that there is a behavioural component, such as a preference for such prey by these species. Kojima and Mori (2014) addressed this behaviour in the venomous Tiger Keel-backed snake (Rhabdophis tigrinus) which preys on many amphibians including the robust, toxic Japanese Common Toad (Bufo japonicus). In their field work and prey-preference experiments, they showed that female snakes preferentially sought out the more scarce toads when gravid versus behaviour in males or non-gravid females. By seeking out toads, these females are able to confer more defensive compounds to their offsprings, which may be an effective antipredator defense as well as a rich example of ecological interdependence. (MK)
- November 24, 2014:
Because of their biphasic life history, many amphibians regularly cross roads, where they risk death from vehicles. Under-road tunnels have been used to mitigate traffic mortality with varied success. However, experimental testing for tunnel efficiency has been limited. Hamer et al. (2014) tested tunnel efficiency using two species of Australian bell frogs (Litoria aurea and L. latopalmata) and one species of marsh frog (Limnodynastes peronii), which have very different reproductive seasons than frogs in the Northern Hemisphere. They found far fewer Australian frogs entered and crossed tunnels than northern hemisphere species. The authors attributed this difference to life history and recommend more testing of installed under-road tunnels to determine their efficiency in conservation. (AChang)
- November 17, 2014:
Who would have predicted that an undescribed, cryptic species of frog would be uncovered within New York City? In a recent paper Feinberg and associates describe Rana kauffeldi, a species previously confused with R. pipiens and R. sphenocephala. The new species has a narrow lowland distribution, from northern New Jersey to central Connecticut (including Staten Island), but based on calls, it might occur as far south as North Carolina. Its unique call gave it away, and genetic data confirmed its distinctiveness. (Listen to its call here.) Discoveries such as this are critical in efforts to preserve biodiversity, because few sites remain in the heavily urban area the species occupies. (DW)
- November 10, 2014:
Cryptic diversity takes on special focus in the past month when two newly described species were reported from well known city regions from opposite sides of the globe: New York City and Kochi, India. Using multiple lines of evidence, Feinberg et al 2014 formally described a new leopard frog (Rana kauffeldi) from New York City which ranges along the coast from Connecticut to New Jersey. In Kochi, India, another urban center, Biju et al (2014) described a new Golden-backed frog, Hylarana urbis, along with 6 other Hylarana species also with molecular and morphological data. Both these frogs highlight the dangers of cryptic diversity where new species are discovered already threatened with habitat fragmentation and habitat loss of unmeasurable degree.
- November 3, 2014:
Last year the second chytrid pathogenic for amphibians was discovered in western
Europe. Batrachochytrium salamandrivorans (also known as Bs) seems to be more virulent than its congener. A new study tests susceptibility of salamanders and some other amphibians (Martel et al Science 2014). Exposure of 35 species (24 salamanders) to 5,000 zoospores for 24 hours resulted in most salamanders but no frogs or caecilians becoming infected. 41 of 44 specimens of Palearctic salamanders died, including 5 salamandrid and 1 plethodontid species. Bs was lethal to both North American salamandrids. Bs was present but tolerated by most east Asian salamandrids tested, leading the authors to suggest that Bs spread to Europe from Asia via the pet trade. (DW)
- October 27, 2014:
Carlos R. Vasquez-Almazan, Curator in the Museo de Historia Natural in Guatemala City, is the winner of the Sabin Award in Amphibian Conservation, administered by the Amphibian Survival Alliance this year. This award recognizes the impressive efforts of Carlos in guiding the establishment of new conservation areas in Guatemala, especially the Sierra Caral Reserve. Carlos is an active systematist and ecologist, with a strong focus of amphibian conservation. We show a video presentation of Carlos accepting the Whitley Award from the Whitley Fund for Nature in 2012. AmphibiaWeb congratulates Carlos Vasquez-Almazan for his outstanding efforts in conservation, now recognized by two major awards.
- October 20, 2014:
In a recent issue of Nature Communications, Twomey et al. (2014) report on the Peruvian frog Ranitomeya imitator, which has evolved to mimic locally abundant poisonous frog species. In a classic case of Mullerian mimicry, individuals of R. imitator vary dramatically across a narrow transition zone in which they closely mimic R. fantastica versus R. variabilis. Microsatellite markers reveal restricted gene flow across this transition zone, perfectly matching the transition of R. imitator morphological characters and mating calls. Mate choice experiments revealed strong evidence for assortative mating in R. imitator within the transition zone, proving that genetic isolation can arise from natural selection for mimicry. While it is unclear if these reproductive barriers arose through sympatry or allopatry, the results clearly point to a role for mimicry in the speciation process. (AZink)
- October 13, 2014:
The 10th Latin American Congress of Herpetology will be held in Cartagena, Colombia from November 30 to December 5th, concomitantly with the 4th Colombian Congress of Zoology. The rich scientific program includes nine herpetological symposia, seven of which will present novel findings on Central and South American amphibians. These symposia will cover biogeography, bioacoustics and communication, parental care, ecological physiology, effects of climate change, and conservation. Regular sessions will include evolutionary ecology, physiology, systematic, diversity, conservation, reproductive biology and behavior. Also in the program is a workshop on amphibian parasitology. With 775 presentations scheduled for the event, this meeting will be the largest gathering of herpetologists working in Latin America.
- October 6, 2014:
Pathogenicity in different organisms is known to decline through time in many instances. Voyles et al. (Ecol Evol 2014) conducted experimental studies of two cryo-archived isolates representing two lineages of the amphibian pathogen Batrachochytrium dendrobatidis (Bd). The results support earlier suggestions that Bd may evolve rapidly but also challenge the widely held view that pathogenicity will always attenuate, especially in the lab. This study is an important contribution for understanding the evolution of virulence in this pathogen.
- September 29, 2014:
The link between microbial communities and individual health is gaining strength in recent years. For amphibians, evidence grows that certain microbes may provide resistance to the fungal pathogen Batrachochytrium dendrobatidis which causes Chytridiomycosis, a leading cause of amphibian decline. Becker et al. (2014), investigated the differences in microbial communities in wild vs. first generation captive-reared Panamanian golden frogs (Atelopus zeteki); reintroductions of captive reared golden frogs has been one method of combating Chytridiomycosis. Although both wild and captive-reared frogs shared 70% of the microbial species, the authors also found significant differences in microbial diversity and richness with captive reared frogs, having on average more species bacteria, and wild animals having communities with greater evenness and phylogenetic diversity. The effects of these microbial differences are currently unknown, but could lead to the failure of reintroductions due to loss of immunity to local pathogens. Thus the authors argue for reducing time spent in captivity or raising captive-reared animals with non-pathogenic native substrates. (AChang)
- September 22, 2014:
Amphibian biologists studying chytrid infections in the tropics have found the highest prevalence in mid-elevation wet forests. Hotter lowland sites, especially in drier habitats, have experienced less impact of chytrid. Zumbado-Ulate et al. (EcoHealth, 2014) studied frogs at two sites in dry, lowland forest in northwestern Costa Rica, finding extremely low prevalence, and in those few frogs infected, low genetic equivalents. Such habitats serve as climatic refugia from chytrid, highlighting the importance of tropical dry forest conservation for preservation of species, such as Craugastor ranoides. (DW)
- September 15, 2014:
For the first time, the auditory apparatus of caecilians, a largely fossorial group of amphibians, has been examined and compared with other animals. Maddin and Sherratt (2014) hypothesized that caecilians would have similar inner ear morphology, specifically semicircular canals that morphologically maximise sensitivity to correspondingly complex physical stimuli, as other animals which demonstrate spatially complex behaviours like living underground and navigating in three-dimensions. Their comparative work within amphibians showed that caecilians and frogs had similar curved canals but caecilians had the largest sacculus of all amphibians (enlarged saccule had been noted in earlier studies) among other differences. Taken together, the inner ear of caecilians shows unique adaptations to fossoriality like increased sensitivity to ground-borne vibrations and degeneration of airborne sound detection. (MK)
- September 8, 2014:
A new study (Pyron 2014, Systematic Biology) of the biogeographic history of amphibians is based on an updated chronogram of 3309 species (about 45%) and existing molecular data. The Pangean origin and subsequent fragmentation into Laurasian (salamanders) and Gondwanan (frogs, caecilians) segments led to occupation of 12 global ecoregions. Earth history (fragmentation and connection) accounts for most patterns of dispersion, but there are three well-supported instances that require long-distance oceanic dispersal: pelodryadine frogs dispersing to Austroasia from South America, micrixalid frogs dispersing to India from likely African origins, and hyperoliid frogs in Madagascar dispersing from Africa. Numerous questions remain concerning details for specific clades, but the broad patterns of distributional history are fairly clear, and both vicariance and dispersal have been important. (DW)
- September 1, 2014:
Currently, the IUCN lists 24.5% of assessed amphibians as Data Deficient. Using high risk traits of life history, environment and habitat loss, Howard and Bickford (2014) predicted the extinction risk of Data Deficient species and found that they are likely to be more threatened by extinction than assessed species. Their models highlight regions of risk anomalies, areas in Central and South America, West Africa, and the island of New Guinea in particular. Models as these may become important tools for conservation assessment when species are deemed Data Deficient, or worse, have not been assessed as in the case of over 1,000 species of amphibians. (MK)
- August 24, 2014:
Tadpoles of tropical frogs show great diversity of anatomy and life history. A recent publication (Oberhummer et al., Zootaxa 2014) describes tadpoles of two megophryid frogs from Borneo (Leptobrachella brevicrus and Leptolalax dringi) that are highly specialized for living fossorially, in gravel beds of small, montane streams. Tadpoles of both species have an elongated, worm-like shape including a long tail and very small eyes. Knowledge of the diversity of tadpoles continues to grow as biologists spend more time in areas of amphibian megadiversity. (DW)
- August 18, 2014:
Peru is well known for its diversity of frogs, yet there have been relatively few field studies of glassfrogs (Centrolenidae) in this country. Research to date indicates that glassfrog diversity is higher in the northern Andes. However, a new study by Twomey et al. (Zootaxa 2014) suggests that part of this trend may be due to sampling effort. The authors conducted fieldwork in Peru, and using an integrative approach to taxonomy they uncovered 4 new species from the family. Their results indicate that more exploration in the Peruvian Andes will likely uncover a higher diversity of glassfrogs than that currently recognized.
- August 11, 2014:
Mountain Yellow-legged Frogs (Rana muscosa and R. sierrae) were once the most commonly encountered vertebrates in the Sierra Nevada of California. Despite having 84% of the combined range included in National Forests or National Parks, both species have severely declined, and frogs have disappeared in 93-95% (minimal estimates) of historical sites. A large multi-agency report (linked here) summarizes the situation, supporting endangered status for the taxa and identifying chytrid infection, trout introductions and habitat loss, among other factors, as contributing importantly to the declines. Some potential remediation efforts are underway, and research is on-going. (DW)
- August 4, 2014:
Amphibian Survival Alliance (ASA) has launched a new online magazine aimed at children 7-14 years of age. As with its other quarterly publication, this one is called Frog Log Jr., which we are hosting on our Education section. AmphibiaWeb contributed the first article and looks forward to collaborating with ASA in the future to help educate young people about the importance and conservation of amphibians. Take a break to read it!
- July 28, 2014:
Color can protect frogs in at least two ways: Dull frogs are cryptic against the background and avoid detection, or conspicuous frogs are easy to detect, but usually have a defense so that predators learn to avoid them. It turns out that pattern, not just color, is also important. Qvarnström et al. (2014) show that video images of dull green Strawberry poison frogs, Dendrobates (Oophaga) pumilio, were inconspicuous to predators (chickens) whereas dull green frogs with blotched patterns were as conspicuous to predators as brightly colored frogs. So, a blotched pattern may provide an evolutionary pathway by which the transition between the adaptive phenotypes can be made. Moreover, blotching may constrain the frequency with which this transition can be made. (DC)
- July 21, 2014:
The chytrid fungus Batrachochytrium dendrobatidis (Bd) has had devastating impact on amphibian populations in many parts of the world and its impact continues, threatening efforts at captive breeding and reintroduction of species. A new paper reports that frogs studied can acquire resistance, behavioral, immunological or both. In experiments, McMahon et al. (2014, Nature) show that frogs exposed and infected by Bd, but subjected to temperature clearance, learn to avoid sources of infection. With forced exposure, the number of previous exposures was a negative predictor of reinfection and a positive predictor of lymphocyte proliferation, thus overcoming pathogen-induced immunosuppression. These results suggest several therapeutic approaches to dealing with Bd. (DW)
- July 14, 2014:
Bonne Fête! In honor of Frances quatorze juillet celebrations, we highlight French amphibians. France has 42 amphibian species, and only 3 endemic species which only occur in its island region, Corsica in the Mediterranean Sea: Discoglossus montalentii, Salamandra corsica, Euproctus montanus. Frances most widespread frog species is the Midwife Toad, Alytes obstetricans, which occurs in almost all parts of continental France but is currently in decline. While Alytes is not particularly known for its legs, France recognized the impact their famous cuisine of frog legs has had on their amphibian populations, and in 1980 banned commercial harvesting of native frogs. Cuisses de grenouilles dishes served in France now depend on Asian imports of frogs. Many western European amphibians have core parts of their ranges in France, making it an important biogeographic crossroad. (MK)
- July 7, 2014:
Some amphibians appear to be genuinely rare, making it very difficult to assess their conservation status. A new paper (Batista et al. 2014 Zootaxa) describes two new species of Ecnomiohyla from Panama. Each is represented only by the unique holotype. Frogs of this genus are known as Fringe-limbed Treefrogs. They are large, spend their lives in forest canopies (laying eggs in bromeliads), and are generally encountered only by chance. (DW)
- June 30, 2014:
Everyone knows that frogs have sticky tongues. But, how sticky? A newly published study (Kleintiech et al. 2014) reports on direct measurements in Ceratophrys sp. (likely laboratory bred hybrids). Tongue adhesive forces were found to be very great, so great that they are beyond the body weight of even the very large (for frogs) species of this genus, and more than any potential prey. Adhesion is strong even in parts of the tongue that have low mucous levels, suggesting that surface profiles and material properties are likely to be important factors. Frog tongues are analogous to pressure sensitive adhesives, such as tapes. Sticky indeed! (DW)
- June 23, 2014:
Although many Neotropical salamanders are intimately associated with bromeliads, we know very little about which characteristics salamanders use to select bromeliads. In a study of two sympatric species on Volcán Pacaya, Bolitoglossa morio and B. pacaya, Ruano-Fajardo et al. (2014) report that the two species were found in the same bromeliads across several sites and selected bromeliads with similar characteristics. Salamanders were found more often in larger, cooler bromeliads, and larger salamanders were found in bigger bromeliads with higher pH. Large, cool bromeliads may provide an important buffer for salamanders against adverse environmental conditions, especially in harsh environments such as on the active Pacaya volcano. The fact that the two species selected similar bromeliads means that they probably partition some other element of niche space besides microhabitat. (Sean Rovito)
- June 16, 2014:
For some time biologists have known that amphibians in arctic environments survive the long winters by essentially freezing. A new paper (Larson et al. 2014) reports on tracking Rana (Lithobates) sylvatica in interior Alaska to their overwintering sites and monitoring subsequent activity. The frogs stayed frozen on average for 193 consecutive days, experiencing average minimum temperatures of -14.6°C, with 100% survival. Very high cryoprotectant (glucose, glycolipid) levels in the frog are hypothesized to enhance freeze tolerance. (DW)
- June 9, 2014:
The user-friendly field guide The Amphibians and Reptiles of Mindo, recently published by Alejandro Arteaga, Lucas Bustamante, and Juan Guayasamin, presents data on 101 species found in Mindo, northern Ecuador. Designed for students, naturalists, tourists, and scientists alike, each species account includes a list of field-diagnostic characters, natural history notes, outstanding photographs, and a map of the species distribution in Ecuador. A list of expert reviewers and a note on each species conservation status is also included. Twelve species found at Mindo have been described in the last 10 years, and additional species records are expected. Although no lungless salamanders have been recorded at Mindo, two species, Bolitoglossa biseriata and B. chica, occur nearby (10 and 18 km away, respectively). This book will stimulate further studies and promote the conservation of the diverse amphibian and reptile fauna in Ecuador. You can purchase or read online now. (RvM)
- May 26. 2014:
The Sixth Conference on the Biology of Plethodontid Salamanders was held in Tulsa, Oklahoma, May 18-20, 2014. The honoree was Steve Tilley, co-organizer of the first conference in 1972 and one of only a few to attend all conferences. Steve presented the Keynote Address, on geographic variation in Desmognathus and problems related to species delimitation. Speakers were mainly from the USA, but talks were given by scientists from China, France and Mexico. Both talks and posters featured many presentations by students. Following the conference, informal field trips in far eastern Oklahoma uncovered four species of Eurycea and one Plethodon.
- May 19, 2014:
The National Congress in Guatemala, in an overwhelming vote on May 13, approved establishment of the Sierra Caral as a National Protected Area. Within the reserve, on the Honduras border, is the Amphibian Conservation Reserve (ACR, Reserva La Firmeza), purchased with the support of many international organizations working with Guatemalan biologists. The new reserve covers 18,000 hectares and the ACR is 2,300 hectares, one of the richest areas for amphibians in Central America, with at least 35 species, many of which are endangered or critically endangered. This is a major victory in the on-going effort to conserve amphibians in Middle America.
- May 12, 2014:
While recent work has led to new understandings of the relationships of the frog family Brevicipitidae to other frog families, there has been little attention to the evolutionary history within these African frogs. Loader et al. (2014) provide a detailed phylogeny for the many brevicipitids in the eastern Afromontane region extending from Zimbabwe north to the Ethiopian Highlands. They find strong evidence that this family of rotund, sexually dimorphic frogs originated in the early Cenozoic, and suggest that the high diversity of phylogenetically important lineages in the Eastern Arc Mountains is the result of long-term forest stability. (DB)
- May 5, 2014:
Males of many glassfrogs provide parental care to their eggs. Recent research by Delia et al. (2014) found that Hyalinobatrachium fleischmanni embryos can time hatching to cope with delinquent dads. In this species, there is a lot of variation among fatherssome provide care for up to 19 days, while others abandon clutches as early as three. Working at a site in Sierra Madre del Sur of Oaxaca in Mexico, Delia et al. conducted a male removal experiment to determine if embryos hatch once abandoned. They found embryos hatched several days early when abandoned, but extended development when fathers continued providing care. (Jesse Delia)
- April 28, 2014:
Tadpoles of Occidozyga species have been reported to be carnivorous, preying on insects, even other tadpoles. Haas et al (2014) show new photographic evidence for the previously undocumented larval feeding behavior in O. baluensis, the Bornean Seep Frog, and detailed descriptions of the anatomical adaptations to accommodate their carnivory. They hypothesize that suction feeding is used for gaining prey, and that they are obligate carnivores. (MK)
- April 21, 2014:
The 200th species of caecilian is Ichthyophis multicolor from Myanmar (Wilkinson et al., 2014, Zootaxa 3785 (1) ). To commemorate this event, the California Academy of Sciences and AmphibiaWeb present a celebratory song, Caecilian Cotillion! Enjoy!
- April 14, 2014:
In 1983 Narins and Smith found that body size and call characteristics in males of the Puerto Rican species Eleutherodactylus coqui (the coqui frog) vary with elevation. At warmer, lower elevations, frogs were small, with short, high-pitched calls, whereas high-elevation frogs were larger, with longer and lower-pitched calls. Fast-forward 23 years to 2006: Narins and Meenderink (2014) re-studied the same sites; at a given altitude, today's frogs are smaller with shorter and higher-pitched calls. Based on temperature data, they suggest that these changes in calls result from a warming climate that has displaced populations to higher, cooler regions. If these climatic changes continue, male calls may change to the point where females no longer recognize and respond to the mating calls, which likely would be detrimental to mating success and its survival. (DC)
- April 7, 2014:
Woodland Salamanders (Plethodon) in the southern Appalachian Mountains of the US have responded to climate change by shrinking. Caruso et al. (2014) studied 102 populations of 15 species and found that six Plethodon species have shrunk significantly in overall body size over a 55 year period. The proximal reason is that modeling shows that metabolic rate has likely increased by 7 8%, leading to the plastic response in phenotype. Amphibians appear to be more susceptible to environmental change than previously thought, and the current plastic response might well lead to an adaptive response. (DW)
- March 31, 2014:
The presence of predators are known to induce morphological changes in amphibian larvae, a form of phenotypic plasticity. What happens when tadpoles are exposed to introduced predators, like the Red Swamp crayfish, a common invasive species? Nunes et al (2014) experimented with nine Portuguese species (including Iberian endemic Discoglossus galganoi), exposing tadpoles to dragonfly larvae or crayfish cues to measure induced phenotypic plasticity and almost all (8 of 9) altered their morphology with the native predator but only four did in the presence of the non-native crayfish. The variation in responses, including changes in timing of metamorphosis, obviously have implications in predicting the impact of invasive species on amphibian declines. (MK)
- March 24, 2014:
Increasingly Google is the first reference source if you are seeking a comprehensive list of all amphibians in North America. As online information becomes more ubiquitous, the availability of books containing the same information have declined. Countering this trend, herpetologists M. J. Fouquette and A. Dubois have released "A Checklist of North American Amphibians and Reptiles (vol. 1 Amphibians; reptiles will follow, published by Xlibris, 2014). Fouquette and Dubois' book is not a re-hash of existing information, but includes several novel uses of scientific names, especially of subspecies. A "checklist" is a technical reference that treats all species and subspecies of a taxon in a particular geographic region. It includes extensive information on taxonomy and distribution. The classical checklist is much more than a list. Despite the obvious importance of these, no checklist of the species and subspecies of amphibians of North America, either online or in print, has been available since the "Checklist of North American Amphibians and Reptiles" by K. P. Schmidt in 1953. (DC)
- March 17,2014:
The Rio Frio Salamander, Ambystoma leorae, long ago disappeared from the heavily polluted stream where it was first found. The stream-adapted species, once included in the no longer recognized genus Rhyacosiredon, has been rediscovered (the last report of a surviving population is 1983). A new study (Sunny et al. 2014) reports that the critically endangered species persists in a single population on Volcan Tlaloc, located adjacent to what may be the largest and densely urbanized area on earth. The salamanders, found in two small streams, show low genetic diversity but high average heterozygosity, and three genetic subpopulations were recognized in the restricted geographic range. While it is good news that the species persists, its habitat is very restricted and it remains at high risk of extinction. (DW)
- March 10, 2014:
Late in February 2014, a disturbing discovery was made in Ireland. At a lake in the open flatlands of The Curragh, Kildare, up to 300 frogs were found dead. The cause of the mass die-off remains mysterious but the Herpetological Society of Ireland have taken water samples for testing and urge the public to report any other mysterious frog deaths. (More information is posted on their website.) Although the dead and dying frogs, Rana temporaria, are listed as Least Concern by the IUCN, it is only one of three amphibian species known to Ireland and is a protected species in the Republic of Ireland, where populations have declined from mostly habitat alteration. (MK)
- March 3, 2014:
The chytrid fungus (Batrachochytrium dendrobatidis, Bd) is known to have caused amphibian population collapse and extinction in disease outbreaks globally. The origin of Bd is still unknown, but the timing of emergence in different regions is becoming better characterized. Using qPCR, Rodriguez et al. (2013) tested over 2,700 amphibian specimens collected in Brazil from 1890-2010 for the presence of Bd. They found a mean prevalence of Bd infection of 23.9 % over the sampled time period. They report divergent strains of Bd in Brazilian amphibians and suggest that Bd was not introduced to Brazil via the bullfrog trade. The authors conclude that Bd has been enzootic (endemic) in Brazil for over a century. (VV)
- February 24, 2014:
The eastern slopes of the Andes and the neighboring western Amazonian lowlands in South America house remarkable levels of biodiversity. A new survey of Peru's Manu National Park and its buffer zone (Catenazzi et al 2013) found more amphibian and reptile species than in any other protected area worldwide (155 amphibian and 132 reptile species). Their study also highlights the impact that the chytrid outbreak in the upper portions of the protected area (previously reported in Catenazzi et al 2011) have on species richness. The newly reported species list includes seven imperiled amphibian species and two reptile species. Hear from the investigators in a video of AmphibiaWeb's Rudolf Von May and Alessandro Catenazzi. (MK)
- February 17, 2014:
Woodland plethodontid salamanders play major roles in forest ecosystem dynamics. They are the most abundant vertebrates in many forests, and they are such major predators that they depress invertebrate species populations, thus retarding decomposition and enhancing leaf litter retention. This contributes importantly to carbon sequestration. Best and Welsh (in Ecosphere 2014) used experiments to show that a single Ensatina in a 1.5 m2 enclosure increased litter retention by 13.3%. The contribution of salamanders to ecosystem dynamics in general and to carbon sequestration have been under appreciated. (DW)
- February 10, 2014:
Many conservation efforts for amphibians concentrate on protecting aquatic habitats, yet numerous species have both aquatic and terrestrial life stages. A new study used stable isotopes and stomach contents (from both live animals and museum specimens) to analyze food web linkages for the threatened California Red-Legged Frog (
Bishop et al 2013). The stable isotope data found that 99.7% of this frog's diet came from terrestrial prey. Wet and dry season stomach content samples flushed from live frogs had 90% terrestrial prey, and museum specimens contained 82% terrestrial prey. These data suggest that conservation efforts should protect not only aquatic habitats necessary for reproduction (these frogs have aquatic larvae) but also protect surrounding terrestrial habitats, emphasizing the conservation importance of understanding food web linkages between amphibians and their prey, both aquatic and terrestrial. (VV)
- February 3, 2014:
The African microhylid frog Phrynomantis microps lives unharmed in the nests of ants that are normally highly aggressive to intruders. These ant nests provide the frog with a retreat from predators and also a humid environment during the dry season. Rödel and colleagues (2013) studied the skin secretions of this frog and isolated two peptides that inhibit the aggressive behavior of these ants. These peptides seem to interact with the ants chemoreception and disguise the frogs as either a nestmate or, at the least, as not an intruder. (DBlackburn)
- January 27, 2014:
Alexander von Humboldt and A. R. Wallace are among the renowned naturalists who pioneered the study of Amazonian biodiversity, hundreds of years ago. Despite a long history of exploration, a large number of new amphibians continue to be discovered. The key to these discoveries lies in the use of genetic data for species detection. A monograph, published in the open access journal ZooKeys (Caminer and Ron 2014), found 11 candidate species among populations of what were previously considered two widespread species, Hypsiboas calcaratus and Hypsiboas fasciatus. Based on genetic samples from populations across six countries, the team lead by Marcel Caminer (Museum of Zoology at Catholic University of Ecuador), found unequivocal evidence of the existence of a large number of cryptic species. The genetic results were corroborated with detailed analyses of male calls and body shape and color. The study formally describes four of the new species identified. (Santiago Ron)
- January 20, 2014:
Herpetologist Robert Inger wrote "A frog is a frog is a frog." Frogs have a body plan that has not changed for 200 MY, suggesting an evolutionary constraint. But is this true? Body plans may be related to life-style such as arboreal or fossorial habits, or alternatively, constrained by evolutionary history. Vidal-García et al. (2013) analyzed the Myobatrachidae of the Australo-Papuan region, a radiation with a remarkable diversity of sizes and shapes. Examining the relationship between environmental variables (such as temperature, humidity, topographic slope) and body measurements, they found no clear relationship between environmental niche and body size or shape when taking phylogeny into account. In seeming contrast, they also found that long-legged frogs inhabit wet areas and short-legged species inhabit dry areas. Intermediate species are found in a diversity of habitats. What this means is that both phylogeny and environment are important for "frogness"; evolutionary history has constrained the radiation of certain clades into specialized habitats.
- January 13, 2014:
Siphonops annulatus is a direct-developing caecilian, abundant in Brazil and northern South America, known for an unusual form of maternal care. The mother coils around her clutch of eggs, and the young hatch late in their development. They immediately commence eating the outer layers of the mothers skin, which has become thickened with lipids and turns pale gray rather than the more normal blue-black. Gomes et al. (Zoology, 2013) have carefully documented the metabolic, endocrine, and relevant morphological changes that take place during the reproductive cycle of these dermatophagic (skin eating) caecilians. Their study contributes extensive data and analysis that aid in understanding the cycle and the skin response, and constitutes a baseline for endocrine studies of reproduction in caecilians generally. (MHW)
- January 6, 2014:
2014 is the Year of the Salamander so it is fitting to highlight a report of the first antimicrobial peptides isolated from the skin of a salamander, Cynops fudingensis, a red-bellied newt from China (Meng et al 2013). Specifically this is only the second amphibian species found to have β-defensin antimicrobial peptides, besides the frog Rana chensinensis, although defensin peptides are known from other vertebrates. Likely this is part of the salamanders immune system, however, its exact role is still speculation and worthy of further investigation. (MK)
- December 30, 2013:
AmphibiaWeb ends 2013 with good news! Once again Mendis Wickramasinghe and his team (2013) have rediscovered a frog species from Sri Lanka that had been declared extinct. Pseudophilautus hypomelas, described by Guenther in 1876 based on 14 specimens and not observed for 137 years, was found between 750 - 1400 m elevation in the Peak Wilderness Sanctuary in the Central Hills. This is the third species thought to be extinct that was rediscovered by this team. About 40 individuals were observed, and three collected as vouchers, in habitat that is under high levels of threat. A change of status from Extinct to Critically Endangered is suggested.
- December 23, 2013:
AmphibiaWeb wishes all our users and contributors a safe and happy holiday season! 2013 was a productive year for amphibians and AmphibiaWeb with 146 new species, 944 new photos, 30 new recordings, 62 new species accounts and more to AmphibiaWeb; we are especially grateful to contributors who make AmphibiaWeb possible. As an educational, non-profit organization reliant on donations and grants, we ask those of you making year-end gifts to consider including AmphibiaWeb; funds go directly to support AmphibiaWebs mission in amphibian conservation and scientific synthesis.
Enjoy artwork from the cancelled film, Newt, courtesy of Pixar, as a nod to the upcoming Year of the Salamander, 2014!
- December 16, 2013:
Robert C. Stebbins, who died recently at the age of 98.5 years, published several classic studies on amphibians, notably his monograph establishing the ring-species Ensatina and his study of their natural history. His early book on Amphibians of Western North America was a ground-breaking study, which led to his well-known field guides. His last book, on California amphibians, was published in 2012. All were illustrated by him. At the time of his death, colleagues and friends wrote to express their condolences. Their memories and an interview with him published a few years ago in Copeia are part of a memorial document that stands as a tribute to a remarkable scholar, educator, communicator and artist. Also read a preview of his 2012 memoir, Connecting with Nature, the out-of-print California Amphibians and Reptiles (1972) and the out-of-print The Lives of Desert Animals in Joshua Tree National Monument with Alden Miller (1964).
- December 9, 2013:
Stynoski et al. (2013) reports from observations and experiments conducted on the Strawberry Poison frog, Oophaga pumilio from the Poison Dart frog family, that toxic alkaloids are present in all life stages, not only as adults. However, if tadpoles are fed eggs of a non-toxic and unrelated frog, they have fewer alkaloids than when fed conspecific nutritive eggs. This suggests that provisioning by females contributes significantly to adult toxicity. Further, the ability of Strawberry Poison frog females to arm tadpoles with alkaloids may offer an explanation for selective advantage of nutritive eggs.(DW)
- December 2, 2013:
The frog family Rhinodermatidae has just two species in the genus Rhinoderma, called Darwins frogs, which are restricted to Chile and Argentina. Discovered by Charles Darwin during his voyage on the HMS Beagle, Darwins frogs are known for their unique reproduction of their tadpoles developing in the vocal sac of their male parent. Sadly, these frogs are in trouble: the Chilean endemic R. rufum has not been seen since 1980, and R. darwinii has experienced population declines in Chile and Argentina. Soto-Azat and collaborators (2013) investigated whether chytridiomycosis was involved in the disappearance of R. rufum and decline of R. darwinii in Chile, by testing museum specimens and live frogs for the presence of the fungal pathogen with quantitative PCR. Among museum specimens, all infected Rhinoderma had been collected from 1970 to 1978, suggesting an introduction and onset of the epizootic during that decade. Among live frog populations, the prevalence of infection in sympatric amphibians was much higher in sites where Darwins frogs had become extinct or severely declined than in sites where they had not declined. These findings implicate chytridiomycosis for yet another group of amphibians that suffered enigmatic declines over the past four decades. (ACatenazzi)
- Novermber 25, 2013:
One of the defining characteristics of amphibians is their unusual skin. Many people know about the poison-dart frog species which produce neurotoxins on their skin to protect themselves from predators. Their bright colors advertise the fact that they are dangerous. In fact thousands of species likely produce compounds that serve to protect and also to communicate with conspecifics. Little is known however about the microorganisms that live on amphibian skin. A new study (Kueneman et al. 2013) uses high throughput DNA sequencing to describe the microbiota of 5 species of California amphibians. The study reports that microbial assemblages are strongly influenced by the species of host amphibian and they differed systematically from the microbial assemblages in their environments. This suggests that the skin microbiome and the amphibian hosts have close symbiotic relationships and may play an important role in amphibian host phenotype including disease resistance. (VV)
- November 18, 2013:
Conservation studies typically focus on creating management recommendations in isolation from public attitudes towards species. A recent study by Reimer et al. (2013) used social science techniques to investigate how knowledge of the eastern hellbender (Cryptobranchus alleganiensis alleganiensis) affected individual attitudes toward the species. They found that amongst survey respondents who were unfamiliar with the species, when given a little information about the rarity and threat level of hellbenders, participants were more likely to have more positive attitudes toward the species. Increased information did not affect the responses of individuals familiar with the species, who also tended to have the most positive attitudes. But those who were unfamiliar and not given more information had the most negative attitudes. Their work shows the importance of public outreach when making management decisions about rare and/or threatened species to ensure smooth adoption of conservation measures. (AChang)
- November 11, 2013:
Introduced Rhinella marina in Australia co-occur with many different species of native frogs. Lab experiments by Cabrera-Guzmán et al. (2013) show that tadpoles of three native tree frogs (Litoria) have negative effects on toad tadpoles by direct exploitative competition. These findings suggest that reintroduction of native frogs into areas where losses have occurred may be an effective method of controlling the introduced toads. (DW)
- November 4, 2013:
Is similarity in morphology, ecology, and performance (e.g. jumping, swimming, etc., abilities) across species assemblages caused by evolutionary convergence or by dispersal of evolutionarily conserved ecotypes? Moen et al 2013 analyse the ecology, morphology and performance of frog assemblages from Asia, Australia and South America to see whether biogeographic dispersal or convergent evolution best explained similarities across continents. They report three conclusions: 1) microhabitat similarities fostered convergence of morphology and performance no matter clade or continent; 2) in other species, similarities relied more on dispersal of evolutionarily conservative groups; 3) at least in one clade, Litoria, its arboreal specialist ancestor radiated into a range of microhabitat specialists, thus the exception to ecologically conservative dispersal.
- October 28,2013:
Competition with non-native species is often cited as a possible cause of decline in amphibians. Researchers in France (Cayuela et al 2013M) tested this hypothesis with the native threatened yellow-bellied toad, Bombina variegata, and invasive marsh frog, Pelophylax ridibundus while also taking into account three differences in their biology: breeding pond preference (pond-area dependent), palatability to predators (fish dependent), and adaptations to variations in hydroperiod (floodplain-width dependent). The researchers found that the two species were infrequently found in the same ponds and that pond area was the best factor explaining their cohabitation with species co-occurring in ponds of intermediate size. The results indicate that there is little competition between the two species and thus marsh frogs, in this case, are not contributing to the decline of yellow-bellied toads. (AChang)
- October 21, 2013:
Batrachochytrium dendrobatidis (Bd) occurs in caecilians (Gower et al 2013). More than 200 specimens specimens from Africa and South America were field-swabbed. Of the 4 families, 7 genera, and 8 species of African caecilians sampled, Bd was present in nearly all (except Schistometopum); it was not present in any of the 4 families, 5 genera, and 10 species examined from South America. Mortality, apparently from Bd, occurred in captive Geotrypetes seraphini, both field-caught and from the pet trade. It is important to sample caecilians because Bd heretofore had been reported only from batrachian amphibians (frogs and salamanders), and better understanding of the natural history of Bd and the conservation threat it poses will be gained from broader taxonomic and geographic sampling. (MHWake)
- October 14, 2013:
Well-preserved and articulated frog fossils are uncommon, but fossils preserving the external anatomy of frogs are exceedingly rare. A recent study by Laloy and colleagues investigates the internal anatomy of a "frog mummy" found in the 19th century in France. By studying the skeleton of this unique specimen, we now have a "face" for an enigmatic genus of frogs (Thaumastosaurus) known from the early Cenozoic of western Europe. The detailed study of the skeleton, which included parts previously unknown for this extinct genus, reveals affinities to ranoid frogs in Africa rather than to ceratophryids from South America as thought for the past several decades. (DBlackburn)
- October 7, 2013:
The Neotropical harlequin toads, Atelopus, have been extirpated from much of their original range, and the fungal disease chytridiomycosis is a proposed culprit for many of the extinctions. Most of the remaining harlequin toads are in lowland areas where elevated temperatures are thought to limit the growth of the fungus. Sandra Flechas and collaborators have explored an alternative hypothesis that symbiotic skin bacteria protect the toads from chytridiomycosis. The researchers compared the anti-fungal properties of cutaneous bacteria among three species of Atelopus. They found that A. elegans was infected with chytrid fungus near sea level on a tropical island, despite high temperatures, as well as harbored bacteria with the strongest anti-fungal properties among the three species examined. Thus, the cutaneous bacteria may enhance fitness by preventing or delaying the symptomatic phase of chytridiomycosis, and are candidates for the development of probiotic treatments against chytridiomycosis. (ACatenazzi)
- September 23, 2013:
In Memoriam - Robert Stebbins, 1915 - 2013.
We are sad to announce the passing of Bob Stebbins, a friend, a colleague, Curator and Professor Emeritus at the Museum of Vertebrate Zoology, UC Berkeley. He was 98 years old and his impact on us professionally and personally go far beyond the field of herpetology.
- September 16,2013:
Partners in Amphibian and Reptile Conservation (PARC) has designated 2014 as the Year of the Salamander. They are holding a logo contest, the deadline for submissions is October 1, 2013 as well as a photo contest for a monthly photo with inclusion in a calendar. For more details see PARC news. Look forward to a year of salamanders!
- September 9, 2013:
Batrachochytrium dendrobatidis has been written here many times as the chytrid fungus that causes the deadly disease chytridiomycosis, a leading cause of amphibian declines. When the devastating population crash of fire salamanders, Salamandra salamandra, in the Netherlands failed to turn up Bd, An Martel and colleagues (2013) uncovered a new species of Batrachochytrium, newly named B. salamandrivorans. They report on its lethality as well as its apparent lower thermal preference and resistance in the midwife toad (Alytes obstetricans), indicating differences in these closely related chytrid fungi. (MK)
- September 2, 2013:
Glass frogs are not as delicate as their name may imply. A recent study documented aggressive territorial behavior among male Red-Spotted Glass Frogs, Nymphargus grandisonae (Hutter et al. 2013). Males were found to engage in repeated fights across multiple nights that sometimes resulted in injuries, hypothesized to be from this species characteristic humeral spines. The researchers also described six distinct types of calls in this species, including advertisement, courtship, territorial, encounter, distress, and release calls. View a video of dueling frogs from the authors.
- August 26, 2013:
A scientific team from the California Academy of Sciences, including AmphibiaWeb's David Blackburn, recently returned from an expedition to the Cameroon highlands in West Africa bearing an exciting cargo. Several species of live frogs including Cardioglossa gracilis, C. pulcra, Hyperolius ademetzi, H. riggenbachi and Xenopus longipes returned with the team to start as the core of an initiative to study their biology and thus better inform conservation efforts. 38% of Cameroon's amphibians are Critically Endangered, Endangered or Near Threatened, which makes this project all the more urgent. Little is known about the basic life history and reproductive biology of these species but that will hopefully change with this captive breeding study underway.
Read more about this at KQED Science. (MK)
- August 19, 2013:
Microcaecilia is a genus of what are thought to be common caecilians in South America; of the 12 known species of Microcaecilia, over half (7) were described only in the last three years. The most recently discovered species is Microcaecilia marvaleewakeae from the Guianan region of northern Brazil, where Maciel and Hoogmoed (2013) distinguishes this delicate, violet-colored caecilian from M. taylori, also from the region. This new species presents intriguing new questions about the biodiversity of these caecilians. It is notable for also being named in honor of Dr. Marvalee Wake and her work on caecilian morphology, reproductive biology, development, physiology, and evolution. (MK)
- August 12, 2013:
Free-living tadpoles are known to have many morphologies that are often attributed to adaptations to their environment. Recent work by Rowley et al. (2012) describes a novel morphology from an oophagous, tree-hole dwelling tadpole, Rhacophorus vampyrus. These tadpoles have two backwards-facing hook-like teeth, which gives the species part of its common name, Vampire Flying Frog. Given the angle, it's unclear how the teeth are used in feeding, but the authors speculate that the hooks are used to flip trophic eggs into its wide mouth when feeding. (AChang)
- August 5, 2013:
The amphibian chytrid fungus (Batrachochytrium dendrobatidis, or Bd) is widespread in South Africa (Tarrant et al. 2013 in PLOS ONE). All threatened South African amphibians occur within the limits of the predicted distribution of Bd, in the wetter eastern and southern parts of the country. Bd was present on 14.8% of individuals tested belonging to 17 threatened species, but no adverse effects are reported, thus supporting the view that Bd is endemic to the region. (DW)
- July 29, 2013:
A new, richly documented study (Foden et al. 2013) of susceptibility to climate change among birds, amphibians and corals finds from 24% (optimistic) to 44% (pessimistic) of amphibians are highly vulnerable. Among the most vulnerable taxa are hemiphractid frogs, especially Gastrotheca, and plethodontid salamanders. The Amazonia is the most vulnerable area for amphibians. The authors conclude that global policies to mitigate greenhouse gas emissions have the potential to substantially reduce vulnerabilities. (DW)
- July 22, 2013:
Over the past two decades there has been an abundance of reports on amphibian declines. However, the rate of population losses has not been documented. Using data from the U.S. Geological Survey's Amphibian Research and Monitoring Initiative (ARMI) between 2002 and 2011, Adams et al. (2013) calculated the probability of amphibian occupation of ponds and other habitat in the United States for 45 species. Monitored sites included protected land with conservation policies in place. They found that overall occupancy declined at an annual rate of 3.7%, and species considered Endangered, Vulnerable, or Near Threatened by the IUCN declined at a rate of 11.6%, while species considered Least Concern by the IUCN declined at a rate of 2.7%. Amphibian declines appear more critical than first expected as even Least Concern species and those in protected areas are in decline. (AChang)
- July 15, 2013:
How has Batrachochytrium dendrobatis (Bd), the fungal agent of chytridiomycosis, become so widespread? Vredenburg et al. (2013) bolsters a long held theory that the global distribution of Xenopus species for the pet-trade and biomedical uses may have facilitated the spread Bd. Testing 178 museum specimens, they found positive Bd results from 2.8% of Xenopus specimens collected in the 1930s in Africa, and 13% positive Bd rate from frogs collected from the wild in California. Given that established populations of Xenopus were documented in the wild in California as early as the 1970s, these finding support the hypothesis that Bd could have been introduced from Africa to North America via Xenopus laevis or Xenopus tropicalis. (AChang)
- July 8, 2013:
Reintroduction of amphibians to sites that have experience local extinctions is an increasingly popular conservation strategy. A new study (Zeisset & Beebee 2013) of introductions of Bufo bufo in Great Britain has shown that size of the introduced population (>1,000) and the presence of different genotypes, derived from a geographically and environmentally distant location, are the most important variables in successful transplantations. Local adaptation was shown to evolve relatively rapidly. (DW)
- July 1, 2013:
Some good news about Mexican frogs! Stream-breeding hylids have been difficult to find in the Mexican Oaxacan highlands. Recent fieldwork (Delia et al. 2013.) reports the rediscovery of six species missing from recent surveys including Plectrohyla thorectes which had not been seen in 28 years. Delia et al also found Exerdonta abdivita which represents a new location for this little known species. Despite the other rediscovered frogs, the authors note some species of concern that have yet to be seen. (DW)
- June 24, 2013:
Bad news for Darwins frogs (Rhinoderma)! A detailed analysis (Soto-Azat et al. 2013 ) of the distribution and status of the two species finds that no specimens of Rhinoderma rufum can be found and it is inferred that the species became extinct about 1982. Furthermore, R. darwinii has declined more than previously thought, although it still has a broad distribution, the authors suggest its status be changed from Vulnerable to Endangered. (DW)
- June 17, 2013:
The enigmatic Martinique Volcano Frog Allobates chalcopis, discovered in 1984 and not seen since the 1990s, has been rediscovered. This species is the only dendrobatid frog known from the Caribbean islands. Fouquet et al. (2013) revealed it still exists, and that its assignment to Allobates is correct and is genetically distinct from all other Allobates studied (the geographically closest species occur in northeastern South America). This frog occurs above 800 m elevation to the summit (1390 m) on Montagne Pelée. The authors argue that its conservation status should be changed from the current Vulnerable to Critically Endangered. The curious distribution is thought to be the result of overwater dispersal, possibly as long ago as the Late Miocene.
- June 10, 2013:
Not seen for nearly 60 years, the Hula Painted Frog from northern Galilee, Israel, was rediscovered in November 2011(Biton et al. 2013). The Hula frog continues to surprise as it is a true rediscovery of a living fossil! Not only does it still exist in a tiny remnant of the once vast Hula wetlands, it is a representative of a clade of large frogs otherwise known only from fossils occurring from the Oligocene to Pleistocene. The rediscovered species, properly re-named Latonia nigriventer, is also the largest living member of the family Alytidae. (DW)
- June 3, 2013:
Developmental biologists (Bloom et al 2013) have experimentally shown that only a handful of molecules control the gut morphology of tadpoles as diverse as the herbivorous African Clawed Frog (Xenopus laevis) and the carnivorous-and often cannabalistic- Budgetts Frog (Lepidobatrachus laevis). Exposing Xenopus laevis embryos to specific molecules altered their gut morphology to resemble those of the carnivorous tadpole species, despite 110 million years since their last common ancestor. The reverse procedure on Budgetts Frog likewise reverted their guts to the herbivorous condition. A remarkable new method for identifying the developmental mechanisms underlying ecologically and evolutionarily important variation. (MK)
- May 27, 2013:
It's not surprising that amphibians lose body mass when food and water are limited, but a recent study by Bendick and Gluesenkamp (2013) has found that at least one species, Eurycea tonkawae, exhibits reversible decrease in body length during drought conditions. Using six mark-recapture surveys, which took advantage of unique markings on each individual, the researchers took measurements of individuals before and after an unusually extreme drought season. They found that many of the individuals had significantly decreased body length by reducing their intervertebral space immediately after the drought, but later recovered length to greater than their initial sizes. The authors hypothesize that the smaller body length may be an adaptation to extended periods of limited food availability. (AChang)
- May 20, 2013:
Prevalence of the highly virulent amphibian chytrid fungus varies among species, elevations and seasons in places where the disease has become endemic. While we knew that the fungus in culture grows best between 17 and 25 Celsius, the effect of a frogs thermal behavior on chytrid infection was unclear. A new study (Rowley and Alford 2013) shows that in three species of stream-breeding rainforest frogs (Litoria lesueuri, L. serrata, L. nannotis), the frogs probability of chytrid infection declines as they spend more time above the fungus upper optimum temperature of 25 C. The frogs temperatures were tracked daily at four sites and over several seasons. These findings highlight the potential role of variation in thermal preferences among individuals in determining individual susceptibility and population-level differences in prevalence of infection. (ACatenazzi)
- May 13, 2013:
Genomic analysis reveals a complex evolutionary history. Genome sequences of 29 isolates of Batrachochytrium dendrobatidis (Bd) from around the world combined with data from 20 previously published isolates show that this potent amphibian pathogen has a complex history (Rosenblum et al. 2013). It is neither new, nor very old (the root is estimated at 104,000 years old and the Panzootic lineage at 26,499 years), and thus it fits neither the novel pathogen nor the endemic hypothesis, but parts of both. The pool of genetic variation is deeper than previously thought. The need to identify specific mechanisms associated with shifts in virulence remains acute. (DW)
- May 6, 2013:
Heres a problem: if you cant tell the sexes apart, how can you effectively conduct captive breeding as an option for endangered species? Geocrinia, which has 3 of its 7 species listed as Critically Endangered to Vulnerable, are typically monomorphic, meaning little physically distinguishes males and females, and now Hogan et al (2013) shows that a noninvasive test based on the frogs poop can sex them reliably. Using enzyme immunoassays, the ratio of testosterone metabolites and estrone conjugate metabolites indicates whether the origin of the sample was an adult male or female 100% of the time. The test was a little less reliable in juveniles. While hormonal assays are often used in behavioral ecology, this study demonstrates a utility in conservation as well. (MK)
- April 29, 2013:
Female amphibians are typically larger than males. The common explanation for this difference in sizesexual size dimorphism, or SSD is that selection for more offspring favors larger female size ("fecundity advantage"). Some evidence indicates that sexual selection may favor the opposite pattern, males > females, when males fight each other for females or territories ("male combat"). Using phylogenetic comparative methods and published data from 550 frog species on body size, male combat, clutch size, egg size, and parental case, Han and Fu (2013) found no relationship between male combat and SSD, contrary to previous studies, and only a weak association in some taxa between fecundity and SSD. Across all frogs, however, they unexpectedly found that parental care, especially male parental care, best explained reduced size dimorphism between males and females. Han and Fu's favored explanation is "relaxed fecundity," meaning that because parental care promotes offspring survival, the female is not under intense selection to maximize fecundity through increased body size. However, they admit that the other two hypotheses are not unequivocally rejected. (DC)
- April 22, 2013:
Rhacophorid frogs achieve remarkably high diversity on Borneo, where 41 species are known. A rich array of life histories are displayed by these frogs, and expanded molecular systematic studies reveal that direct development has evolved independently in Philautus in SE Asia (including many on Borneo) and in Pseudophilautus/Raorchestes in S Asia (Hertwig et al, 2013). Borneo is a significant amphibian diversity hotspot, a result of repeated dispersal and vicariance events. (DBW)
- April 15, 2013:
The geographic range of the California Red-legged Frog, Rana draytonii, has been rapidly contracting to the north and west in southern California in response to encroaching urbanization (Richmond et al. 2013). The problem is exacerbated by periodic devastating fires and floods, with increasing difficulties for repopulation. Extinction debt of this sort will likely lead to more range restriction in the absence of aggressive management activities. (DBW)
- April 8, 2013:
Wickramasinghe and colleagues (2013) recently described 8 new species of Pseudophilautus all from an elevational gradient within one of the last remaining protected high elevation cloud forests in Sri Lanka, the Sripada World Heritage Site (Peak Wilderness). This is also home to the rediscovered Pseudophilautus stellatus, thought to be extinct for 160 years until last year (see March 11, 2013 News Box). This genus of rhacophorid frogs, numbered at 85 species including 16 extinct species, is entirely centered in Sri Lanka with many species known from a single locality. The new species are no exception and all but one are considered Critically Endangered. (MK)
- April 1, 2013: Aquatic salamanders feed using a Gape and Suck mechanism, with the mouth and throat expanding and drawing the prey into the mouth. New studies of feeding in Andrias davidianus (Heiss et al. 2013) reveal that their feeding mechanism differs from that of fish in that the suction is the result of rapid opening of the very broad jaws, and that throat expansion does not involve much active involvement of the hyobranchial apparatus. (See video.) This key innovation is thought to have freed the hyobranchial apparatus for independent evolution, leading through a long series of intermediate conditions to the extreme specialization associated with tongue-feeding in terrestrial salamanders. (DW)
- March 31, 2013:
On March 31, 2013, raise a toast to celebrate the 98th birthday of Robert C. Stebbins, Professor and Curator Emeritus, University of California at Berkeley. Bob remains active in his retirement and is now writing his memoirs, having already two other books in his 90s. While Bob is best known for his field guides of Western North American herpetology, he was also a pioneer crusader for conservation in general and for amphibians and reptiles in particular. He has published many scientific papers, notably his monograph on the Ring-species complex Ensatina in 1949, and is an accomplished artist. AmphibiaWeb extends our best wishes to our friend and colleague.
- March 25, 2013:
Each winter tons of de-icing salts, sodium chloride (NaCl) and magnesium chloride (MgCl2), are used on highways. Hopkins et al. (2013) studied the effects experimentally on embryonic survival and development in rough-skinned newts, Taricha granulosa. At environmentally relevant levels, both salts have severe effects. However, they also found variation among newt families, showing that natural selection leading to local adaptation is at least a possibility. But it may be too long to wait for adaptation, and action is required to reduce the negative environmental effects of road salt.(DBW)
- March 18, 2013:
Johnson et al. (2013) highlights the intersection of evolutionary history, restoration ecology, and conservation biology by testing three Tiger Salamander types found in California - Ambystoma californiense, Ambystoma tigrinum mavortium, and their hybrids - in experimental pools with different drying schedules. Illustrating the adaptive differences in the three types of Ambystoma, they found that non-natives and hybrids did the best in pools that dried more slowly, like man-made ponds in the highly modified California landscape, while natives did the best in pools that dried out more quickly and which simulated more naturalistic ponds found in California. (AChang)
- March 11, 2013:
What may have been the worlds most elusive frog, Pseudophilautus stellatus from Sri Lanka, last reported in 1853, has been discovered living in high elevation cloud forest in the Peak Wilderness of Sripada World Heritage Site (Wickramasinghe et al. 2013). The species is unusually large and colorful, but occurs in nearly inaccessible terrain. This was the first shrub frog described from Sri Lanka and it has long been considered to be extinct. Although in protected habitat (only 4.7% of Sri Lankas original rainforest survives), the area is suffering from forest dieback phenomenon and the expansion of invasive species, so its continued survival is not assured. (DW)
- March 4, 2013:
Batrachochytrium dendrobatidis (Bd) has extirpated amphibian populations and entire species, particularly frogs, in Central and South America. Although the fungus is known from all continents, many continental regions have not been sampled. Using DNA-detection methods and skin analysis, Penner et al. (2013) sampled 61 frog and 1 caecilian species, from seven West African countries from a variety of altitudes, habitats, and life histories. They also used environmental niche modeling (ENM), to predict the occurrence of Bd in the regions sampled. Thus, failure to detect the fungus would reflect its genuine absence. Although ENM predicted suitable environmental conditions for the fungus, no samples harbored Bd. Their analysis showed that the westernmost occurrences of previously documented occurrences of Bd stop at the Dahomey Gap, a naturally non-forested area. Localities at which the fungus was predicted by ENM but not found, are all west of the gap, suggesting its a true barrier. They suggested the most likely human-mediated mode of entry would be along transportation routes. (DCC)
- February 25, 2013:
The effects of dams are well documented on fish species (e.g., salmon) but less is known about how dams affect amphibians. A recent paper in Conservation Biology shows that dams negatively affect river-breeding frogs in California, such as Rana boylii. Kupferberg et al uses 20 years of frog breeding data from regulated (dammed) and unregulated (un-dammed) rivers to show that by altering the flow regime, dams are causing higher egg and tadpole mortality. Dammed rivers that add artificial peak flows in summer months, for example for boating recreation, are the worst for native amphibians. (VV)
- February 18, 2013:
In tests of the effects of fungicides, herbicides and insecticides on Rana temporaria in Germany, Brühl et al. (2013) found that applications at rates recommended on product labels led to mortalities as high as 100% (fungicides Headline and Captan Omya). All pesticides led to mortalities ranging from 20% to 100% after 7 days exposure at the label rate. Three products showed mortalities of 40% after 7 days exposure at levels one-tenth the label recommendation. These pesticides are widely applied, and it is evident that current regulations are not protecting amphibians living near application sites. (DW)
- February 11, 2013:
Parallel races to characterize amphibian biodiversity and at the same time to conserve it are mutually interdependent, argue Crawford et al. (2012). Using DNA barcoding methods, the authors sampled frogs in the ex situ collection of the El Valle Amphibian Conservation Center in Panama. Much genetic diversity was found. Three of ten species sampled displayed substantial genetic diversity. Whether species are described or not (e.g., Hemiphractus fasciatus could be divided into two or even three species), the authors argue that genetic data are central to establishment of successful captive assurance colonies. (DW)
- February 4, 2013:
A new quantitative, geographical analysis of Ecuadorian amphibians finds that chytridiomycosis and climate change are more likely causes for declines and extinctions than habitat loss (Menéndez-Guerrero & Graham 2013). Climatic niche properties of species appear to serve well as surrogates of the extinction risk of species (from IUCN Red List) and may prove useful as predictors of the vulnerability of species to climate change. Climate change, chytridiomycosis, and their synergistic interactions are likely having the greatest current impact on Ecuadorian frogs. (DW)
- January 28, 2013:
Amphibians play a prominent role in a recent re-evaluation of Wallaces famous work (1876) on zoogeographic regions of the world (Holt et al DOI:10.1126/science.1228282). It underscores the striking perceptiveness of Wallace. The authors compiled distributional and phylogenetic information for more than 21,000 species of amphibians (6110 of the 7089 currently recognized), birds and mammals. They identify 20 zoogeographical regions, which in turn they group into 11 larger realms, improving on Wallaces original interpretations. For amphibians, 19 zoogeographic regions are identified. Spatial turnover is greater in more southern parts of the world, where regions show the highest degrees of phylogenetic differentiation. Not surprisingly, Australia, Madagascar, and Atlantic regions of South America are species-rich areas that show the highest levels of phylogenetic uniqueness. (DW)
- January 21, 2013:
First described in 1998, the Tanzanian Kihansi Spray toad, Nectophrynoides asperginis, was already considered critically endangered. A dam built in 2000 reduced the spray from the waterfall which was critical for the toad and its habitat. A sprinkler system was later built to recreate pre-dam conditions. However in 2003, the populations plummeted due to a combination of chytridiomycosis, a sediment flushing dam operation, and a sprinkler failure during the dry season. The last record of this toad was in 2004, 6 years after its description. Fortunately, the Wildlife Conservation Society collected 499 individuals in 2000 and had them bred in several US zoos. Only 70 were left in 2004, until their requirements were understood, and as soon as 2012, the first attempt to reintroduce the toad was successful. Since then, 2500 US bred Kihansi Spray toads have been reintroduced to their native habitat. (JW)
- January 13, 2013:
Alternative hosts of the fungus Batrachochytrium dendrobatidis (Bd) that is responsible for many local and regional declines of diverse species of amphibians have been difficult to locate. Now a team of researchers (McMahon et al. 2013) has shown that crayfish (two species of Procambarus) in southeastern Louisiana harbor Bd zoosporangia within their gastrointestinal tracts. In experiments, crayfish, but not mosquitofish, were infected and held infection for more than 12 weeks. Bd was successfully transmitted to uninfected tadpoles of Rana sphenocephala. Exposure to water that had held Bd led to trauma and death in crayfish, suggesting that the fungus releases some chemical that has negative effects. These discoveries open new avenues of research on Bd and the possibility of its control in natural settings.(DW)
- January 7, 2013:
What causes amphibian mass die-offs? Although such events were often dismissed as circumscribed events in the past, they are now of great concern for conservation. Determining the cause of die-offs can be challenging and requires the integration of monitoring and analytical techniques. Rosa et al. provide an example of this approach by presenting evidence that an outbreak of chytridiomycosis caused a mass die-offs and population declines of the midwife toad in the mountains of Portugal. In the die-off triggering the study, hundreds of post-metamorphic toads were found dead in a national park in August 2009. Prevalence of chytrid infection in tadpoles in the region currently ranges between 15% and 100%, with some tadpoles being highly infected. Historically, the species occupied a much broader range in these mountains, suggesting that chytrid outbreaks may be linked to population declines, especially at elevations above 1200 m. (Alessandro Catenazzi)
- December 24, 2012:
AmphibiaWeb wishes you a safe and happy holiday season! We are grateful to the many citizen scientists, researchers and students who have contributed photos, recordings, species accounts and more to AmphibiaWeb. As an educational, non-profit organization reliant on donations and grants, we ask those of you making year-end gifts to consider including AmphibiaWeb; all funds go directly to support AmphibiaWeb and our mission in amphibian conservation and scientific synthesis. We look forward to 2013; we plan to roll out more new features to better serve the global community.
- December 17, 2012:
Most species formation in amphibians involves vicariant events and allopatry (Vences and Wake 2006). The discovery that very close relatives with small ranges occur together suggests that adaptive (e.g., ecological or behavioral) factors acting parapatrically or even sympatrically may have led to species formation. Vences et al. (2012 in Amphibia-Reptilia, 33: 503-520.) discovered that a new species of Malagasy treefrog, Boophis narinsi, is the closest known relative of B. majori, with which it is syntopic, or sharing the same habitat and range. The species differ slightly in molecular traits and have different male calls. The authors think the species arose in close proximity and suggest that other tropical species complexes should be investigated closely. (DBW)
- December 10, 2012:
The worlds 100 most endangered species includes several amphibians; this week, AmphibiaWeb highlights the Dusky Gopher frog, Rana sevosa, (also Lithobates sevosus) which had a historic range in the longleaf pine forest uplands and wetlands throughout the southeastern US. Its known range is now severely limited to only one county of Mississippi of 60-100 individuals. Habitat destruction and chytrid disease threaten this species. Although listed as Endangered by state and federal agencies, and Critically Endangered by the IUCN, more surveys are needed to monitor the sole known site and potentially discover more populations for protection before this species goes extinct. (MK)
- December 3, 2012:
What are the implications of amphibian loss for ecosystem dynamics? Whiles et al. (2012) undertook such studies in the Rio Maria in the eastern part of the Cordillera Central of Panama before (2006) and after (2008) a predictable (Lips et al. 2006) massive die-off and local extinction event caused by arrival of the infectious chytrid fungus (Batrachochytrium dendrobatidis). Typically such streams have harbored on the order of 18 co-occurring species of frogs and densities exceeding 50 individual tadpoles per square meter. Losses led to a 98% reduction of tadpole biomass and a corresponding increase of more than twice previously recorded levels of algae and fine detritus biomass, as well as a 50% reduction in nitrogen uptake rate. Grazing invertebrates did not compensate for the loss of tadpoles, as had been predicted. Loss of amphibians thus has ecosystem-wide consequences. (DBW)
- November 26, 2012:
Fossils contribute to the Tree of Life because they provide times for the splitting of branches in the tree. Based on fossils and DNA data, we can date the earliest split in the salamander tree at about 183 Ma (million years ago), in the Early Jurassic. This split resulted in two major branches of living salamanders: Cryptobranchoidea Hellbenders (Cryptobranchus) and Giant Salamanders (Andrias) and Salamandroidea, all other salamanders. Until recently, all known Jurassic fossil salamanders (146-200 Ma) have been on the cryptobranchoid lineage, not the salamandroid lineage. Gao and Shubin (2012) reports the first Jurassic salamandroid fossil, and named the Chinese fossil Beiyanerpeton, from deposits that are roughly 157 Ma. These animals were about 100 mm and were neotenic, as evidenced by impressions of the external gills. Their analysis shows that Beiyanerpeton lies deep on the stem leading to living Salamandroidea, thus filling the gap, as predicted, between the previously oldest salamandroid, Valdotriton, at 115 Ma, and the first branching event within living salamanders. (DC)
- November, 19, 2012:
IUCN's list of the 100 most endangered species includes several amphibians; this week, AmphibiaWeb highlights the sole salamander, Neurergus kaiseri. This strikingly colored newt is only found in three streams in the semi-arid Zagros Mts. of Iran, where not only local damming and introduced predatory fish, but also illegal poaching threaten these fragile populations. Despite CITES Appendix 1 listing, it is estimated there is less than 1,000 mature adults left in the wild. IUCN recommends steps to monitor and enforce laws against illegal trade, ensure habitat restoration and further legal protection.
Over the next few weeks we will highlight other highly threatened amphibians from the report. (MK)
- November 12, 2012:
Color polymorphism in amphibians is widespread but has been relatively little studied. Bell and Zamudio (2012) have examined sexual dichromatism in anurans, in which they recognize two kinds. Dynamic dichromatism occurs when males undergo a temporary color change during the breeding system. This ranges from subtle to dramatic, and is fairly widespread phylogenetically. Ontogenetic dichromatism involves changes in one sex, but not the other, related to ontogeny; only a few studies have documented the biological bases of such change. This study is the first to attempt a comprehensive, phylogenetically informed, analysis of these phenomena. Read more in AmphibiaWeb. (DBW)
- November 5, 2012:
The pipid frogs, comprised of four genera (Pipa, Hymenochirus, Silurana and Xenopus), represent one of the oldest living lineages of all amphibians and yet the history of their diversification (i.e. their evolutionary relationships) has been contested for many years. These frogs have a relatively rich fossil record and includes living members currently span across widely separated continents (Africa, North America and South America) with well-known geologic separation dates (the continents once were connected). Yet there is considerable disagreement about pipid frog evolutionary relationships. A new paper by Bewick et al (2012) used high throughput sequencing and public databases to generate a large phylogenomic dataset to estimate evolutionary relationships. They found strong evidence showing West African Hymenochirus is more closely related to the South American Pipa than to the other African pipid species, Xenopus and Silurana.
- October 29, 2012:
The frog Nasikabatrachus sahyadrensis represents an ancient lineage endemic to the Western Ghats of India. Described almost ten years ago, much remains unknown of the natural history of this unusual species. In a pair of Zootaxa papers, Zachariah et al. (2012) and Raj et al. (2012) described in detail the reproductive biology and tadpole of Nasikabatrachus. Females find males calling from individual burrows along streams, and mating and egg-laying occurs mostly in small pools alongside torrential streams. Large numbers of eggs are laid and fertilized (>1000), and within a week tadpoles are fully formed. Unusual for a fossorial frog, the tadpoles are specialized for living in fast moving streams, having a stream-lined appearance and a large suctorial mouth used for rasping rock surfaces, including outside of the water and well into metamorphosis. Zachariah et al. (2012) add new distributional records and stress that recent irregularities in monsoon rainfall may put this species at risk because of its larval biology. (DB)
- October 22,2012:
Field surveys revealing the presence of either declines or disease are still relatively uncommon for African countries. Gower et al. (2012) present the first surveys for the chytrid fungus Bd at montane sites in Ethiopia, including for the Bale Mountains which are in important region of endemism for amphibians. Based on surveys in 2008 and 2009, more than 40% of examined individuals were infected with Bd, though largely with low intensity infections. Prevalence tended to be higher in species with aquatic larvae, and in two such species (Phrynobatrachus minutus and Leptopelis gramineus), prevalence was greater than 60%. While no mortality events were observed related to Bd, declines in Ethiopian amphibians are apparent and the role of disease in these declines remains uncertain. (DB)
- October 15, 2012:
Congratulations to John Gurdon of Cambridge University, UK, who has been awarded the Nobel Prize in Physiology or Medicine for his research, first published in 1962, which paved the way for modern stem cell research. He tested the hypothesis that cells retain the potential to return to pluripotency, the capability to differentiate into any cell type, by replacing the cell nucleus of a frog's egg cell with a nucleus from a mature, specialized cell derived from the intestine of a tadpole. The egg developed into a fully functional, cloned tadpole. Throughout his long and prolific career the experimental organism of choice in Gurdons lab has been the African Clawed Frog, Xenopus laevis. Gurdon shares the prize with Shinya Yamanaka, Kyoto University and University of California, San Francisco, who does not work with frogs.
- October 8. 2012:
Tiny animals have been postulated to differ from large ones in traits related to overall evolutionary diversification. Wollenberg et al. (2011) postulated a "microendemic phenotype" or MEP for frogs, in which the combination of small body size and small range size should result in increased rates of speciation in clades with these characteristics. The Malagasy-endemic frog clade Mantellidae has mostly small species, the smallest of which is 13 mm (maximum male body size). Using an extensive phylogeny and correlation analysis of 257 species (including candidate species) of the Malagasy-endemic Mantellidae, they supported the existence of a MEP. They also hypothesized that clades with the microendemic phenotype (small body size and small range) would have greater species richness, but were unable to support this hypothesis. However, they found that small size alone tends to predict species richness. They conclude that, contrary to expectations of increased diversification, the combination of small body size and small range size results in a slow-down in rate of diversification, as would be expected in the later stages of adaptive radiation. (DC)
- October 1, 2012:
September 26, 1912, was the birthday of Professor-Emeritus Hobart Muir Smith of the University of Colorado, Boulder, who reached 100 years of age last week. We congratulate Hobart and celebrate his spectacular scientific productivity, which includes about 1500 papers and books. While best known for work with reptiles, Hobart made significant discoveries on amphibians during his long field trip to Mexico starting in 1932. Hobart devoted his life to the herpetology of Mexico. He named many species of amphibians, and two salamanders (including Thorius smithi above) and two frogs named for him are currently recognized. AmphibiaWeb salutes Hobart Smith and sends him our congratulations and very best wishes. See the tribute video that researchers at UNAM (National Autonomous University of Mexico) created in Spanish.
- September 24, 2012:
The cause for some amphibian declines has been linked to global climate change, most recently by Lowe (2012) in the stream breeding amphibian Gyrinophilus porphyriticus. Between 1999 and 2010, Lowe found a significant decline in abundance of G. porphyriticus adults, with no trends found in larval abundance. Annual precipitation is predicted to increase in Northeast USA due to climate change, and adult G. porphyriticus are negatively correlated with higher annual precipitation. Survival during metamorphosis also declined dramatically during this time. These results suggest that increasing precipitation is causing a decline in adults and metamorphosing larvae, which could lead to local extirpation of this species. Its possible that metamorphosing individuals are dying during spring and fall floods, which have increased in volume and frequency in the past 12 years. There is a critical need to collect population data on more stream-breeding amphibian species. (CS)
- September 17, 2012:
For many little known amphibians, museum collections are the only form of information on them. These historic data can be put to use with modern observational records as Akmentins et al (2011) show for the three Gastrotheca species of conservation concern in Argentina. They used probabilistic modeling with museum records and recent rediscovery data on Gastrotheca gracilis to test the assumption that G. gracilis, G. christinani and G. chrysosticta, all endemic to the southern Andean Yungas montane forest, still survive. Their predictions are that while likely still extant they are in severe decline and should be moved to the Critically Endangered listing. (MK)
- September 10, 2012:
Understanding the disease dynamics of occurrence and outbreak in space and time is an important goal of studying the deadly chytridiomycosis and its causative agent, the fungus Batrachochytrium dendrobatidis (Bd). A recent study (Velo-Antón et al 2012) used population genetics techniques on Bd from western North America through Central America and found that Bd conforms to the Spreading Pathogen Hypothesis where pandemics occur after exposure to naive populations. It is clear Bd is a novel pathogen that spread southward from North to Central America and that future conservation efforts should include both research on the genetic variation of its pathogenicity as well as public education and trade restrictions to curb its spread. (MK)
- September 3, 2012:
Exploiting competitive mechanisms to control an invasive species. Cane toads, Rhinella marina, are invasive in Australia, where they are rapidly advancing into new terrain. Their tadpoles consume eggs of co-occurring species and impact native species populations greatly. Crossland et al. (2012) show that toxins (mainly bufadienolides) extracted from cane toad eggs serve as a cannibal attractant when used to bait funnel traps, which caught tens of thousands of cane toad tadpoles. Because native frogs do not produce bufadienolides and are not attracted to them, baited traps can be used in a strategy to control cane toad populations. (DW)
- August 27, 2012:
No other amphibian has the panoply of bright colors as those found in populations of Oophaga pumilio, the Strawberry Dart frog: red, blue, green, yellow, arranged in a diversity of spotted, blotched, and solid patterns. Such variation is thought to be due to sexual selection, i.e., females choose certain mates over others. Thus the inheritance of certain traits is not random. In previous lab studies of one population with only red and yellow individuals, females preferred males of similar coloration. But what do animals do in nature? Richards-Zawacki et al. (2012) used a more direct method of identifying successful matingspedigree analysis. Using genetic analysis of parentage, they determined that in nature yellow females were less choosy; they mated with red or yellow males more or less equally. However, red females were very choosy and mated only with their own morph. Many reasons (visual sensitivity, the effort that yellow females may use to find mates, etc.) might explain the differences in lab and field behavior. But importantly, the study emphasizes the need for multiple approaches to the same biological question. (DCC)
- August 20, 2012:
A new study based on resurveys in the Great Smoky Mountains National Park (southern Appalachian Mountains) finds no clear answers for the almost 50- year widespread decline in plethodontid salamander populations. In general, Caruso and Lips (2012) found declines not associated with localities but with certain Plethodon species, such as P. glutinosus and P. teyahalee. Normally associated with such widespread amphibian declines, chytridiomycosis was ruled out after they found only one out of 665 salamanders with Bd. Instead they found mixed correlation with climate change and morphological differences among salamander species which declined and those which did not or even increased in populations. Amphibian declines in the eastern US are poorly studied and thus remain enigmatic. This study is important to understanding declines in the heart of diversification for the Plethodontidae family. (MK)
- August 13, 2012:
One of the new species of treefrogs has been named for an equally colorful personage, His Royal Highness Charles, Prince of Wales. Dr. Luis Coloma, Director of the Centro Jambatu in Ecuador, and his colleagues have named a colorful frog from northern Ecuador ( Coloma et al. 2012). The patronym Hyloscirtus princecharlesi honors the Prince for his efforts in preventing deforestation in tropical habitats crucial to the survival of millions of species. The species is one of a hidden lineage of frogs dwelling in the high Andes, unknown until museum expeditions first entered these forests about 40 years ago. The Prince Charles Stream Treefrog will likely stand as a beacon for the worldwide amphibian conservation effort. The announcement of the new species took place at a meeting between Dr. Coloma and Prince Charles at Highgrove House, an exchange facilitated by Amphibian Ark (Watch the video here). (DCC)
- August 6, 2012:
Another milestone: The 25,000th photograph posted on AmphibiaWeb is of a recently described species, Bolitoglossa aureogularis, taken by Roney Samaniego and Eduardo Boza-Oviedo. The photograph is of the adult female holotype, found in 2007 near the crest of the Trans-Talamancan trail in a remote part of the Cordillera de Talamanca, Costa Rica (Boza-Oviedo et al., Zootaxa 2012). The species represents a previously unrecognized clade that also includes the recently named Bolitoglossa robinsoni. Thanks to Photographer Eduardo Boza-Oviedo, who has contributed 122 photographs of 52 species of amphibians to AmphibiaWeb.
- July 30, 2012:
The total number of amphibian species reached 7,000 today. The 7000th known amphibian is a new glassfrog from Peru, Centrolene sabini (Catenazzi et al 2012), which was discovered at high elevations in Manu National Park, Peru. Glassfrogs have increased from 65 in 1985 to 152 known today, illustrating the paradoxical phenomenon of amphibian discovery during a time of great concern for amphibians. In June 2012, IUCN reported 41% of amphibian species at risk of extinction. Yet, the number of known amphibian species has increased dramatically, from 4,013 in 1985 to 7,000. Enjoy AmphibiaWeb's new song in celebration of the 7000th species!
- July 23, 2012:
Many factors affect the success of non-native species introductions. A new study by Rago, While and Uller (2012) found that similarity between native climate and introduced locality climate have more to do with amphibian introduction success than species traits, such as phylogeny, ecology and life history. This contrasts with other recent work showing that species traits were major factors for introduction of non-native species. Using life history data and geographic range size from multiple sources, including AmphibiaWeb, IUCN, primary literature and field guides, Rago et al. analyzed establishment success. Climatic similarity between the native range and introduced locality was the strongest predictor for establishment success. Pathways of introduction were also a strong predictor, with intentional introductions being more likely to succeed than non-intentional ones. This study shows that human-mediated introductions and movement of species in similar climatic envelopes are major factors affecting success of amphibian introductions. (CS)
- July 16, 2012:
The New York Times reports that "Frog Juice" is being used secretly and illegally in the US horse racing industry as a pain suppressant, intended to enhance performance. It also makes the horses feel more excitable and euphoric. The active agent is dermorphin, an opioid first isolated from the skin of Phyllomedusa sauvagii and probably present in related species. It is well known that amphibian skin is a veritable pharmacopoeia, producing diverse alkaloids, opioids and other secondary compounds. Dermorphin is reported to be 40 time more effective than morphine in reducing pain, but side effects are less well known in either equine or human subjects. (DW)
- July 9, 2012:
The timing of egg hatching and development is known to be highly adaptive and thus under selective pressure. Many studies have shown the effect of the timing of early stages has on fitness. Recently Hopkins et al. (2012) showed a high level of variability of these traits in the Rough-skinned newt, Taricha granulosa, which is independent from the habitat temperature. Moreover the values of the traits and the degree of differences between the study females lineages could not be explained by morphological traits of mothers nor the eggs. They suggest that the variation necessary for selection to work on is present at the earliest of life history in this amphibian, which seems especially important given the presence of seasonal predators such as dragonfly nymphs. (JW)
- July 2, 2012:
In June, the US Environmental Protection Agencys Scientific Advisory Committee met to conduct an ecological risk assessment of Atrazine, an herbicide that is associated with a wide variety of problems in amphibians. One of the most notorious studies on Atrazine, an estrogen disruptor, was linked with amphibian sex reversal when applied to amphibian larvae in ecologically relevant doses (Hayes 2002). Since that publication in 2002, there have been many calls for the ban of Atrazine use in the United States (it is already banned in the European Union). The EPA will be reviewing scientific research on the effects of Atrazine on aquatic communities, including amphibians, in anticipation of the upcoming registration review, set to take place in 2013. (Jamie Voyle)
- June 25, 2012:
A newly published study (Rosenblum et al. 2012) reveals that frogs as phylogenetically diverse as ranids and pipids display a shared genetic response to infection by the chytrid fungus Batrachochytrium dendrobatidis. The authors characterized transcriptomes of Rana muscosa and Rana sierrae in experiments that controlled for Bd infection, and compared the results with comparable studies of Xenopus tropicalis. The infected frogs show massive disruption of skin function and no robust immune response. (DW)
- June 17, 2012:
Just in time for Fathers Day in the US, UK, and elsewhere. Male parental care of eggs and young is unusual in frogs, but evidence continues to build that a number of members of the microhylid subfamily Astylosterninae, especially Cophixalus, Austerochaperina and Liophryne from Australia and New Guinea, have male parental care of terrestrial, direct developing eggs (Hoskin 2004). Males of some astylosternines even transport newly hatched froglets (Bickford 2002). AmphibiaWeb lists 266 astylosternines, and more are discovered every year. A recent discovery (Hoskin 2012) is Cophixalus hinchenbrookensis, known only from Hinchinbrook Island off the central coast of Queensland, in which males guard eggs. All of these frogs eat ants and it has been suggested that ants might be the major egg predators. (DW)
- June 11, 2012:
An association between rates of metabolism and molecular evolution is not a new idea. Yet, previous work on ectotherms has not supported an association between rates of molecular evolution and resting metabolic rate. Using data for poison frogs in the family Dendrobatidae, Santos (2012) combines diverse information for resting and active metabolic rates, phylogenetic relationships, body mass, fecundity, and rates of evolution for both mitochondrial and nuclear genes. His analysis suggests a strong positive relationship between rates of active metabolism and molecular evolution and hint at a possible mechanism for this relationship based on an increase of reactive oxygen species during active exercise. (DB)
- June 4, 2012:
Phrynobatrachus is a widespread African genus of 85 currently recognized ranoid frog species usually placed in their own family. Zimkus et al. (2012) conducted a phylogenetic analysis of a large number of species of Phrynobatrachus to determine correlates of miniaturization and terrestriality, both represented in numerous species. They hypothesized that miniaturization is correlated with terrestriality, but such proved not to be the case. Instead, while each has evolved multiple times, the two trends are decoupled. Thus neither trait constitutes a key innovation for an adaptive radiation associated with the diversification of this taxon. (DW)
- May 28, 2012:
More than half of the truly diminutive frogs (46 species defined as less than 15 mm snout-vent length(svl)) have been described since 2000. Annandale described Pseudophilautus semiruber, a diminutive upland frog, in 1913 but has not been seen for a century until recently. Its rediscovery from the Agra-Bopath forest at 1,750 m a.s.l. led to research by Meegaskumbura et al. (2012) showing that it and the distantly related P. tanu are the smallest members of Pseudophilautus. P. semiruber (12-13.4 mm snout to vent) is the sister taxon of the genetically distinct (4% different in 16s mtDNA sequences) P. simba, which is only slightly larger (12.6-15.6 mm svl). (DW)
- May 21, 2012:
Salerno et al. (2012) found that the ancient tepui summits in South America harbor young, rather than old, lineages of frogs. The tepuis (flat-top mountains) of South America, known as sky islands, were the inspiration for Sir Arthur Conan Doyles novel The Lost World. These are ancient remnants of the Precambrian Guianan Shield plateau and were formed 70-90 mya. Because of their age, it was thought that the fauna of the tepuis may also be very old, and the tepuis may have served as a refuge for these species. But Salerno (et al. 2012) found that treefrogs of the genus Tepuihyla climbed up the 1000-meter tepuis from the lowlands within the last 2-5 mya. Currently there is no empirical evidence for the Lost World hypothesis. (CS)
- May 15, 2012:
Carlos Vasquez Almazan, a Guatemalan amphibian expert, is one of this year's Whitley Fund for Nature Awards winners. Carlos created Guatemala's first network of protected areas for endangered amphibians, including critical habitat for Duellmanohyla soralia, endemic to the Sierra Caral and Sierra Merendon along the Honduras-Guatemala border. View the video on Carlos and Guatemala narrated by Sir David Attenborough.
- May 7, 2012:
The evolutionary effects of contemporary human construction are not well known. Steven P. Brady (2012) has shown that roads could be a novel agent of selection on Ambystoma maculatum in a human-altered landscape. While negative ecological effects are evident, he addresses potential responses of local populations to the harsher roadside conditions, which would lead to differential survival ability across the whole population. After a translocating experiment, he found that roadside living sub-populations were more able to survive than those which live away from the road. Moreover his study suggests a role for a genetic component in explaining the local adaptation, offering the hope that vertebrates may adapt to anthropogenic disturbance on contemporary timescales and across small spatial scales. (JW)
- April 30, 2012:
Chytridiomycosis, the amphibian disease caused by the fungal pathogen Bd, has decimated numerous amphibian species around the world. Investigation of Bd's deadly effects have so far primarily been done in captivity, with lab-cultured strains that may have lost some virulence. A new PLoS ONE paper by Voyles et al. (2012) is the first to examine blood chemistry of infected frogs in the wild during an outbreak of chytridiomycosis. The results show that electrolyte depletion (sodium and potassium) for heavily infected mountain yellow-legged frogs in the wild is even more extensive than studies done in captivity have suggested, and is accompanied by severe dehydration despite the frogs' aquatic environment. See also the NSF commentary.
- April 23, 2012:
Pipidae is a thoroughly researched frog group; Xenopus laevis is a model species and Silurana tropicalis is the single frog with a complete sequenced genome. Morphological analyses (including fossils) and earlier single gene analyses placed Pipa + Hymenochirus (Pipinae) in a clade with Xenopus + Silurana (Xenopodinae) as its sister clade. Because Pipa is South American and Hymenochirus is African, this phylogeny supports an African origin (African Root). Recent analyses of multiple genes place African genera in one clade in opposition to Pipa. Here, the origin of Pipidae cannot be more specific than Gondwana (Gondwanan Root). Bewick et al. 2011 using more than 100 genes analyzed with newer coalescent methods, found strongest support for the African Root Hypothesis, validating earlier phylogenies. Nonetheless, the authors identify general difficulties: large ancestral population sizes, ancient divergence times, and failure to identify duplicated genes (paralogs). (DCC)
- April 16, 2012:
The Indian subcontinent has long been recognized as an important center of biodiversity: India and Sri Lanka together have about 450 species of amphibians, and Indias Western Ghats is well known as a biodiversity hotspot (for an interesting account of Indian herpetologists at work in the Western Ghats see The Economist article). Now Kamei, Biju, and colleagues announce a new amphibian hotspot, the region where the recognized Himalayan and Indo-Burma hotspots make contact in northeastern India and northern Myanmar. The caecilian Herpele fulleri is shown to differ greatly from other herpelids, part of a previously undetected clade whose estimated divergence time from the herpelids is so ancient (possibly dating back to the beginning of the Cretaceous) that they designate the clade a new genus (Chikila) and family (Chikilidae). The other members of the new clade, all from the same general region, are at present unnamed. The authors suspect the region contains more hidden treasures. (DW & MW)
- April 9, 2012:
A new paper by Mokhatla and colleagues in Diversity and Distributions (2012) assessed anthropogenic threats on South African amphibian breeding areas. Terrestrial frogs were found more often than chance in breeding areas where anthropogenic land changes or high numbers of invasive plants occur. South-central South Africa is the only region where terrestrial frogs coincided with protected areas. The majority of regions where stream-breeding frogs occurred coincided with non-native plants and other anthropogenic effects. Breeding areas for both aquatic and terrestrial frogs in the southwestern Cape are most congruent with multiple anthropogenic threats. This study showed that the presence of non-native plants and anthropogenic-changed landscapes were important factors for management of breeding amphibians. Also, it highlighted areas where terrestrial breeding amphibians are not well represented in current conservation networks. (CS)
- April 2, 2012:
Invasive cane toads (Bufo marinus) in northwestern Australia have proven lethal to many native animals, which eat the toxic amphibian and are not adapted to its secretions. One such affected species is the omnivorous reptile Tiliqua scincoides, the bluetongue skink. A University of Sydney study, however, indicates that another invasive species, a plant native to Madagascar named mother-of-millions (genus Bryophyllum), may have helped some skink populations to develop resistance to the cane toads deadly toxins. The plant produces bufadienolide toxins similar to that of the cane toad (a result of convergent evolution), and both species are readily ingested by bluetongue skinks. Skinks from regions with introduced mother-of-million showed a higher resistance to bufadienolides than skinks from regions without the plant. Such preadaptation may have a positive impact on the persistence of bluetongue skinks, and other omnivorous Australian species in the face of the introduced cane toads. (John Cavagnaro)
- March 26, 2012:
How many species of Amphibians ARE there? With nearly 7,000 species described, one might think we are reaching a decisive answer. However, in many parts of the world, we are just coming to understand that herpetologists have overlooked cryptic species. Funk and colleagues report finding that species richness in Ecuador, which may have more species of amphibians per unit area than any other country on earth, has been severely underestimated. For example, two currently recognized species of Engystomops are shown to be from five to seven species, and two species of Hypsiboas are six to nine. Clearly, amphibian taxonomists still have much work ahead of them. (DW)
- March 19, 2012:
Although most new species of amphibians are being discovered in remote corners of the tropics, sometimes there are surprises: a new frog species has been reported in New York City (and surrounding counties). The as-yet-unnamed species of leopard frog resembles the southern leopard frog, Rana sphenocephala, but is clearly distinct from other local leopard frog species (R. sphenocephala, R. pipiens, R. palustris) both genetically and by its call, and has a restricted range. This new discovery highlights the importance of urban areas as well as pristine habitat in conservation of biodiversity. See both the paper by Newman et al. (2012) in Molecular Phylogenetics and Evolution, and the NYTimes and NSF perspectives. (KW)
- March 12, 2012:
AmphibiaWeb has a new taxonomy for families! You can read more about AmphibiaWeb Taxonomy 2.0 on our updated Taxonomy page. Our entire Browse species lists" has been updated to reflect the new family-level organization. AmphibiaWeb 2.0 is the result of in-depth studies of the current literature dealing with amphibian phylogenetics and taxonomy by a multi-institutional working group of taxonomic experts. From time to time, the taxonomy will be updated, as warranted, based on new analyses and publications. Comments are welcomed. We also solicit assistance in writing family and species accounts from specialists. We invite you to contact us!
- March 5,2012:
Despite amphibian species bearing the brunt of declines among most vertebrate groups (over 40% of known amphibians are in decline), they have garnered the least attention in terms of funding and capacity building to address this crisis. Gratwicke, Lovejoy, and Wildt (2012) quantify this disparity by comparing the funding per species listed under the US Endangered Species Act with NatureServes endangerment status, and find that listed US amphibians receive only a quarter of funding that other listed vertebrates do. They also reveal a larger disparity as 82% of amphibians that are listed at risk by NatureServe remain unlisted by the US, where many species are as threatened as their tropical brethren. (MK)
- February 27, 2012:
Invasive species often have surprising effects on their adopted environment, be it by direct interaction with other species or by altering the habitat. In a study by
Watling et al, the effects of an invasive plants phytochemicals on some species of amphibians have been reported. In one common species, the American toad, the presence of the introduced bush honeysuckles (Lonicera maackii) allelopathic compounds in the water decreases tadpole survival. In other amphibian species, the presence of the plant chemicals alters the behavior of the tadpoles, increasing the number of trips to the surface, which may be a way to counteract the toxic effects of the plant products. (JW)
- February 20, 2012:
The most diverse salamander clade in the Western US, Batrachoseps has recently grown in number to 22 recognized species, with the recent addition of B. altasierrae and B. bramei (Jockusch et al 2012). These two new species are found in the rugged southern Sierra Nevada of California, where now six species of Batrachoseps are known, highlighting this region as not only one of general high vertebrate endemism but also a center of Batrachoseps diversification. (MK)
- February 13, 2012:
California Protects Sierra and Southern California Mountain Yellow-legged frogs under State Endangered Species Act. Just a few decades ago, mountain yellow-legged frogs (comprised of two closely related species, the Sierra Nevada yellow-legged frog, and the southern mountain yellow-legged frog) were very abundant throughout their range in California and parts of Nevada, but declined rapidly. Widespread planting of nonnative trout from airplanes in high-elevation lakes has been a primary cause of the species decline. Introduced trout eat tadpoles and juvenile frogs and change the food web of the aquatic ecosystems on which the native frogs depend. Since 2000, agencies began restoring frog populations by removing nonnative trout from some high Sierra Nevada lakes; however, an emerging fungal pathogen ("chytrid fungus") has caused widespread mass die offs and more than half of the remaining populations identified in 1995 have disappeared. US Fish & Wildlife will rule on their status in October 2012. (VV)
- February 6, 2012:
One of the most enigmatic amphibians is the lungless caecilian Atretochoana eiselti. Edward Taylor described this species in 1968, based on an old specimen in the Vienna Museum, said to be from South America. Wilkinson and Nussbaum (1997) studied the type in great detail and described its lunglessness and associated features. Authors conjectured that the species must be aquatic; the only other known lungless caecilian is the tiny terrestrial Caecilita iwokramae. Now, Hoogmoed et al. (2011-PDF) report rediscovery of the species in Brazil, from Baía de Marajó, a tidal zone near Belém, but also from the geographically remote (2000 km) upper reaches of the Madeira River in Rondônia. The largest specimen is a meter long. How this species survives in such varied environments and how respiration takes place remain to be discovered. (DW)
- January 30, 2012:
Froglog, started 20 years ago by the Declining Amphibian Populations Task Force (DAPTF) and now published by the Amphibian Specialist Group, celebrates its 100th issue, 69 pages of amphibian research and conservation news from around the world. FrogLog continues with special regional focus issues, the latest on South American herpetology. Read the latest FrogLog directly on AmphibiaWeb! (KW)
- January 23, 2012:
Chytridiomycosis is thought of as exclusively an amphibian disease. However, it is clear that the causative fungus Batrachochytrium dendrobatidis (Bd) can persist in the environment and other non-amphibian species may serve as vectors. A recent paper by Kilburn et al. suggests that reptiles may spread the disease by having the fungus on their skin. The study in Panama surveyed 21 species of snakes and lizards and found Bd in varying intensities and was positively correlated with infection rates among co-occurring frog species. Whether reptiles simply act as vectors or reservoirs and are not likewise infected remains to be shown. (JW)
- January 20, 2012:
IN MEMORIAM: Joseph T. Collins, founder of the Center for North American Herpetology website, died on January 14, 2012, at the age of 72. He was one of the founders of the Ohio Herpetological Society, which became the Society for the Study of Amphibians and Reptiles, and was associated with the University of Kansas for many years. Roger Conant invited Collins to join him as a coauthor of the third edition of the Field Guide to Eastern Reptiles and Amphibians in the Peterson Field Guide series. Collins wrote extensively about the Kansas herpetofauna and long advocated for increased public awareness of amphibian and reptiles. (DW)
- January 16, 2012:
AmphibiaWeb added 149 species of amphibians to our database in 2011, the fewest since we began keeping track in 2004. However, 2010 was a big year, with 205 species added, and the passage of a year is a pretty arbitrary stopping point. The mean number since 2004 is 184. Most of the additions are new discoveries, but some are subdivisions of taxa stimulated by new discoveries. An example of this pattern is the new monograph in Zootaxa of the megophryid frog Leptolalax (Lalos) by Ohler et al. . New discoveries led to new analyses that showed formerly widespread species to be non-monophyletic, which in turn led to descriptions of truly new species and the resurrection of others from synonymy. (DW)
- January 1, 2012:
Happy New Years to all our users and contributors! As a community of amphibian enthusiasts, researchers and students, you have contributed almost 24,000 photos of 3,631 different amphibian species, and 2,810 species accounts out of the 6,909 total species of amphibians that we tally here at AmphibiaWeb. We sincerely thank you for all your contributions and for using AmphibiaWeb as your resource for information on amphibians, their biology. and their decline status. We look forward to an exciting 2012 with many new developments on tap, including updated taxonomy, new taxonomy tools, enhanced maps, and improving mobility with our iPhone app, which you can treat yourself to now (download from iTunes). Stay in touch with AmphibiaWeb as we move forward into 2012!
- December 19,2011:
The task to survey and monitor often cryptic species in the worlds aquatic systems such as ponds, streams, lakes, and wetlands is a daunting challenge. Biologists now have a powerful new tool which starts with just a shot-glass size sample of pond water. A Danish research team has shown that DNA traces in the water sample can be amplified and thus contain evidence of an entire aquatic fauna. The proof-of-concept study focused on species as diverse as the Eurasian otter, Weather loach (fish), Spadefoot toad (Pelobates fuscus), Great crested newt (Triturus cristatus), Large white-faced darter (dragonfly), and the tadpole shrimp. This may be the future of biodiversity monitoring, using DNA traces in the environment to discover and keep track of threatened wildlife. (MK)
- December 12, 2011:
In a November 2011 Letter to Nature, Hof, Araújo, Jetz and Wahbek report that multiple threats from pathogens, land-use and global climate change affect amphibian populations, and that together, these additive, multiple effects will likely cause accelerated declines and extinctions in the 21st century. Using current distributions of amphibians and the fungus pathogen chytridiomycosis with future climate scenarios, the authors projected that areas most affected by chytridiomycosis would have little spatial overlap with areas most affected by global climate change and land-use change. Areas most affected by global climate change also have high land-use change impacts. The latter occurs in Africa, parts of northern South America and the Andes. Overall, the areas where the richest amphibian faunas occur will be disproportionately more affected by one or multiple threats than areas with low amphibian richness. (CS)
- December 5, 2011:
Where to search for missing amphibian species? Garcia-Rodriguez et al. recently provide a method that could help to find populations of such species, especially in countries that have undergone a severe decline in amphibian species, both in abundance and distribution, such as Costa Rica. By creating consensus climatic niche maps of the historic range of endangered species and their relictual (i.e. persistent) distributions, they considered an array of factors, such as conservation areas, potential presence of pathogens, collecting effort, annual precipitation, among others, resulting in geographic predictions that have matched well with recent discoveries of relictual populations. Their method could be influential on decision-making about the location of future protected areas, as well as where to target limited funds on the discovery of endangered species. (JW)
- November 28, 2011:
Rediscovery of the Hula Painted Frog (Discoglossus nigriventer) in northern Israel is a great and welcomed surprise. The species was declared extinct in 1996 and had not been observed since 1955. The only preserved specimens are the holotype, collected in 1940, a second specimen, collected in 1955, and two tadpoles. The initial discovery of the species included two specimens but the holotype ate its companion in captivity! On November 15, 2011, an adult was found by Yoram Malka, a warden at the Hula Nature Reserve. It was swabbed for future DNA studies and returned to its habitat. Discoglossus is otherwise restricted to southwestern Europe and northwestern Africa. The 6,000 hectare Hula wetlands region, an important resting area for migrating birds, was largely drained for malaria control in the 1950s, but happily the species survived in a 320 hectare remnant that became Israels first nature reserve in 1964. The discovery received great media attention, as is appropriate for an event heralded as biologically equivalent to the discovery of the Dead Sea Scrolls! (DW)
- November 21, 2011:
AmphibiaWeb has gone mobile! Good news for iPhone users as AmphibiaWeb now has a mobile app in beta, available for downloading from iTunes. Written by an enterprising AmphibiaWeb volunteer, it allows you to search for amphibian species by name or geography including your location and then view species descriptions, photos, and listen to calls. We are still adding functionality and refining the checklist feature that will generate a list of expected species by your location, so stay tuned for updates. Please let us know what you think; your feedback is valuable.
- November 14, 2011:
New studies of fungal genetics have implications for amphibians. Rhys Farrer et al.
report multiple deeply diverged lineages within the chytrid fungus Batrachochytrium dendrobatidis (Bd), which causes chytridiomycosis in amphibians. A specific hypervirulent lineage is associated with disease outbreaks on multiple continents. Perhaps human-mediated mixing of lineages of different geographic origin gave rise to the hypervirulent lineage. A second study by Suzanne Joneson et al. reports the complete genome of a related chytrid and compares it with Bd and 19 other fungal genomes, finding 1,974 Bd specific genes. Among these are a protease gene family that has emerged recently and may have implications for Bd pathogenicity. Probing genetic diversity of Bd and related fungi will likely be an important future direction for understanding the origin, spread, and pathogenicity of Bd. (DB & DW)
- November 7, 2011:
A major revision of South American poison frogs discusses controversies over the taxonomy of Dendrobates (in the broad sense, as previously on AmphibiaWeb) and supports division of the genus into seven genera, including a new genus Andinobates. The monograph by J.L. Brown , E. Twomey, A. Amézquita, M.B. De Souza, J.P. Caldwell, S. Lötters, R. Von May, P.R. Melo-Sampaio, D. Mejía-Vargas, P. Perez-Peña, M. Pepper, E.H. Poelman, M. Sanchez-Rodriguez, and K. Summers (A taxonomic revision of the Neotropical poison frog genus Ranitomeya (Amphibia: Dendrobatidae) 2011 Zootaxa 3083: 1-120) is profusely illustrated in color and includes extensive analyses of new and existing molecular data and a great deal of detail concerning the biology of these diminutive and spectacular species. (DW)
- October 31, 2011:
An extensive new phylogeny has been reconstructed for amphibians, based on over 2,800 species representing 86% of amphibian genera (Pyron and Wiens 2011). In comparison, the largest previous study on amphibian phylogenetics (Frost et al. 2006) used 522 species and data from less than half as many genes. Pyron and Wiens' supermatrix analysis supports many previous conclusions on amphibian familial taxonomy but also shows that some currently recognized families are non-monophyletic and recognizes a number of new families. See the latest issue of Molecular Phylogenetics and Evolution (November 2011) for more details. (KW)
- October 24, 2011:
Many studies using future climate models have shown the potential, and often severe, impact on species distributions. A recent one in Ecology Letters ( Early and Sax 2011) took a closer look at future climate projections specifically by tracking 15 western North American amphibian species distributions as predicted by future climate niche models and the likely corridors they would have to travel by 2100 to maintain their current environmental conditions. They found that even if future distributions were large, the intermediary decades of climactic fluctuations may still imperil species if range shifts are not continuous or too brief for low vagility species like amphibians. The authors raise important considerations for conservation efforts. (MK)
- October 17, 2011:
Female Strawberry Poison frogs, Dendrobates pumilio, can be aggressive to each other, and a new study by Meuche, Linsenmair, and Pröhl (2011) explains why. In the Hitoy Cerere Biological Reserve, Costa Rica, female frogs defend a core area against other intruding females. The core area was defined as 50% of observation points within the females home range. Females did not defend mates, oviposition or tadpole-rearing sites but seem to be defending spatially-limited food resources. Over 54% of all female-on-female agonistic behavior took place within individuals core areas, and most female feeding also occurred within this core. Aggressive behavior included jumping, clasping or wrestling. Defense of food is important to females both for survival and because food intake influences egg size. (CS)
- October 10, 2011:
Do you know about the
Bolivian Amphibian Initiative? This project focuses on both monitoring and conservation of Bolivias endangered amphibian species, notably in the high Andes. The Bolivian Amphibian Initiative provides training and community outreach as well as research opportunities and conservation actions such as a captive breeding program. This landlocked country holds at last count 240 amphibian species, a good fraction of them being endemic and threatened or endangered by several factors while many are simply data deficient. A lot of areas are poorly documented and monitoring is highly needed. This project, bringing together local people, park guards and biologists, is a remarkable initiative that addresses much needed attention and focus on Bolivian amphibian fauna. (JW)
- October 3, 2011:
In a recent study of wild frog populations, Savage and Zamudio demonstrate a genetic basis to survival following infection with the chytrid fungus Batrachochytrium dendrobatidis (Bd). When looking across the populations in their study, the authors found that having more genetic variation in immune (MHC) genes was positively related to an individuals ability to survive Bd infection. Within populations, one specific genetic variant was related to increased survival of individuals. Importantly, this one variant that confers resistance was found to be under positive selection. This demonstrates that natural populations of amphibians can evolve resistance to Bd. (DB)
- September 26, 2011:
The Western Ghats lines the entire western front of India, forming a mountainous rain shadow, and consequently, a recognized biodiversity hotspot. Even so, the full extent of amphibian biodiversity may have just started to be fully known as suggested by the 24 new amphibian species formally described in 2011 so far, including a new caecilian, one Polypedates frog, nine Raorchestes frogs, one Leptobrachium frog, and now S.D. Biju and colleagues reveal 12 new species of Nyctibatrachus, also known as Night frogs. In a taxonomic revision of the genus, they also report the rediscovery of three species thought to be extinct, a new morphological structure to diagnose this group, as well as new courtship and egg attendance behaviour for several of these species. In all, the genus Nyctibatrachus is recognised with 28 species in a paper that lays the groundwork for more detailed research for this genus and amphibians of the Ghats. (MK)
- September 19, 2011:
Barbourula kalimantanensis, the only frog known to lack lungs, lives in cool, clear, well-aerated, mainly headwater streams in mountains along the border of West and Central Kalimantan in a small area in the west-central island of Borneo. Strictly aquatic, its only relative is a congener restricted to three islands in the western Philippines. It is currently listed as Endangered by IUCN. Rachmayuningtyas et al. report new discoveries that extend the range and disclose some genetic diversification. Habitat is severely limited but the range now extends over an area of more than 5,000 sq km so the authors propose reclassifying the species as Vulnerable. They also propose that the species be treated as a flagship species for Bukit Baka Bukit Raya National Park, where several populations occur. Nevertheless, the severe habitat requirements of the species means that vigilance is necessary to prevent logging and stream degradation. (DW)
- September 12, 2011:
The severe wildfires in Texas has affected millions of people and wildlife in the state. The endangered Houston Toad, Bufo houstonensis, will likely suffer a severe setback as a result of fires in Bastrop County, near Austin, Texas. The area has had a combination of >75 days in the triple digits and virtually no rain in several months. The loblolly pine forest encompassing Bastrop State Park became a tinder box waiting to explode, and it did so during the past week of September. All but 50-100 acres of the 6,000-acre park has been burnt. The park and surrounding area are the residence of one of the largest populations of the toads, numbering about 2,000, and the fires may have devastated their numbers. Because Bufo houstonensis breeds in February and March when the first spring rains arrive, and is not active for most of the year, the fire's effect will be unknown until the next breeding season. (DC)
- September 5, 2011:
A reptile on AmphibiaWeb?! A little over 3 months old, the Global Amphibian Bioblitz has been steadily growing with now 11% of the worlds amphibian species observed by naturalists worldwide. With increasing coverage, it has inspired the Global Reptile Bioblitz, which aims to do the same for the worlds 9,413 species of reptiles. Help spread the word, increase awareness of our fascinating herpetofauna, and make your mark on the Global Amphibian and Reptile Bioblitzes! (MK)
- August 28, 2011:
Think this is a snake? Look again--it's a fanged frog (Limnonectes macrocephalus). Setiadi et al. (2011) report in the journal American Naturalist that a surprisingly diverse adaptive radiation of fanged frogs has taken place on the island of Sulawesi, Indonesia, over the relatively rapid time span of just 15 million years. Fanged frogs (genus Limnonectes) are the only frog group on Sulawesi that have successfully diversified, and body size corresponds strikingly with ecological niche. For instance, small-bodied fanged frogs are found in leaf litter near rivers and have evolved derived modes of reproduction (including viviparity) allowing them to invade terrestrial niches. A comparison with the anuran fauna of the Philippines reveals that the absence of competition (e.g., from the frog genus Platymantis) may have enabled the expansion of Limnonectes into many new niches on Sulawesi. (KW)
- August 22, 2011:
Chytridiomycosis has devastated many species of amphibians in Australia, Central America, Europe, and North America. However, few studies so far have examined the impact this fungal disease is having on Asian amphibians. Now Swei et al. (2011) report in a new PLoS One paper that chytridiomycosis infection prevalence patterns are strikingly different in Asia. The fungal pathogen Bd appears to be widely distributed in Asia (as previously predicted by niche modeling) but at very low prevalence. Also, in contrast to Central America and California, no evidence of a moving epidemic wave has been found, and mass amphibian die-offs do not appear to have occurred in Asia. The implications are that Bd may be either endemic at low prevalence, newly emerging (possibly in the Philippines), or unable to fully invade Asian amphibians. (KW)
- August 15, 2011:
Natural hybridization between species is an important evolutionary mechanism for creation as well as extinction or local extirpation of lineages. The formation, maintenance, and consequence of hybrid zones between species is both an evolutionary and ecological study. Such a study (Hauswaldt et al 2011) on the natural hybridization between the ancient sister lineages of Salamandrina perspicillata and Salamandrina terdigitata, endemic species of the Italian Appennine peninsula, show extensive, ongoing hybridization (up to 80% of the contact population). These two species were only recently distinguished from each other (2005) and little is known of their natural history, which confounds how these species are able to maintain their observed molecular distinctions as species and leads to questions of whether ecological mechanisms may also be in play. (MK)
- August 8, 2011:
Many species of Poison Frogs (Dendrobatidae) are brightly colored and distasteful to predators. A predator, such as a snake or bird, that grabs a poison frog such as Ameerega bilinguis (above) will spit it out and eventually learns to avoid frogs that resemble it. However, the "poison" frog Allobates zaparo (above), in the same family, is not distasteful. Instead, it is a mimic of the distasteful species Ameerega bilinguis. In this phenomenon, Batesian mimicry, the mimic is protected from predators who leave it alone because it resembles the foul-tasting species, called the model. Both model and mimic live in the same region of lowland rainforest in Ecuador. Although some other species of frogs are believed to be Batesian mimics, Allobates zaparo is the only species for which experiments have shown conclusively that predators cannot distinguish it from the distasteful species. Can you tell which species is harmful and which is harmless? (DC)
- August 1, 2011:
Unlike most papers on the deadly amphibian fungal disease caused by Batrachochytrium dendrobatidis, or Bd for short, a recent paper in Frontiers in Zoology evaluate epidemiological solutions to this global crisis, and thus shed hope on the conservation of amphibians. It is clear from their review of Bd pathogenesis that much has been rapidly learned of this organism, and therein lies many of the strategies. From in situ solutions of habitat alterations (e.g. ecosystem engineering) to bioaugmentation and possible immunization with vaccines, these are sophisticated but still untested solutions that nonetheless point to a brighter future. (MK)
- July 25, 2011:
How might montane amphibians respond to climatic warming? Gifford and Kozak studied two southern Appalachian salamanders, Plethodon teyahalee and P. jordani to determine what sets their elevational range limits. Plethodon jordani is intolerant to warmth and is restricted to uplands. In contrast, P. teyahalee, which replaces P. jordani at lower elevations, would thrive at higher elevations, but its range is limited by apparent competition with P. jordani. Ancestors of eastern Plethodon may have been upland forms and phylogenetic conservatism expressed in physiological traits may explain why so many of them are restricted to upland sites. More generally, range limits, often considered to be determined mainly by competition, may instead be determined more frequently by organismal traits. (DW)
- July 18, 2011:
Good news: One of the Lost Amphibian species on Conservation International's top ten list has been rediscovered: Ansonia latidisca, the Borneo rainbow toad. This spectacular arboreal toad had not been seen since a few specimens were collected in 1924. It was rediscovered in a remote area of Malaysian Borneo, after months of searching by a team led by Dr. Indraneil Das of Universiti Malaysia Sarawak (UNIMAS). (KW)
- July 11, 2011:
The Global Amphibian Bioblitz is a recently launched citizen science project, which aspires to observe every species of amphibian around the world. Such ambition requires the efforts of naturalists everywhere in collaboration with AmphibiaWeb, the Smithsonian Conservation Biology Institute, Center for Biological Diversity, the IUCN/SSC Amphibian Specialist Group, and Amphibian Ark. The project website and database is powered by the iNaturalist site where anyone can sign up and participate. Initiated on May 25th, the GAB has already logged in 589 species out of 6,823 known amphibians, 8 % of all species, representing 84 % of amphibian families. Keep up with the progress of the global effort and log in to participate as an observer or communicate with others at the Global Amphibian Bioblitz. (MK)
- July 4, 2011:
Dorsal crests of male newts (family Salamandridae) are particularly conspicuous during breeding season and their origin may be related to the complex courtship behaviors of these salamanders. In a new analysis that combines information on evolutionary relationships with data on morphology as well as behaviors, Wiens and colleagues reveal a complex relationship among these traits. The various dorsal crest traits that characterize mature male newts have been lost repeatedly. The evolution of the dorsal crest may be related to certain specific behaviors such as fanning and whipping of the tail. Species with higher numbers of crest-related traits also have larger repertoires of courtship behaviors. (DCB)
- June 27, 2011: Hellbenders (Cryptobranchus alleganiensis), known to be rather sedentary sit-and-wait predators, were found in a recent study in Indiana ( Burgmeier et al. 2011) to move further and have larger home ranges than previously thought. More movement occurred during the summer, which is their breeding season, than in the fall. Home ranges were on average 1545 m2, but during the fall home ranges decreased to 644 m2. Hellbenders preferred gravel substrate in flowing water (runs), and also pools over riffles. They used medium to larger sized boulders more frequently than expected. Larger shelter rocks were used in non-summer months with the average movement between frequently-used rocks about 28 m. (CS)
- June 20, 2011: Poison frogs (Dendrobatidae) are famous for their bright coloration. It is well known the most brightly colored species are protected by distasteful skin alkaloids. A predator that attacks one of these frogs will be unlikely do so again. This combination of defense and conspicuousness is called aposematism. A second and recently documented function of brightness is that females preferentially choose males based on their brightness and color. Now a third advantage is shown. In the strawberry poison frog, Dendrobates pumilio, the bright coloration functionsin male-male competition. Males of this species set up territories that they defend vigorously against other males. When defending his territory, a bright-colored male preferentially approaches brighter (as opposed to dull) intruders, does so more quickly, and directs more calls to these rivals. For these frogs, there are many advantages to being obvious. (DC)
- June 13, 2011: The enigmatic Vegas Valley Leopard Frog (Rana fisheri) once thrived in springs and associated creeks and ponds in the Vegas Valley of southern Nevada before urbanization destroyed its habitat. By 1942, the species was thought to be extinct. Using ancient DNA methods with frogs fixed in ethanol in 1915 and preserved at the California Academy of Sciences, Hekkala and colleagues (2011, Biological Conservation) have shown that samples of R. fisheri cluster within the northwestern clade (of two clades currently assigned to Rana chiricahuensis), and they have assigned members of that clade (mainly from the Mogollon Rim region) to R. fisheri. The status of the second clade, currently R. chiricahuensis, is now in question, especially important given recent focus on conservation efforts. (DW)
- June 6, 2011: Tropical amphibians in pristine forests are more likely to be infected by the fungal chytrid pathogen, Batrachochytium dendrobatidis (Bd), than amphibians in deforested areas of the tropics, according to research by Becker and Zamudio (2011), published in PNAS. (JG)
- May 30, 2011: Although Sri Lanka and India were connected by land bridges several times, most recently at about 10,000 years ago, their faunas do not entirely overlap and clade-level endemism is present. Reinforcing this observation, Meegaskumbura et al. have distinguished a new genus of foam-nesting rhacophorid treefrogs endemic to Sri Lanka. The new genus Taruga contains three species (Taruga fastigo, T. eques, and T. longinasus), all with restricted distributions mainly in Sri Lanka's forested highlands. All three species were formerly considered to be in Polypedates, but both Taruga adults and tadpoles can be distinguished morphologically and molecularly from the more widely ranging Sri Lankan Polypedates species (P. cruciger, P. maculatus). (KW)
- May 22, 2011: Good news for frogs: Five endemic African frog species, not seen since their descriptions over half a century ago, have been rediscovered in the mountains of eastern Democratic Republic of Congo. A team led by Eli Greenbaum of the University of Texas El Paso found Arthroleptis pyrrhoscelis, Chrysobatrachus cupreonitens, Hyperolius chrysogaster, Hyperolius leucotaenius, and Phrynobatrachus asper on Itombwe Plateau and in Kahuzi-Biega National Park. The remote Itombwe Plateau is one of the most biologically distinctive regions in Africa, with high levels of amphibian and bird endemism and a unique gorilla subspecies, but eastern Congo forest habitats are increasingly being destroyed for agricultural use. (KW)
- May 16, 2011:
In contrast to the limited range of morphological variation in adult frogs, tadpoles have diversified into an impressive array of ecomorphs. In a new recent paper, Roelants, Haas and Bossuyt (PNAS May 9, 2011) used a molecular phylogeny to show that the greatest expansion in morphological types were coincident with the basal anuran radiation. Subsequently, morphospace evolution slowed; extensive morphological evolution associated with the neobatrachian radiation featured homoplasy rather than innovation. (DW)
- May 9, 2011:
Atelopus are the brightly colored Neotropical Harlequin toads. This clade (Bufonidae) has suffered drastic population declines and extinctions of more than half of the 100 species (including A. ignescens pictured above). A phylogeny of approximately 20 species, reveals two patterns (Lötters et al. 2011 Systematics and Biodiversity 9(1):45-5). First, the variable colors and patterns of the species have confounded species delimitations. Second, the traditional delineation into lowland, gracile species and montane, robust species, does not conform to phylogeny. Rather, the group matches biogeography, with a northern Andean-Chocoan-Central American clade, and a southern Andean-Amazonian-Guianan clade. (DC)
- May 2, 2011:
Natural hybridization among recent species has been viewed traditionally as a homogenizing force, but new research has revealed a possible role for interspecific gene flow in facilitating species radiations. Natural hybridization can contribute to radiations by introducing novel genes or reshuffling existing genetic variation among diverging species. Fontenot et al. (2011) examined nuclear and mitochondrial variation in the Bufo (Anaxyrus) americanus group. Their phylogenies from nuclear and mitochondrial data were discordant, due to recent and/or ongoing natural hybridization events and high levels of gene flow between sympatric populations. This group may have benefited from genetic admixtures that have occurred since recent glaciations. (KW)
- April 25, 2011:
In a forthcoming study, Vieites et al. analyze relationships among the major lineages of the most diverse family of salamanders, the Plethodontidae, using DNA sequence data from nuclear genes and complete mitochondrial genomes. They find that the European and Asian plethodontids, Hydromantes and Karsenia, are sister taxa and part of a large clade of North American salamanders. The four-toed salamander, Hemidactylium scutatum, is found to be part of a lineage containing the slender salamanders (Batrachoseps) from western North America and the diverse tropical plethodontid salamanders of Central and South America. Based on these analyses, the authors proposed a revised subfamily taxonomy for the Plethodontidae. (DB)
- April 18, 2011:
In some caecilian species, larvae tear off and consume their mother's skin for nutrition. Now, for the first time, it has been reported that this may occur in an anuran species (Rabb's treefrog, Ecnomiohyla rabborum, from Panama). In an unusual twist, it may be the male parent providing nutrition; tadpoles develop in water-containing treeholes and field observations indicate that the tadpoles appear to rasp the epidermis of the father frog when he immerses his body among them. However, this hypothesis is unlikely ever to be formally tested. Chytridiomycosis has swept through and devastated the amphibian community at the type locality for this species, and only one lone male is known to survive in captivity. Read Mendelson's (2011) essay in Herpetological Review for more. (KW)
- April 11, 2011:
It's not easy being green...unless you're a spotted salamander (Ambystoma maculatum) embryo. Kerney et al. (2011) have documented the first known case of endosymbiosis between a vertebrate and an alga. The green algae are found not only in the jelly coat of the egg but also incorporated into the salamander's embryonic and larval tissues. Although the symbiotic relationship is relatively short-lived, with embedded algal cells diminishing in number as the salamander larva develops, this finding opens new avenues of inquiry into intracellular symbiosis in vertebrates. (MK)
- April 4, 2011:
The Neotropics (tropical areas in Central and South America) contain half of the Earth's remaining rain forests and the largest diversity of amphibian species of any region. A recent study reconstructed the biogeography of a highly diverse group of Neotropical frogs, the poison frog clade (Dendrobatidae). The study rejected an Amazonian center-of-origin for this group of frogs, and instead, concluded that a significant percentage of dendrobatid diversity in Amazonia resulted from repeated immigrations (mostly from the Andes Mountains), with radiations at <10.0 million years ago (MYA), rather than in situ diversification. The study also concluded that dendrobatid frog species in the Andes, Venezuelan Highlands, and Guiana Shield have undergone extended in situ diversification at near a constant rate since the Oligocene. (VV)
- March 28, 2011:
Developmental biologists seeking to understand the genetic and embryological underpinnings for the evolution of the basic shapes and forms used to recognize major groups have long been interested in the Hox genes that control the head-tail axis and major body segments in vertebrates and invertebrates alike. Recently, Mannaert and colleagues (2010) compared Hox gene clusters across amphibians and other vertebrates, and discovered that caecilians had an enlarged section of Hox genes, three times larger than in mammals, which more closely resembled those sections of the gene clusters in coelacanths and snakes, although unique in configuration. Whether the specific gene loss and gain is responsible for limblessness in caecilians remains unclear, but it certainly shows there is more vertebrate variation in Hox genes than previously thought. (MK)
- March 21, 2011:
A special double issue of the journal Diseases of Aquatic Organisms is solely devoted to "Chytridiomycosis: an emerging disease". The
double issue (Vol. 92, numbers 2-3: 89 - 260) contains a foreword (by AD Hyatt, R Speare, AA Cunningham, and C Carey) and twenty articles on diverse topics by many authors. Excerpt from the Foreword: Chytridiomycosis "is arguably the most significant recorded infectious disease of any vertebrate class. The disease is reducing amphibian biodiversity across most continents and regions of the world, affecting the resilience of surviving populations and driving multiple species to extinction". The articles range from general overviews (e.g. JP Collins on Amphibian declines and extinction: what we know and what we need to learn) to technical studies of the chytrid organism itself or its effects on particular species (such as Leiopelma archeyi) and are global in scope. (DW)
- March 14, 2011:
Manu National Park in southern Peru is a hotspot for amphibian biodiversity, encompassing habitat from lowland tropical rainforest to high-elevation Andean cloudforest. While lowland amphibian species such as this tiger-striped leaf frog Phyllomedusa tomopterna are doing well, chytridiomycosis is wreaking havoc at higher elevations in Manu. Work by Catenazzi and colleagues has documented a dramatic decrease in species richness and abundance for Manu high-elevation anuran species over a 10-year period, particularly in stream-breeding amphibians. Their data support the hypothesis that the pathogenic chytrid fungus Bd is moving southward in a wave along the Andean cordilleras. (KW)
- March 6, 2011:
The giant toads of the forests of West and Central Africa are often feared by local people but also play a role in traditional medicine and lore, including that these toads give birth to the rainbow. Now
Barej and colleagues have revised this large, charismatic species by recognizing three distinct lineages. Distinct subspecies of Bufo (Amietophrynus) superciliaris are restricted, respectively, to the Lower Guinean forests and the Upper Guinean forests centered around Ivory Coast and Liberia. In addition, a new species, A. channingi, is described that is so far known only from the war-torn eastern Democratic Republic of Congo, including the Itombwe Plateau, already known for its endemic amphibian species. (DB)
- February 27, 2011:
Kaiser et al. (2011) found that anthropogenic noise decreases both the long- and short-term duration in which male frogs are at the breeding chorus. This is likely to decrease reproductive success because females join the choruses later at night. So, males and females overlap less at the breeding pond. This phenomenon potentially influences population dynamics and may contribute to anuran declines. (DC)
- February 20, 2011:
A five-month long
Search for Lost Frogs
organized by Conservation International (CI) and
the IUCN Amphibian Specialist Group (ASG),
with support from Global Wildlife Conservation (GWC),
has resulted in the re-discovery of only four species from a "Top 100" list. An additional eleven species
that have not been seen for many years were also found.
The search involved 126 researchers in 21 countries on 5 continents. (JG)
- February 13, 2011:
Mexican Spadefoot toads (Spea multiplicata) have evolved interesting adaptations for living in North American deserts. Some tadpoles of this species become carnivores, or even cannibalistic, preying on anostracan fairy shrimp and other tadpoles that share their ever diminishing ponds. Whether S. multiplicata tadpoles become carnivorous depends on their mothers size (Martin and Pfennig 2010). Larger females invest in larger eggs, which become larger tadpoles, and these tadpoles are faster at catching shrimp and more likely to become carnivores. Smaller mothers produce smaller offspring, which become omnivorous tadpoles. Therefore, maternal effects may reinforce character displacement and population divergence in Spadefoot toads and promote the evolution of novel phenotypes. (CS)
- February 6, 2011:
The fungal chytrid pathogen, Batrachochytium dendrobatidis (Bd), is reaping havoc on the world's amphibians causing hundreds of species to go extinct. Most of the attention has been focused on highly susceptible amphibians, yet hundreds of other species appear to become infected with little apparent effect. Whether there are substantial sublethal effects of Bd infection remains unknown. The authors of a currently published story (Han et al. 2011) examined the effects of Bd-sublethal effects on antipredator behavior of tadpoles from four species of western North American amphibians. They found that sub-lethal exposure to Bd can alter fundamental anti-predator behavior in Bufo boreas and suggest that it could increase predation risk in Bd-infected tadpoles. (VV)
- January 31, 2011:
Introduced species are a well-known phenomena in Florida. A recent phylogenetic analysis of two presumably recent introductions, the Greenhouse frog (Eleutherodactylus planirostris) and the Cuban treefrog (Osteopilus septentrionalis), show different patterns of nucleotide sequences compared to populations from their native range (Heinicke et al 2011). The study pinpoints E. planirostris emerging from a single colonization event from western Cuba to the Florida Keys, while O. septentrionalis is from two or more Cuban sites. Divergence times between Cuban and Floridian populations appear to be early enough for E. planirostris to be coincident with the formation of the Florida Keys, while the Cuban treefrog introduction likely was human mediated. The analysis contrasts a longterm resident from a more recent introduction, clarifying their dispersal patterns. (MK)
- January 22, 2011:
In 1985, slightly more than 4,000 species of amphibians were recognized. Today AmphibiaWeb recognizes 6,785 species. The vast majority of those named since 1985 are new discoveries. In 2010, 206 species were added to the AmphibiaWeb database, the largest number added since 2005. Although most of these were tropical frogs, the total also includes 25 salamanders (mostly in the tropics) and 5 caecilians. The rate of increase in numbers of new species continues to be high (3.13% in 2010, a surprising 59% since 1985), with no indication that the rate of discovery and description is slowing. (DW)
- January 17, 2011:
Although the amphibian chytrid fungal pathogen Batrachochytrium dendrobatidis (Bd) has devastated amphibian populations in many parts of the world, and initially it was thought that the pathogen might have spread through global trade of infected African pipid frogs, the prevalence and distribution of this pathogen in Africa is still not well known. Gabon has now been added to the list of countries in which Bd is widely prevalent. See Bell et al. (2011) in the journal EcoHealth for more details. (KW)
- January 10, 2011:
Vampire tadpole? A new species of rhacophorid treefrog from Vietnam, just described in Zootaxa by Rowley et al. (2010), has an unusual tadpole. Larval Rhacophorus vampyrus have a pair of black "fangs" (keratinized hooks) on the lower labium that curve away from the reduced mouthparts. To view a photo of the tadpole, go here. This species deposits its eggs in foam nests within small treeholes. (KW)
- December 26, 2010:
Climate change may affect many aspects of an animal's life, including breeding biology. In a recent paper, Brian Todd and colleagues found that four of ten amphibian species studied over a 30-year period have shifted the date when they arrive at their breeding site. Two autumn-breeding species and two winter-breeding species using the same wetland were found to have shifted breeding migration dates. Significantly, the onset of breeding in these species now coincides, whereas it had previously been separated by several months, and suggests that climate change can affect interactions among species. This change in reproductive timing is one of the largest reported to date and coincides with an increase in overnight air temperatures over the past 30 years. (DW)
- December 16, 2010:
Do you use AmphibiaWeb for mapping amphibians, checking out new species, getting titles of new amphibian decline papers (see Latest Papers button), scoping out Species of the Week, reading about the factors contributing to amphibian declines, finding out more about the natural history of a particular species, searching for all the caeciliids in India, or showing your herpetology class that you can see photos of every frog family simultaneously with our taxonomic photo browser?
Please consider including AmphibiaWeb in your holiday gift-giving plans!
- December 9, 2010:
IUCN has created a wiki page for the Amphibian Red List Authority, which has now been made available for viewing by the broader herpetological community. If you are interested in finding out more about how IUCN conducts its amphibian conservation assessments, this wiki is a very useful collection of resources. (KW)
- December 2, 2010:
Although the frog's jump is among the most familiar of all modes of locomotion in vertebrates, much remains unknown about the diversity and evolution of locomotion in frogs. Reilly and Jorgensen (2010), in a new Journal of Morphology paper, have used detailed anatomical studies (including x-rays and micro-CT imaging) to more thoroughly document the diversity of frog pelvic anatomy. They find that anatomy associated with walking and hopping is common among the earliest diverging lineages of living frogs. In contrast, specialized anatomy associated with long-distance jumping evolved multiple times and is found only within neobatrachian frogs.
To see one of Reilly and Jorgensen's CT images (the pelvis of Ptychadena anchietae, an African ranid that is a long-jumping frog), view the AmphibiaWeb Facebook page. (DCB)
- November 29, 2010:
Alkaloid skin toxins have now been reported from two species of Cuban frogs in the family Eleutherodactylidae. This is the first eleutherodactylid lineage known to be toxic. Rodríguez et al. (2010) show that Eleutherodactylus iberia (and to a lesser degree, E. orientalis) have lipid-soluble alkaloid skin toxins (pumiliotoxins and indolizidines). As is the case for other toxic lineages such as dendrobatid and mantelline frogs, the skin alkaloids are thought to be sequestered from dietary sources (mites).
Both Cuban species are miniaturized; in particular, Eleutherodactylus iberia is the smallest known frog in the Northern Hemisphere and one of the smallest in the world. Miniaturization, along with dietary specialization in tiny prey (mites and ants), is thought to have preceded the evolution of alkaloid sequestration, aposematic coloration and diurnality in anuran taxa.(KW)
- November 22, 2010:
Find your amphibian species of interest! AmphibiaWeb is now generating maps for newly described species, as well as for previously known species. For new species, we map the type locality. Maps can be accessed via the link at the top of all species account pages: "View distribution map using BerkeleyMapper." See the account for the new Nicaraguan salamander species Oedipina nica, for an example. Type localities have been mapped for all new species added to AmphibiaWeb through September 28, 2010, and will be updated on a regular basis; these maps will also be shared with IUCN/GAA. (KW)
- November 18, 2010:
Not only Haiti's people but Haiti's wildlife face great challenges. Haiti has the highest percentage of threatened amphibians, of any country: 92% of the amphibian species that have been assessed are in danger. Half of Haitian amphibians are endemics (28 of 57 species). Habitat loss presents the gravest threat. Only about 1% of the original forest now remaining in Haiti; what remains is rapidly being cut down for charcoal to be used in cooking fires. A new species-rescue initiative to save Haiti's frogs is now underway. Dr. Blair Hedges of Penn State has started a captive-breeding program for ten critically endangered species of Haitian frogs, in collaboration with the Philadelphia Zoo. Hedges' lab has also established a cryobanking program. (KW)
- November 17, 2010:
Conservation International's search for "Lost Frogs" has led to the discovery of three new frog species in Colombia (not yet formally described). Although the team did not find the Mesopotamia beaked toad (Rhamphophryne [Rhinella] rostrata, probably extinct), they did come across a tiny new species of beaked toad (genus Bufo [Rhinella]), a new rocket frog (genus Silverstoneia, family Dendrobatidae), and a new montane toad with vivid ruby eyes (genus unknown). See the BBC story and the CI press release for photographs. (KW)
- November 11, 2010:
AmphibiaWeb has reached a milestone! We now have 20,000 amphibian photos, representing over half of all extant amphibian species. Many thanks to all of our talented photographers, and thanks also to CalPhotos for hosting the images. Congratulations to Dave Blackburn of KU for submitting the 20,000th photo, especially since it is of a species just described (Arthroleptis palava). In celebration, we would also like to announce a new feature: AmphibiaWeb now has a taxonomically organized photo browser. You can access this using the "Search the Database" button on the home page or on the left of any other page, and then choose the blue tab "Browse Photos". Check it out! (KW)
- November 8, 2010:
Through recent efforts such as the Global Amphibian Assessment, we have a nearly comprehensive view of amphibian diversity, distributions, and threats. But what about the people studying and describing amphibian species? In a new BioScience paper, Rodrigues et al. (2010) document the distributions, regional expertise, and ages of active amphibian taxonomists. Europe and North and Central America are net exporters of taxonomic knowledge to many other regions. South America has a relatively young population of amphibian taxonomists, compared to Asia. Africa is most in need of local amphibian taxonomic expertise. This work highlights the need for taxonomic training and particularly increased local expertise throughout many regions of high amphibian diversity. (MK)
- November 4, 2010:
A new initiative to find missing amphibian species in India has been announced: Lost! Amphibians of India.
Scientists from the University of New Delhi, Conservation International, the IUCN/SSC Amphibian Specialist Group, the Natural History Museum (London), the Wildlife Conservation Society, and Global Wildlife Conservation are on the hunt for 47 amphibian species not seen in India for many years. In tandem, a new partnership (the Western Ghats Network of Protected Areas for Threatened Amphibians, or WNPATA) has been created to protect amphibian habitat in the Western Ghats, one of the ten richest hotspots for biodiversity in the world. (KW)
- November 1, 2010:
In comparison to other families of frogs found in Africa, the family Brevicipitidae remains poorly known. In a new study, Loader et al. (2010) describe three new species of Callulina, a genus restricted to montane forests of East Africa. Unlike most brevicipitids which are burrowers, species of this enigmatic genus are often found climbing on vegetation. For nearly a century, there was only one recognized species of Callulina, but since 2004 five more have been described. Each species appears to have a very localized distribution. In combination with ongoing loss of forests in the region, their restricted ranges suggest that conservation measures may be needed to protect these unusual frogs. This study also further highlights the northernmost Eastern Arc Mountains as a place of high species-level endemism. (DCB)
- October 25, 2010:
Frogs have teeth, but did you know that tadpoles do too? Tadpole teeth are not true teeth but are instead independently evolved and developed structures made up of keratin (called labial teeth, keratodonts, or denticles). Vera Candioti and Altig (2010) investigate whether shape diversity among tadpole keratodonts might be explained by shared evolutionary histories and/or by being members of similar ecological guilds. The authors find that 35% of shape variation can be explained by common ancestry and that suctorial tadpoles have much broader keratodonts than other ecological guilds, possibly for better grasping the substrates in habitats such as fast-moving streams. The authors do not find clear relationships between keratodont shape and diet. This study will serve as a baseline for future work on understanding the relationship between the form and function of these uniquely tadpole structures. (DCB)
- October 14, 2010:
During metamorphosis, the skeletons of amphibians change dramatically. In a recent study published in the Journal of Zoological Systematics and Evolutionary Research, Weisbecker and Mitgutsch (2010) investigate the degree to which these patterns of skeletal change are conserved across the evolutionary history of frogs. After analyzing a compilation of data on ossification sequences from past literature, the authors find relatively little conservation of developmental patterns. The authors include data for both miniature species and those with derived life histories (such as direct development), but, importantly, do not find a clear relationship between patterns of ossification sequence and these important organismal traits. (DCB)
- October 11, 2010:
What factors influence whether frogs can recolonize a site after a local extinction? Landscape genetics focuses mainly on how habitat features (topographic complexity) between sites can influence gene flow. However, other factors influence frog populations such as distance between suitable breeding sites and local factors such as whether predatory fish are present. In a new Molecular Ecology paper, Murphy et al. (2010) use the novel approach of gravity modelling (previously used only in economic geography and transportation) to capture the complexity of factors affecting Rana luteiventris (Columbia spotted frog) gene flow. (KW)
- October 5, 2010:
Chytridiomycosis is in the NY Times today, with an article on Vance Vredenburg's work. He is trying to save the last of the Sierra Nevada yellow-legged frogs (Rana sierrae) left in the wild from chytridiomycosis, by using the novel method of bacterial bioaugmentation. Check out both the print article and the audio slideshow. (KW)
- September 27, 2010:
Good news: A rare cave-dwelling salamander species has been rediscovered in Mexico after 73 years. Despite extensive searches Chiropterotriton mosaueri, the cave splayfoot salamander, had not been sighted since it was discovered in 1937 (it was formally described in 1941) and was feared to be extinct. AmphibiaWeb congratulates Dr. Sean Rovito (a postdoctoral fellow at UNAM) on finding this species, and Conservation International for its support of this expedition as part of its campaign to "Search for the Lost Frogs."
Even better news: Last week three different CI expeditions reported success in finding their amphibian targets. Congratulations to all! (KW)
- September 20, 2010:
Ontogenetic color change, where the juvenile has a very different coloration from the adult, is unusual in frogs. In Stumpffia be, a new microhylid species from Madagascar, the juvenile has bright blue spots on a dark background, in contrast to the relatively plain light brown adult. This species is part of a newly described radiation of cophyline microhylids that apparently live mainly in karstic limestone caves (a new habitat for cophylines) and have re-evolved larger body size from miniaturized ancestors. See Köhler et al. (2010) in the latest issue of the Journal of Zoology. (KW)
- September 13, 2010: Are Neotropical direct-developing frog populations that have survived Bd epidemics still at risk, and what factors influence survival? Direct-developing species are the most likely to survive in Neotropical highland forests, since they do not breed in water and thus have less exposure to the aquatic infectious zoospore stage of Bd. In Puerto Rico, Bd arrived in the mid-1970s and has decimated many amphibian species. Longo and Burrowes (2010), in a new Ecohealth paper, show that populations which have somewhat recovered from Bd-influenced declines (Eleutherodactylus coqui and E. portoricensis) are still at risk. Infection persists in these populations and continues to hinder survivorship. Juveniles are more susceptible to infection than adults and carry higher infection loads, particularly at mid-elevations. (KW)
- September 6, 2010: Bolitoglossa jacksoni is one of the ten "most wanted" amphibians in Conservation International's 2010 campaign to search for lost amphibian species. This spectacular salamander is endemic to western Guatemala and has not been seen for 35 years. Only two individuals have ever been found (both in 1975). Although there is no habitat left at the type locality for B. jacksoni, a second potential locality will be explored. A team will go out for a short search in mid-October, and a second team will conduct a more extensive search in mid-November, led by Carlos Vasquez of the Museo de Historia Natural, Universidad de San Carlos de Guatemala. (KW)
- August 30, 2010:
A tiny new frog (the Old World's smallest) has been discovered living and breeding inside pitcher plants on Borneo. Adult males of Microhyla nepenthicola measure between 10.6 and 12.8 mm; new metamorphs measure just 3.5 mm on average (one-third the diameter of a pea). Although pitcher plants are carnivorous and consume insects that fall in, the tadpoles suffer no ill effects from developing inside the pitcher's digestive liquid. Das and Haas (2010) describe the new frog species in the journal Zootaxa. In September, Das will be leading one of a number of Conservation International teams searching for "lost" amphibian species that have not been seen in the wild for many years. (KW)
- August 23, 2010: The lethal amphibian disease chytridiomycosis has been detected in numerous regions worldwide, but sampling in Asia has been very patchy. The first report of chytridiomycosis in China has been published in the August issue of the journal EcoHealth, by Bai et al. (2010). Both native amphibian species and introduced bullfrogs (wild, farmed, and market-sold Rana catesbeiana) were found infected in Yunnan province. Bullfrogs are known carriers of the chytrid fungal pathogen Batrachochytrium dendrobatidis and are widely bred for both domestic and international consumption on frog farms in China. The authors call for strict regulations on bullfrog breeding and transport in China, and point out the urgent need for monitoring of native Chinese amphibians for chytridiomycosis. (KW)
- August 16, 2010: What is the longest-lived amphibian and what traits predict longevity? Generally body size is tied to longevity, as larger animals such as elephants tend to have longer lifespans. Now the neotenic cave-dwelling salamander Proteus anguinus (known as the olm) has proven the exception, with a body mass of just 15-20 g (less than 3/4 ounce) but a predicted maximum lifespan of over a century. A new paper by Voituron et al. (2010) has analyzed over 50 years' worth of weekly records from a 400-animal captive breeding colony in the French Pyrenees. The average adult olm lifespan was 68.5 years; sexual maturity was attained at 15.6 years, on average. In contrast, the next longest-lived amphibian is the Japanese giant salamander (Andrias japonicus), weighing over 30 kg but with a maximum lifespan of only 52 years in captivity. (KW)
- August 13, 2010: Why did a rare, critically endangered African frog species suddenly die in high numbers on Mount Oku, Cameroon? Is a new, unknown pathogen responsible, or could it be due to lake acidification? In analyzing specimens of dead Xenopus longipes from a 2006 mass mortality event, Blackburn et al. (2010) found no evidence of chytrid infection or ranaviruses. No other amphibian species in Lake Oku, or in nearby watersheds, were observed to suffer mass mortality in the same time period. This paper serves as a reminder that amphibians face many threats and that chytridiomycosis is not the only suspect in enigmatic amphibian declines. Xenopus longipes is found solely in a single high-altitude crater lake on Mount Oku in Cameroon. This species is one of only two vertebrate species known to be dodecaploid (12n). (KW)
- August 11, 2010:
Intracellular symbiosis with photosynthetic organisms has long been documented for invertebrates, such as corals, but was thought impossible for vertebrates. Now a "solar-powered salamander," the first vertebrate to harbor a photosynthetic symbiont inside its cells, has been reported at the recent Ninth International Congress of Vertebrate Morphology in Uruguay.
Embryos of spotted salamanders (Ambystoma maculatum) have been found to contain many single-celled algae, of the species Oophila amblystomatis. Previously it had been thought that vertebrates were not capable of harboring intracellular symbionts due to the vertebrate adaptive immune system, which is responsible for recognizing and destroying "non-self" biological material. For a commentary , see Petherick (2010) in Nature. (KW)
- August 7, 2010: Can tectonic activity be traced with frogs? What can spiny frogs with Popeye-like forearms and sandpaper-like chests, living in cascading Asian mountain streams, tell us about the formation of the Himalayas? Geologists have long debated how the India-Asia collision led to the rise of the Himalayas and the Tibetan Plateau. Now biologists have used frog evolution within the tribe Paini (family Ranidae) to support a lesser-known model of the geological processes forming this area: the ramming of the Indian plate against Asia did not simply push up the Himalayas, but rather took place in a discontinuous series of northward jumps, first forming Southeast Asia, then pushing South China to the east, and then Central China to the northeast.
- August 2, 2010: A significant new collection of photos has been added to AmphibiaWeb by the University of Kansas Biodiversity Institute's Division of Herpetology. Of the 559 photos contributed, 328 species were not previously represented by photos in AmphibiaWeb, and 108 are holotypes. Thanks go especially to Dave Blackburn of KU for organizing the photo additions, to Bill Duellman for contributing so many of his own photos, and to Rafe Brown and Linda Trueb, as well as to the many KU photographers who have permitted their images to be publicly displayed on AmphibiaWeb. All copyrights are held by KU's Division of Herpetology. (KW)
- July 26, 2010: How deeply are amphibian communities affected by disease-driven declines and extinctions? How can species loss be quantified, particularly in tropical assemblages where many cryptic species are present? A new paper by Crawford et al. (2010) shows that the extensively studied amphibian community at El Copé, Panama, lost 41% of its phylogenetic diversity (30 of 74 species, in a 4 km2 area) after being devastated by a chytridiomycosis epidemic. Only 63 species had been described; DNA barcoding analysis of specimens revealed an additional 11 species, of which 5 had already been extirpated from the site. The loss of undescribed species is thought likely to be even higher in chytridiomycosis-affected areas not as well studied as El Copé. (KW)
- July 17, 2010: A new gliding frog has been described, Norhayati's Gliding Frog (Rhacophorus norhayatii) by Chan and Grismer (2010), from northwest and extreme southern Thailand and Peninsular Malaysia, and possibly Sumatra. AmphibiaWeb thanks Peter Janzen (who has contributed 687 photos in total) for the photo of this new species. To see a gliding frog in full glide, check out John Clare and Kurt Kunze's great photo in the R. reinwardtii species account. (KW)
- July 14, 2010:
Can amphibians in the wild be saved from the deadly fungal disease chytridiomycosis, using bioaugmentation of naturally occurring amphibian anti-fungal skin bacteria? This summer will be the first test, in California.
Mountain yellow-legged frogs (Rana muscosa and Rana sierrae) were once the most common vertebrates in the Sierra Nevada mountains but now are critically endangered, with many populations decimated by chytrid fungal infection. Wild frogs carry many different kinds of beneficial skin bacteria; at least one secretes a peptide called violacein that protects wild frogs against infection by the fungal pathogen Batrachochytrium dendrobatidis (Bd). Mountain yellow-legged frog populations that have little to no violacein-secreting bacteria succumb to Bd fungal infection and suffer mass mortality.
In the lab, inoculation with these naturally occurring beneficial bacteria (bioaugmentation) works to protect frogs (and salamanders) against fungal infection and death from chytridiomycosis. Vance Vredenburg of SFSU will lead a project in August 2010 to see whether bioaugmentation can help save some of the few remaining wild mountain yellow-legged frogs in Sequoia and King's Canyon National Park. See the July 2010 Scientific American article for more details. (KW)
- June 21, 2010: What distinguishes invasive amphibian species at the earliest stage of becoming invasive? Tingley et al. (2010) found that introduced amphibian species were biased taxonomically, with about 50% of introduced species coming from only 3 families: Hylidae, Ranidae, and Salamandridae. Introduced amphibian species were primarily from the Northern Hemisphere. As has been found for introduced mammals, birds, and fish, introduced amphibians had a broad geographic range and were associated with locations having high human density. Large species were more likely to have been transported intentionally, while smaller species were more likely to have been stowaways. Large species also tend to have life history traits associated with successful invasion (high fecundity, mobility, etc.). Ninety percent of caudate introductions were found to be intentional. (KW)
- June 19, 2010: Frogs get a leg up, as California's Fish & Game Commission voted 3-2 last month to uphold their recent decision to ban importation of non-native frogs (mainly bullfrogs, Rana catesbeiana) and turtles for use as food. Bullfrogs are voracious predators of native amphibian species (such as Bufo californicus) and are asymptomatic carriers of amphibian chytrid fungus (Bd), which has decimated native Californian species such as Rana sierrae. Nearly 1.6 million live ranid frogs are imported per year into the port of San Francisco alone; testing of marketplace-sold bullfrogs in SF, NY, and LA showed that 62% were infected with Bd (Schloegel et al. 2009). (KW)
- June 10, 2010: Can chytridiomycosis be treated in the wild? Researchers are trying different strategies to help frog populations most threatened by this fungal disease. In Mallorca, Spain, Jaime Bosch's group has been evacuating midwife toad tadpoles (Alytes obstetricans) and treating the tadpoles with antifungal baths in captivity. Bosch's group also attempted to eradicate the fungus in the tadpoles' home pond before releasing treated tadpoles back to the wild. In the Sierra Nevada mountains in California, Vance Vredenburg's group has been treating adult mountain yellow-legged frogs (Rana sierrae) in the wild, with short fungal baths once daily for a week. Initial results are mixed but promising. Read the news feature in the June 10th issue of Nature for more. (KW)
- May 31, 2010: Will frogs be able to successfully adapt to increasing global temperatures? A new study by Phillimore et al. (2010) suggests that projected increases in temperature may be more than the common frog Rana temporaria can handle, due to local adaptation. First spawning date for populations in southeast Britain will need to advance by about 21-39 days, but genetically influenced plasticity in spawning date may only allow an advance of 5-9 days. Gene flow northward from more southern populations already adapted to higher temperatures could help, but is unlikely due to the barriers of the English Channel and high urbanization. (DW)
- May 26, 2010: Red-eyed treefrog tadpoles (Agalychnis callidryas) can sense vibrations while the tadpoles are still in the eggs, and decide whether those vibrations are from predators or rainstorms. If the predator is a snake, the entire clutch will hatch synchronously to avoid being eaten. Now Caldwell et al. (2010) have shown that adult male red-eyed treefrogs also use vibrational signaling in male-male aggressive interactions, by shaking a branch rapidly with their hind legs. Substrate vibrations may be far more important in communication by arboreal vertebrates than previously realized. Check out the video in the original paper, and see also the commentary in ScienceNow. (KW)
- May 19, 2010:
If you missed hearing Dr. Vance Vredenburg discuss the amphibian decline crisis on this past week's NPR: Science Friday radio show, you can download the podcast here. Look for the May 14th segment titled "Is The Planet Facing A Mass Extinction?"
You'll also hear Dr. Barry Sinervo (UCSC) discuss his new research on lizard extinctions and climate change (just published in Science). Other participants are Dr. Tony Barnosky (UCB) and Dr. George Amato (AMNH). (KW)
- May 17, 2010: Does body shape affect locomotion or burrowing ability in caecilians? (in particular, are caecilians with more elongated bodies less efficient burrowers?) Apparently not as much as had previously been thought. Usually caecilians are active burrowers, but some prefer not to dig their own burrows; also, some species spend less time burrowing and more time foraging above ground, and a few are aquatic. Previous work showed that burrowing ability depended on using skin-vertebral attachment to generate force. In support, aquatic caecilians have lost skin-vertebral attachment, and it had been previously hypothesized that highly elongated species might also have lost that attachment. Herrel and Measey (2010) used X-ray video to examine caecilian locomotor mode on different substrates. The authors showed that both elongated and robust terrestrial caecilians were able to use internal concertina locomotion in soils, and that one robust species had higher skin-vertebral attachment but still preferred not to dig its own burrows. (KW)
- May 10, 2010: Why do some frogs (and populations) die from chytridiomycosis but not others? A pair of new long-term studies reveals that infection intensity is key; once a critical threshold of Bd fungal infection is reached, death ensues. Population extirpation is the most common outcome, but a few mountain yellow-legged frog (Rana sierrae and Rana muscosa) populations have survived in low numbers. Modeling shows that disease outcome at the population level (extirpation vs. persistence) can result solely from density-dependent host-pathogen dynamics, which may hold for other wildlife diseases as well. See Vredenburg et al. (2010) and Briggs et al. (2010) in PNAS for more. (KW)
- May 7, 2010: Captive breeding is a last resort to save endangered amphibians, but many species do not breed well under suboptimal captive conditions. A new protocol has been developed for artificially inducing ovulation and fertilization in captive amphibians by Trudeau et al. (2010), published in Reproductive Biology and Endocrinology. Injecting a combination of a gonadotropin-releasing hormone (GnRH) agonist and a dopamine antagonist successfully induced breeding in ranid, ceratophryid, and cycloramphid anurans. (KW)
- May 1, 2010: The first amphibian genome has been sequenced, for Xenopus tropicalis, enabling new insights into vertebrate evolution and the last common ancestor of tetrapods. Despite amphibians having diverged about 360 mya from mammals, birds, and reptiles, Hellsten et al. (2010) report that there is considerable sequence and gene order conservation. However, mammalian genomes have undergone far more chromosomal rearrangements. Many aspects of the vertebrate immune system are conserved, but unique to the amphibian genome are genes that code for antimicrobial peptides. See the latest issue of Science for more. (KW)
- April 26, 2010: Although mountain yellow-legged frogs (Rana muscosa) were once numerous in California, today fewer than 200 adults remain in the wild, with small populations in the San Gabriel, San Bernadino, and San Jacinto Mountains of Southern California. The San Diego Zoo Institute for Conservation Research has succeeded in getting a captive breeding program going for this species. Last week the first reintroduction to the wild took place, as biologists reintroduced about 500 eggs into deep permanent pools at the University of California Riversides James San Jacinto Mountains Reserve. (KW)
- April 18, 2010: A new paper from Acta Zoologica (Natale et al. 2010) reports that the tadpole of Ceratophrys ornata makes distress calls underwater. Although the paper claims that it is the first example of any larva communicating by sound underwater, and the first known of any vertebrate larva to make sounds, it is actually the second species known to do this (the first amphibian tadpole reported to make sounds underwater was Gephyromantis corvus, from Madagascar; see Glaw and Vences 1994). Ceratophrys ornata tadpoles are able to make the calls as early as three days after hatching, and can do so both in the water and out of the water. It is not known how other tadpoles perceive the calls, but while C. ornata larvae are carnivorous towards tadpoles of other species, they do not consume conspecific tadpoles. (The tadpole of Gephyromantis corvus is also carnivorous.) To hear the sound made by a Ceratophrys ornata tadpole, check out the two video clips in the associated BBC news story. (KW)
- April 15, 2010:
An amphibian documentary worth viewing: Why Frogs Call and Why We Should Listen (Ravenswood Media, 2009, 32 minutes). David McGowan's excellent footage succeeds in illustrating not only why frogs call and how frogs have evolved but also shows why amphibians are crucial biological indicators and illustrates the importance of wetlands to both frogs and humans. His compelling film serves to draw attention to the need for wetlands conservation as well as the consequences, both to amphibians and to humans, of continuing on our current path. Various clips from the film are available to view freely online, or the complete film can be purchased on DVD at midwestfrogs.com.
This film tells a compelling, tightly edited story, beginning with individual frogs calling in the wild and a discussion by researcher Carl Gerhardt (University of Missouri) of frog reproduction and how frog calls serve both to attract mates and repel competitors, sometimes within the same call. As Anne Maglia (MIssouri University of Science and Technology) then points out, frogs have had the ability to hear (and thus have probably been calling) for at least 190 million years, since the 190-million-year old fossil frog Notobatrachus has clearly visible middle ear bones (stapes). She reviews the fossil evidence of frog evolution and shows how frogs have evolved to become as specialized for jumping as birds are for flying. Amphibians that depend on water for breeding often mate in wetlands, which Robert Brodman (St. Joseph's College) points out are actually the most productive ecosystems on the planet, more so than tropical rainforest or coral reefs. However, wetlands are disappearing rapidly as they are drained and filled in for farmland or urban development.
Destruction of wetlands is not unique to the United States, as Caroline Aguti (University of Makerere) shows for Uganda; community education as to the value of wetlands can help. Wetlands are also threatened by chemicals; Val Beasley (University of Illinois) discusses the decline of a formerly common wetland-dependent frog species, the cricket frog (Acris crepitans), and the spikes he saw in reproductive abnormalities (intersex frogs, having both a testis and an ovary) coincident with the introduction of polychorinated biphenyls (PCBs) beginning in 1930. From 1946 to the 1960's, the frequency of intersex frogs showed yet another rise, this time coincident with the introduction of the pesticide DDT. Wetlands are under threat not only from pesticides but also from fertilizer runoff; Michael Lannoo (Indiana State University) illustrates how frog deformities are the end result in a chain that begins simply with more nutrients (fertilizer) coming into wetlands. As habitat loss pushes frogs into smaller and more altered habitat such as urban ditches, Karen Glennemeier (Audubon Society) shows how non-scientists can contribute by helping to monitor wetlands, using frog calls as the easiest way to assess biodiversity and thus the health of the wetlands. The film is brought to a close with some lovely images of wetlands and Val Beasley's positive comments on how wetlands have the ability to restore themselves if humans will only allow it to happen.
No film is perfect. It would have been nice if species were identified when they are seen in the film, but all seventeen individual species are identified in the individual clips, in a separate section of the DVD. The film touches on just a few aspects of the global amphibian decline crisis (though in fairness, it is a short film). It gives the impression that all amphibians depend on wetlands, which is not the case; many species lay terrestrial eggs (and a few give birth) and do not require bodies of water at all for breeding. Finally, although USGS presence/absence maps by counties (or other civil divisions, for certain states) are provided in a separate section of the DVD, there is no map legend for the color coding, and it is not clear how up-to-date or accurate these maps are, looking at the information provided.
Ravenswood has also made other DVDs and freely available "webumentaries" on topics such as cave biota, fishes in the Great Lakes, wildlife veterinary medicine, white nose syndrome in bats, and making ditches into better habitat. These can also be accessed via midwestfrogs.com. (KW)
- April 12, 2010: The Smithsonian is partnering with George Mason University to offer a course in amphibian monitoring and conservation next month (May 16-28), at the National Zoo's Conservation and Research Center in Virginia. The course will include lectures, lab and field exercises, and case studies. (KW)
- March 29, 2010: Can frogs with foam nests help make biofuels? The túngara frog (Engystomops pustulosus = Physalaemus pustulosus) creates long-lasting foam nests to help protect its tadpoles. Now a team of researchers has designed a novel artificial photosynthesis system suspended in a foam, using the túngara frog surfactant protein Ranaspumin-2. The system could produce up to 10-fold more biofuel per hectare than plants and could be used on rooftops and nonarable land (Wendell et al. 2010, in the journal Nano Letters). (KW)
- March 23, 2010:
Neurergus kaiseri, the Lorestan newt, has just been granted protection from international trade under CITES Appendix I, as of March 21, 2010. This striking salamander is known from only four streams in the remote Zagros Mountains, Iran. Population levels have declined significantly over the past 10 years, primarily due to overcollection for the pet trade. Fewer than 1,000 individuals are estimated to be left in the wild. Originally the CITES secretariat was planning to deny N. kaiseri protected status, but last-minute intervention by a number of biologists convinced delegates that this species should be included. (KW)
- March 20, 2010:
The California red-legged frog (Rana draytonii), made famous in the Mark Twain story "The Celebrated Jumping Frog of Calaveras County", has been extirpated from 99% of its Sierra Nevada range and is believed to be extinct in the Central Valley. Breeding populations remain along the coast, from San Mateo County to San Luis Obispo County. In 1996, it was declared a threatened species and over 4 million acres of habitat were designated as critical. However, in 2006 developers sued and 90% of that habitat designation was lost. Now that has been partially remedied, thanks to legal action by the Center for Biological Diversity. As of March 16, 2010, the U. S. Fish and Wildlife Service has designated 1.6 million acres of habitat as critical, and the agency has acknowledged that the earlier reduction was flawed because of political interference from the Bush Administration. (KW)
- March 15, 2010:
Did global warming contribute to the extinction of the golden toad (Bufo periglenes) by facilitating chytridiomycosis, as Pounds et al. (2006) suggested? A new PNAS paper by Anchukaitis and Evans (2010) says no. Using oxygen isotope data from old-growth trees, the authors reconstruct a century of climatic data for the Monteverde Cloud Forest Reserve, Costa Rica, and found no long-term warming or drying trend. Instead cloud forest ecology changes appear to have been driven by natural variability in the local climate (in particular, extreme dry periods associated with El Niño weather patterns) rather than by anthropogenic climate forcing. However, Pounds (who lives and works in Monteverde) commented to ScienceDaily that his own data from 40 years of rainfall and mist-cover measurements show that the weather is becoming more variable and more extreme. (KW)
- March 8, 2010:
Atrazine is the most common pesticide contaminant in ground, surface, and drinking water. It also is a potent endocrine disruptor at very low concentrations across vertebrate taxa. In a new PNAS paper, Hayes et al. (2010) showed that atrazine exposure during larval development at levels below the EPA drinking water standard can profoundly affect male Xenopus laevis sexual function and morphology. In the most severe cases, male frogs were completely feminized morphologically and behaviorally, producing eggs and mating with other males.
Amphibian declines may be due not only to disease and habitat loss, but to failure to reproduce (see the recent review by Hayes et al. 2010 in the Journal of Experimental Biology).
However, neither Syngenta (the manufacturer of atrazine) nor the EPA have so far accepted previous research, with the EPA saying only that sufficient data exists to form a hypothesis that atrazine can affect amphibian development, but that studies done up to 2007 have neither confirmed nor refuted such a hypothesis. See Renner (2008) for a commentary on reproducibility of atrazine experiments carried out using Xenopus. The EPA does state that it "has begun a reevaluation of atrazine's ecological effects, including potential effects on amphibians, based on data generated since 2007." (KW)
- March 3, 2010:
The California tiger salamander (Ambystoma californiense) has finally been granted protected status under the California Endangered Species Act, by a 3-2 vote of the California Fish and Game Commission on March 3, 2010. This native Californian species depends on ephemeral vernal pools for breeding, 95% of which have been lost in recent decades. (KW)
- March 1, 2010: The first known monogamous amphibian species (Dendrobates imitator) has been reported (Brown et al. 2010, in a paper to be published in the April issue of American Naturalist). Ecological factors appear to have driven the evolution of monogamy; this species breeds in tiny pools that lack food resources for tadpoles, necessitating parental care. A closely related species (D. variabilis) that breeds in larger pools is not monogamous and mothers of that species do not feed unfertilized eggs to tadpoles. (KW)
- February 18, 2010:
Do you use AmphibiaWeb in teaching your herpetology or undergraduate biology course? Please contact us and let us know the course you are teaching, the college or university, and how you are using AmphibiaWeb. Would you like to make a broader impact and have your students contribute species accounts to AmphibiaWeb, as part of your class? Students get authorship, and all accounts are edited before going public. Classes from U. C. Berkeley, Harvard, Black Hills State, San Francisco State University, and Cal State Stanislaus have written (or are writing) accounts.
- February 15, 2010:
Although chytridiomycosis has devastated many amphibian populations worldwide, it remains unclear whether the fungal pathogen responsible (Batrachochytrium dendrobatidis) is a new emerging pathogen or an endemic pathogen that has been exacerbated by environmental changes. A new paper by Walker et al. (2010) uses Bayesian modeling as well as genotyping to examine Bd occurrence on the Iberian peninsula, the site of the first European outbreak of chytridiomycosis. These authors conclude that Bd is a novel pathogen in Iberia but that heterogeneity in population response is due to both biotic and abiotic variables.
For a recent review of the ecology and impact of chytridiomycosis see Kilpatrick et al. (2009), in Trends in Ecology and Evolution. See also Fisher et al. (2009), in the Annual Review of Microbiology. (KW)
- February 8, 2010: A team of American and Ecuadorian scientists working for the nonprofit organization Reptile and Amphibian Ecology International, has discovered an estimated 30 new species of rain frogs (genus Pristimantis) at a site in coastal Ecuador. Nearly half of the new species come from a small cloudforest patch on Cerro Pata de Pájaro. Mountaintop cloudforests and surrounding rainforest usually contain high biodiversity and are under threat from both logging and climate change. For more Ecuadorian amphibians also see QCAZ's site AmphibiaWeb Ecuador (en Español). (KW)
- February 4, 2010: Why have some toads, such as the cane toad (Bufo marinus) been able to spread nearly worldwide? Van Bocxlaer et al. (2010), in a paper published in this week's issue of Science, uses phylogenetic analysis to define a suite of traits that are associated with range-expansion ability in the family Bufonidae. Those traits include the presence of parotoid glands, inguinal fat bodies (for energy storage), large adult size, lack of dependence on high humidity or constant accessibility of water bodies, large clutch size, ability to lay eggs in temporal water bodies, and exotrophous larvae (obtaining food from the environment rather than requiring maternal provisioning of extensive amounts of yolk). (KW)
- February 2, 2010: Can the Kihansi spray toad go back home? The New York Times describes conservation efforts for this Extinct in the Wild species, Nectophrynoides asperginis . This tiny toad species, which gives birth to miniscule toadlets, was discovered in 1998 living in the spray zone of a single Tanzanian waterfall. By 2000 a dam had been constructed that cut off 90% of the water, and most of the toad population died off soon thereafter. Chytrid fungus invaded and killed many of the remaining toads. In a bid to save this rare live-bearing species, scientists airlifted some survivors to zoos, where it was touch-and-go until it could be figured out how to keep them alive in captivity. This year some may be reintroduced to the wild. (KW)
- February 1, 2010: The Center for Biological Diversity last week petitioned the California Fish and Game Commission to list all populations of mountain yellow-legged frogs (Rana muscosa, in the southern Sierra and Transverse Ranges, and Rana sierrae, in the central and northern Sierra) as endangered. Once these frogs were the most abundant amphibians in the Sierras; surveys since 1995 show that they have been extirpated from nearly 95% of 225 historically known localities. Currently only the Southern California population of Rana muscosa is listed as endangered. (KW)
- January 25, 2010: 2010 is the International Year of Biodiversity! Amphibians are the most threatened vertebrate taxon, with at least 42% of species declining in numbers and nearly a third already threatened with extinction or lost entirely (Stuart et al. 2004). Please help conserve habitat, fund fieldwork and conservation, and become more aware of your local amphibians and the particular threats they face. (KW)
- January 18, 2010:
Was it fraud? Or the first demonstration of epigenetics? Vargas et al. (2009) takes a new look at the controversy surrounding Paul Kammerer's work on midwife toads, eighty-four years after Kammerer's suicide, and proposes a possible mechanism to account for Kammerer's results. See also Wagner's (2009) editorial in the same issue of the Journal of Experimental Zoology Part B: Molecular Development and Evolution, and Pennisi's (2009) commentary in Science.
But see also Whittaker (1975), who found that Kammerer's results on siphon regeneration in the ascidian Ciona intestinalis were not repeatable and were probably fraudulent. Kammerer had claimed these experiments were the best proof of his theory.
Whittaker also provided an insightful commentary in a later MBL publication: Whittaker, J. R. (1985). Paul Kammerer and the Suspect Siphons. MBL Science, published by Marine Biological Laboratory, Woods Hole, available online. (KW)
- January 13, 2010: Behavioral thermoregulation may be an important mechanism for amphibians to resist amphibian chytrid fungus infection. Richards-Zawacki (2010) showed that wild Panamanian golden frogs (Atelopus zeteki) had elevated body temperatures during a chytridiomycosis epidemic, regardless of air temperature, and that this affected chytrid fungal infection prevalence. (KW)
- January 4, 2010: A new paper by Weinstein (2009) discusses chytridiomycosis in a terrestrial direct-developing plethodontid salamander, Batrachoseps attenuatus. Among other findings, infected salamanders had 100% mortality when held under cool and moist conditions but shed the fungus under dry conditions similar to summer estivation. (KW)
- December 16, 2009: AmphibiaWeb wishes you a very happy holiday season! Check out our new website usage statistics (21,517 queries/day for 2009). Please help us celebrate (and continue as a website) by including us in your holiday gift-giving. (KW)
- December 7, 2009: A new paper on amphibian evolution (Sigurdsen and Bolt 2009) shows that salamanders and frogs share elbow morphology with the fossil caecilian Eocaecilia. In turn this synapomorphy is shared only with the Paleozoic temnospondyls, and not with lepospondyls, lending support to the monophyletic origin of lissamphibians from temnospondyls. (KW)
- November 29, 2009:
Sampling for amphibian chytrid fungus in wild Asian amphibians has not revealed much until this year, despite modeling predictions that Asia should be hospitable to chytrid. Now Goka et al. (2009) report that amphibian chytrid fungus appears to be both endemic and introduced in Japan, with high infection prevalence and unique, diverse chytrid haplotypes in native salamanders Andrias japonicus and Cynops ensicauda, as well as introduced species. (KW)
- November 22, 2009: A new population of Rana muscosa has been discovered in the San Jacinto Wilderness, and trout removal from a creek in Angeles National Forest is helping another small population of R. muscosa. See Lewis (2009). (KW)
- November 17, 2009: Please write the National Park Service by Saturday, November 21st, 2009, to support removal of non-native trout from lakes in Sequoia & King's Canyon National Park (U.S.A.). The scientific data are quite clear: when trout are removed, yellow-legged frog populations rebound. See Vredenburg (2004) and Knapp et al. (2007). (KW)
November 9, 2009:
A new paper in Science has reported how chytrid fungus kills frogs. Voyles et al. (2009) show that Batrachochytrium dendrobatidis infection compromises the electrolyte transport capability of frog skin, eventually depleting blood levels of potassium and sodium to the point where the Bd-infected frog dies from cardiac arrest. (KW)
Anchukaitis, K. J., and Evans, M. N. 2010. Tropical cloud forest climate variability and the demise of the Monteverde golden toad. Proceedings of the National Academy of Sciences, published online before print, http://www.pnas.org/content/early/2010/02/25/0908572107.abstract.
Bai, C., Garner, T. W. J., and Li, Y. 2010. First evidence of Batrachochytrium dendrobatidis in China: discovery of chytridiomycosis in introduced American bullfrogs and native amphibians in the Yunnan Province, China. EcoHealth 7(1): 127-134.
Barej, M. F., Schmitz, A., Menegon, M., Hillers, A., Hinkel, H., Böhme, W., and Rödel, M. O. 2011. Dusted off-the African Amietophrynus superciliaris-species complex of giant toads. Zootaxa 2772: 1-32,
Bell, R.C, Gata Garcia, A.V., Stuart, B.L., and Zamudio, K.R. 2011. High prevalence of the amphibian chytrid pathogen in Gabon. EcoHealth DOI: 10.1007/s10393-010-0364-4.
Blackburn, D. C., Evans, B. J., Pessier, A. P., and Vredenburg, V. T. 2010. An enigmatic mortality event in the only population of the Critically Endangered Cameroonian frog Xenopus longipes. African Journal of Herpetology 59 (1): 1-12, first published online on 29 July 2010.
Briggs, C. J., Knapp, R. A., and Vredenburg, V. T. 2010. Enzootic and epizootic dynamics of the chytrid fungal pathogen of amphibians. Proceedings of the National Academy of Sciences, published online before print, May 10, 2010, www.pnas.org/cgi/doi/10.1073/pnas.0912886107.
Catenazzi, A., Lehr, E., Rodriguez, L. O., and Vredenburg, V. T. 2011. Batrachochytrium dendrobatidis and the collapse of anuran species richness and abundance in the Upper Manu National Park, Southeastern Peru. Conservation Biology, in press, DOI: 10.1111/j.1523-1739.2010.01604.x.
Che, J., Zhou, W.-W., Hu, J.-S., Yan, F., Papenfuss, T. J., Wake, D. B., and Zhang, Y.-P. 2010. Spiny frogs (Paini) illuminate the history of the Himalayan region and Southeast Asia. Proceedings of the National Academy of Sciences of the United States of America, 107: 13765-13770. Available online.
Crawford, A. J., Lips, K. R., and Bermingham, E. 2010. Epidemic disease decimates amphibian abundance, species diversity, and evolutionary history in the highlands of central Panama. Proceedings of the National Academy of Sciences, published online before print, July 19, 2010, doi: 10.1073/pnas.0914115107.
Das, I., and Haas, A. 2010. New species of Microhyla from Sarawak: Old Worlds smallest frogs crawl out of miniature pitcher plants on Borneo (Amphibia: Anura: Microhylidae). Zootaxa, 271, 37-52.
Fisher, M. P., Garner, T. W. J., and Walker, S. F. 2009. Global emergence of Batrachochytrium dendrobatidis and amphibian chytridiomycosis in space, time, and host. Annual Review of Microbiology 63: 291310.
Glaw, F., and Vences, M. 1994. A Field Guide to the Amphibians and Reptiles of Madagascar, 2nd edition. Vences & Glaw Verlag, Bonn.
Goka, K., Yokoyama, J., Une, Y., Kuroki, T., Suzuki, K., Nakahara, M., Kobayashi, A., Inaba, S., Mizutani, T., and Hyatt, A. D. 2009. Amphibian chytridiomycosis in Japan: distribution, haplotypes, and possible route of entry into Japan. Molecular Ecology 18: 4757-4774.
Han, B.A., Searle, C.L., and Blaustein, A.R. (2011). Effects of an Infectious Fungus, Batrachochytrium dendrobatidis, on Amphibian Predator-Prey Interactions. PLoS ONE 6(2): e16675. doi:10.1371/journal.pone.0016675.
Hayes, T. B., Falso, P., Gallipeau, S., and Stice, M. 2010. The cause of global amphibian declines: a developmental endocrinologist's perspective. Journal of Experimental Biology 213: 921-933.
Hayes, T. B., Khoury, V., Narayan, A., Nazir, M., Park, A., Brown, T., Adame, L., Chan, E., Buchholz, D., Stueve, T., and Gallipeau, S. 2010. Atrazine induces complete feminization and chemical castration in male African clawed frogs (Xenopus laevis). Proceedings of the National Academy of Sciences, published online before print March 1, 2010, doi:10.1073/pnas.0909519107.
Hellsten, U., Harland, R. M., Gilchrist, M. J., and others. (2010). The genome of the Western Clawed Frog Xenopus tropicalis. Science 328: 633-636.
Herrel, A. and Measey, G. J. (2010). The kinematics of locomotion in caecilians: effects of substrate and body shape. Journal of Experimental Zoology A (Genetics and Physiology) 313A: 301-309.
Kilpatrick, A. M., Briggs, C. J., and Daszak, P. 2009. The ecology and impact of chytridiomycosis: an emerging disease of amphibians. Trends in Ecology and Evolution 25: 109-118.
Knapp, R., Boiano, D. M., and Vredenburg, V. T. 2007. Recovery of a declining amphibian (mountain yellow-legged frog, Rana muscosa) following removal of nonnative fish. Biological Conservation 135: 11-20. Available online.
Kohler, J., Vences, M., D'Cruze, N., and Glaw, F. 2010. Giant dwarfs: discovery of a radiation of large-bodied stump-toed frogs from karstic cave environments of northern Madagascar. Journal of Zoology 282: 21-38.
Lewis, T. 2009. New population of mountain yellow-legged frog (Rana muscosa) discovered. Herpetological Bulletin 108: 1-2. Available online.
Longo, A. V., and Burrowes, P. 2010. Persistence with chytridiomycosis does not assure survival of direct-developing frogs. Ecohealth, published online before print, June 29, 2010, doi: 10.1007/s10393-010-0327-9.
Martin, R. A., and Pfennig, D. W. (2010). Maternal investment influences expression of resource polymorphism in amphibians: implications for the evolution of novel resource-use phenotypes. PLoS ONE 5(2): e9117. doi:10.1371/journal.pone.0009117
Meegaskumbura, M., Meegaskumbura, S., Bowatte, G., Manamendra-Arachchi, K., Pethiyagoda, R., Hanken, J., and Schneider, C. J. 2010. Taruga (Anura: Rhacophoridae), a new genus of foam-nesting tree frogs endemic to Sri Lanka. Ceylon Journal of Science 39: 75-94.
Natale, G. S., Alcalde, L., Herrera, R., Cajade, R., Schaefer, E. F., Marangoni, F., and Trudeau, V. L. 2010. Underwater acoustic communication in the macrophagic carnivorous larvae of Ceratophrys ornata (Anura: Ceratophryidae). Acta Zoologica, published online before print, February 26, 2010, doi: 10.1111/j.1463-6395.2009.00445.x.
Onn, C. K., and Grismer, L. L. 2010. Re-assessment of the Reinwardts Gliding Frog, Rhacophorus reinwardtii (Schlegel 1840) (Anura: Rhacophoridae) in Southern Thailand and Peninsular Malaysia and its re-description as a new species. Zootaxa 2505: 4050.
Pennisi, E. 2009. The case of the midwife toad: fraud or epigenetics? Science 325 (5945): 1194-1195.
Petherick, A. A solar salamander. Nature News doi:10.1038/news.2010.384.
Phillimore, A. B., Hadfield, J. D., Jones, O. R., and Smithers, R. J. 2010. Differences in spawning date between populations of common frog reveal local adaptation. Proceedings of the National Academy of Sciences, published online before print, April 19, 2010, doi: 10.1073/pnas.0913792107.
Pounds, J. A., Bustamante, M. R., Coloma, L. A., Consuegra, J. A., Fogden, M. P. L., Foster, P. N., La Marca, E., Masters, K. L., Merino-Viteri, A., Puschendorf, R., Ron, S. R., Sánchez-Azofeifa, G. A., Still, C. J., and Young, B. E. 2006. Widespread amphibian extinctions from epidemic disease driven by global warming. Nature 439: 161-167.
Renner, R. 2008. Atrazine effects in Xenopus aren't reproducible. Environmental Science & Technology. 42: 3491-3493.
Richards-Zawacki, C. L. 2010. Thermoregulatory behaviour affects prevalence of chytrid fungal infection in a wild population of Panamanian golden frogs. Proceedings of the Royal Society B 277: 519-528.
Rodrigues, A. S. L., Gray, C. L., Crowter, B. J., Ewers, R. M., Stuart, S. N., Whitten, T., and Manica, A. 2010. A global assessment of amphibian taxonomic effort and expertise. Bioscience 60: 798-806.
Rodríguez, A., Poth, D., Schulz, S., and Vences, M. 2010. Discovery of skin alkaloids in a miniaturized eleutherodactylid frog from Cuba. Biology Letters, published online 3 November 2010 ahead of print, doi: 10.1098/rsbl.2010.0844
Rowley, J. J. L., Duong, L. T. T., Dao, T. T. A., Stuart, B. L., and Huy, H. D. 2010. A new tree frog of the genus Rhacophorus (Anura: Rhacophoridae) from southern Vietnam. Zootaxa 2727: 45-55.
Schloegel, L. M., Picco, A. M., Kilpatrick, A. M., Davies, A. J., Hyatt, A. D., and Daszak, P. 2009. Magnitude of the US trade in amphibians and presence of Batrachochytrium dendrobatidis and ranavirus infection in imported North American bullfrogs (Rana catesbeiana). Biological Conservation 142: 1420-1426.
Sigurdsen, T., and Bolt, J. R. 2009. The lissamphibian humerus and elbow joint, and the origins of modern amphibians. Journal of Morphology 270: 1443-1453. Abstract available online.
Sinervo, B., Méndez-de-la-Cruz, F., Miles, D. B., Heulin, B., Bastiaans, E., Villagrán-Santa Cruz, M., Lara-Resendiz, R., Martínez-Méndez, N., Calderón-Espinosa, M. L., Meza-Lázaro, R. N., Gadsden, H., Avila, L. J., Morando, M., De la Riva, I. J., Sepulveda, P. V., Rocha, C. F. D., Ibargüengoytía, N., Puntriano, C. A., Massot, M., Lepetz, V., Oksanen, T. A., Chapple, D. G., Bauer, A. M., Branch, W. R., Clobert, J., and Sites, J. W. Jr. 2010. Erosion of lizard diversity by climate change and altered thermal niches. Science 328: 894-899.
Stuart, S. N., Chanson, J. S., Cox, N. A., Young, B. E., Rodrigues, A. S. L., Fischman, D. L., and Waller, R. W. 2004. Status and trends of amphibian declines and extinctions worldwide. Science 306: 1783-1786.
Tingley, R., Romagosa, C. M., Kraus, F., Bickford, D., Philips, B. L., and Shine, R. 2010. The frog filter: amphibian introduction bias driven by taxonomy, body size and biogeography. Global Ecology and Biogeography 19: 496-503.
Todd, B. D., Scott, D. E., Pechmann, J. H. K., and Gibbons, J. W. 2010. Climate change correlates with rapid delays and advancements in reproductive timing in an amphibian community. Proceedings of the Royal Society B, published online before print December 15, 2010, doi: 10.1098/rspb.2010.1768.
Trudeau, V. L., Somoza, G. M., Natale, G. S., Pauli, B., Wignall, J., Jackman, P., Doe, K., and Schueler, F. W. 2010. Hormonal induction of spawning in 4 species of frogs by coinjection with a gonadotropin-releasing hormone agonist and a dopamine antagonist. Reproductive Biology and Endocrinology 8: doi:10.1186/1477-7827-8-36.
Van Bocxlaer, I., Loader, S. P., Roelants, K., Biju, S. D., Menegon, M., and Bossuyt, F. 2010. Gradual adaptation toward a range-Expansion phenotype initiated the global radiation of toads. Science 327(5966): 679-682.
Vargas, A. O. 2009. Did Paul Kammerer discover epigenetic inheritance? a modern look at the controversial midwife toad experiments. Journal of Experimental Zoology Part B (Molecular Development and Evolution) 312B: 667-678.
Vera Candioti, M. F., and Altig, R. 2010. A survey of shape variation in keratinized labial teeth of anuran larvae as related to phylogeny and ecology. Biological Journal of the Linnaean Society 101: 609-625.
Voituron, Y., de Fraipont, M., Issartel, J., Guillaume, O., and Clobert, J. 2010. Extreme lifespan of the human fish (Proteus anguinus): a challenge for ageing mechanisms. Biology Letters, published online before print July 21, 2010, doi: 10.1098/rsbl.2010.0539.
Voyles, J., Young, S., Berger, L., Campbell, C., Voyles, W. V., Dinudom, A., Cook, D., Webb, R., Alford, R. A., Skerratt, L. F., and Speare, R. 2009. Pathogenesis of chytridiomycosis, a cause of catastrophic amphibian declines. Science 326 (5952): 582-585. Available online.
Vredenburg, V. T. 2004. Reversing introduced species effects: Experimental removal of introduced fish leads to rapid recovery of declining frog. Proceedings of the National Academy of Sciences 101(20):7646-7650. Available online.
Vredenburg, V. T., Knapp, R. A., Tunstall, T. S., and Briggs, C. J. 2010. Dynamics of an emerging disease drive large-scale amphibian population extinctions. Proceedings of the National Academy of Sciences, published online before print May 10, 2010, www.pnas.org/cgi/doi/10.1073/pnas.0914111107.
Wagner, G. P. 2009. Paul Kammerers midwife toads: about the reliability of experiments and our ability to make sense of them. Journal of Experimental Zoology Part B (Molecular Development and Evolution) 312B: 665-666.
Walker, S. F., Bosch, J., Gomez, V., Garner, T. W. J., Cunningham, A. A., Schmeller, D. S., Ninyerola, M., Henk, D. A., Arthur, C. G. C.-P., and Fisher, M. C. 2010. Factors driving pathogenicity vs. prevalence of amphibian panzootic chytridiomycosis in Iberia. Ecology Letters doi: 10.1111/j.1461-0248.2009.01434.x
Weinstein, S. B. 2009. An aquatic disease on a terrestrial salamander: individual and population level effects of the amphibian chytrid fungus, Batrachochytrium dendrobatidis, on Batrachoseps attenuatus (Plethodontidae). Copeia 2009: 653-660.
Weisbecker, V. and Mitgutsch, C. 2010. A large-scale survey of heterochrony in anuran cranial ossification patterns. Journal of Zoological Systematics and Evolutionary Research 48: 332-347.
Wendell, D., Todd, J., and Montemagno, C. (2010). ''Artificial photosynthesis in Ranaspumin-2 based foam.'' Nano Letters, DOI: 10.1021/nl100550k.
Whittaker, J. R. 1975. Siphon regeneration in Ciona. Nature 255: 224-225.